{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:45:29Z","timestamp":1768506329223,"version":"3.49.0"},"posted":{"date-parts":[[2023,5,26]]},"group-title":"Microbiology","reference-count":61,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,5,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  During primary infection, varicella zoster virus (VZV) infects epithelial cells in the respiratory lymphoid organs and mucosa. Subsequent infection of lymphocytes, T cells in particular, causes primary viremia allowing systemic spread throughout the host, including the skin. This results in the expression of cytokines, including interferons (IFNs) which partly limit primary infection. VZV also spreads from skin keratinocytes to lymphocytes prior to secondary viremia. How VZV infects lymphocytes from epithelial cells while evading the cytokine response has not been fully established. Here, we show that VZV glycoprotein C (gC) binds IFN-\u03b3 and modifies its activity. Transcriptomic analysis revealed that gC in combination with IFN-\u03b3 increased the expression of a small subset of IFN-stimulated genes (ISGs), including intercellular adhesion molecule 1 (\n                  <jats:italic>ICAM1<\/jats:italic>\n                  ), as well as several chemokines and immunomodulatory genes. The higher ICAM1 protein level at the plasma membrane of epithelial cells resulted in lymphocyte function-associated antigen 1 (LFA-1)-dependent T cell adhesion. This gC activity required a stable interaction with IFN-\u03b3 and signalling through the IFN-\u03b3 receptor. Finally, the presence of gC during infection increased VZV spread from epithelial cells to peripheral blood mononuclear cells. This constitutes the discovery of a novel strategy to modulate the activity of IFN-\u03b3, inducing the expression of a subset of ISGs, leading to enhanced T cell adhesion and virus spread.\n                <\/jats:p>","DOI":"10.1101\/2023.05.26.542397","type":"posted-content","created":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T00:00:13Z","timestamp":1685145613000},"source":"Crossref","is-referenced-by-count":1,"title":["Viral modulation of type II interferon increases T cell adhesion and virus spread"],"prefix":"10.64898","author":[{"given":"Carina","family":"J\u00fcrgens","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Ssebyatika","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Beyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Pl\u00fcckebaum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai A.","family":"Kropp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00edctor","family":"Gonz\u00e1lez-Motos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Ritter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heike","family":"B\u00f6ning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eirini","family":"Nikolouli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul R.","family":"Kinchington","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nico","family":"Lachmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Pearce","family":"Depledge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Krey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6395-4010","authenticated-orcid":false,"given":"Abel","family":"Viejo-Borbolla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023053009001374000_2023.05.26.542397v1.1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.22.11425-11433.2002"},{"key":"2023053009001374000_2023.05.26.542397v1.2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.69.9.5236-5242.1995"},{"key":"2023053009001374000_2023.05.26.542397v1.3","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.13.6183-6192.2001"},{"key":"2023053009001374000_2023.05.26.542397v1.4","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.celrep.2014.06.024","article-title":"Single-cell mass cytometry analysis of human tonsil T cell remodeling by varicella zoster virus","volume":"8","year":"2014","journal-title":"Cell reports"},{"key":"2023053009001374000_2023.05.26.542397v1.5","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20040634"},{"key":"2023053009001374000_2023.05.26.542397v1.6","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.5.2651-2658.2005"},{"key":"2023053009001374000_2023.05.26.542397v1.7","doi-asserted-by":"publisher","DOI":"10.1007\/82_2010_29"},{"key":"2023053009001374000_2023.05.26.542397v1.8","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5349.381"},{"key":"2023053009001374000_2023.05.26.542397v1.9","doi-asserted-by":"crossref","unstructured":"Harjunpaa H , Llort Asens M , Guenther C , Fagerholm SC . 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