{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:44:50Z","timestamp":1791812690756,"version":"4.3.4"},"posted":{"date-parts":[[2023,5,29]]},"group-title":"Neuroscience","reference-count":78,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,2,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Heterozygous loss-of-function mutations in the progranulin gene (\n                  <jats:italic>GRN<\/jats:italic>\n                  ) are a major cause of frontotemporal dementia due to progranulin haploinsufficiency; complete deficiency of progranulin causes neuronal ceroid lipofuscinosis. Several progranulin-deficient mouse models have been generated, including both knockout mice and knockin mice harboring a common patient mutation (R493X). However, the\n                  <jats:italic>\n                    Grn\n                    <jats:sup>R493X<\/jats:sup>\n                  <\/jats:italic>\n                  mouse model has not been characterized completely. Additionally, while homozygous\n                  <jats:italic>\n                    Grn\n                    <jats:sup>R493X<\/jats:sup>\n                  <\/jats:italic>\n                  and\n                  <jats:italic>Grn<\/jats:italic>\n                  knockout mice have been extensively studied, data from heterozygous mice is still limited. Here, we performed more in-depth characterization of heterozygous and homozygous\n                  <jats:italic>\n                    Grn\n                    <jats:sup>R493X<\/jats:sup>\n                  <\/jats:italic>\n                  knockin mice, which includes biochemical assessments, behavioral studies, and analysis of fluid biomarkers. In the brains of homozygous\n                  <jats:italic>\n                    Grn\n                    <jats:sup>R493X<\/jats:sup>\n                  <\/jats:italic>\n                  mice, we found increased phosphorylated TDP-43 along with increased expression of lysosomal genes, markers of microgliosis and astrogliosis, pro-inflammatory cytokines, and complement factors. Heterozygous\n                  <jats:italic>\n                    Grn\n                    <jats:sup>R493X<\/jats:sup>\n                  <\/jats:italic>\n                  mice did not have increased TDP-43 phosphorylation but did exhibit limited increases in lysosomal and inflammatory gene expression. Behavioral studies found social and emotional deficits in\n                  <jats:italic>\n                    Grn\n                    <jats:sup>R493X<\/jats:sup>\n                  <\/jats:italic>\n                  mice that mirror those observed in\n                  <jats:italic>Grn<\/jats:italic>\n                  knockout mouse models, as well as impairment in memory and executive function. Overall, the\n                  <jats:italic>\n                    Grn\n                    <jats:sup>R493X<\/jats:sup>\n                  <\/jats:italic>\n                  knockin mouse model closely phenocopies\n                  <jats:italic>Grn<\/jats:italic>\n                  knockout models. Lastly, in contrast to homozygous knockin mice, heterozygous\n                  <jats:italic>\n                    Grn\n                    <jats:sup>R493X<\/jats:sup>\n                  <\/jats:italic>\n                  mice do not have elevated levels of fluid biomarkers previously identified in humans, including neurofilament light chain (NfL) and glial fibrillary acidic protein (GFAP) in both plasma and CSF. These results may help to inform pre-clinical studies that use this\n                  <jats:italic>Grn<\/jats:italic>\n                  knockin mouse model and other\n                  <jats:italic>Grn<\/jats:italic>\n                  knockout models.\n                <\/jats:p>","DOI":"10.1101\/2023.05.27.542495","type":"posted-content","created":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T20:25:27Z","timestamp":1685391927000},"source":"Crossref","is-referenced-by-count":4,"title":["Biochemical, biomarker, and behavioral characterization of the\n                  <i>\n                    Grn\n                    <sup>R493X<\/sup>\n                  <\/i>\n                  mouse model of frontotemporal dementia"],"prefix":"10.64898","author":[{"given":"Denise 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