{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:51:11Z","timestamp":1768506671122,"version":"3.49.0"},"posted":{"date-parts":[[2023,6,9]]},"group-title":"Neuroscience","reference-count":15,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,6,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The vertebrate hypothalamus regulates physiological and behavioral responses to environmental stimuli through the function of evolutionarily-conserved neuronal subpopulations. Our previous work found that mutation of zebrafish\n                  <jats:italic>lef1<\/jats:italic>\n                  , which encodes a transcriptional mediator of the Wnt signaling pathway, leads to the loss of hypothalamic neurons and behavioral phenotypes that are both associated with stress-related human mood disorders However, the specific Lef1 target genes that link neurogenesis to behavior remain unknown. One candidate is\n                  <jats:italic>otpb<\/jats:italic>\n                  , which encodes a transcription factor with known roles in hypothalamic development. Here we show that\n                  <jats:italic>otpb<\/jats:italic>\n                  expression in the posterior hypothalamus is Lef1-dependent, and that like\n                  <jats:italic>lef1<\/jats:italic>\n                  , its function is required for the generation of\n                  <jats:italic>crhbp<\/jats:italic>\n                  + neurons in this region. Transgenic reporter analysis of a\n                  <jats:italic>crhbp<\/jats:italic>\n                  conserved noncoding element suggests that\n                  <jats:italic>otpb<\/jats:italic>\n                  participates in a transcriptional regulatory network with other Lef1 targets. Finally, consistent with a role for\n                  <jats:italic>crhbp<\/jats:italic>\n                  in inhibiting the stress response, zebrafish\n                  <jats:italic>otpb<\/jats:italic>\n                  mutants exhibit decreased exploration in a novel tank diving assay. Together our findings suggest a potential evolutionarily-conserved mechanism for the regulation of innate stress response behaviors through Lef1-mediated hypothalamic neurogenesis.\n                <\/jats:p>","DOI":"10.1101\/2023.06.07.544119","type":"posted-content","created":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T15:10:16Z","timestamp":1686323416000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>otpb<\/i>\n                  functions in a Lef1-dependent transcriptional network required for expression of the stress response inhibitor\n                  <i>crhbp<\/i>\n                  in the zebrafish hypothalamus"],"prefix":"10.64898","author":[{"given":"Priscilla","family":"Figueroa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangning","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cade","family":"Kartchner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debora","family":"Brito de Andrade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harrison","family":"Watters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ethan","family":"Crispell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard I.","family":"Dorsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023061314000572000_2023.06.07.544119v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2009.12.004"},{"key":"2023061314000572000_2023.06.07.544119v1.2","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.euroneuro.2017.09.004","article-title":"Nitric oxide interacts with monoamine oxidase to modulate aggression and anxiety-like behaviour","volume":"30","year":"2020","journal-title":"Eur Neuropsychopharmacol"},{"key":"2023061314000572000_2023.06.07.544119v1.3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075002"},{"key":"2023061314000572000_2023.06.07.544119v1.4","doi-asserted-by":"publisher","DOI":"10.1007\/s10709-008-9311-5"},{"key":"2023061314000572000_2023.06.07.544119v1.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.20.11595"},{"key":"2023061314000572000_2023.06.07.544119v1.6","doi-asserted-by":"publisher","DOI":"10.1101\/gr.229102. 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