{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:39:12Z","timestamp":1772156352997,"version":"3.50.1"},"posted":{"date-parts":[[2023,6,22]]},"group-title":"Cell Biology","reference-count":49,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Mammalian sperm delve into the female reproductive tract to fertilize the female gamete. The available information about how sperm regulate their motility during the final journey to the fertilization site is extremely limited. In this work, we investigated the structural and functional changes in the sperm flagellum after AE and during the interaction with the eggs. The evidence demonstrates that the double helix actin network surrounding the mitochondrial sheath of the midpiece undergoes structural changes prior to the motility cessation. This structural modification is accompanied by a decrease in diameter of the midpiece and is driven by intracellular calcium changes that occur concomitant with a reorganization of the actin helicoidal cortex. Midpiece contraction occurs in a subset of cells that undergo AE, live-cell imaging during in vitro fertilization showed that the midpiece contraction is required for motility cessation after fusion is initiated. These findings provide the first evidence of the F-actin network\u2019s role in regulating sperm motility, adapting its function to meet specific cellular requirements during fertilization, and highlighting the broader significance of understanding sperm motility.<\/jats:p>\n                <jats:sec>\n                  <jats:title>Significant statement<\/jats:title>\n                  <jats:p>In this work, we demonstrate that the helical structure of polymerized actin in the flagellum undergoes a rearrangement at the time of sperm-egg fusion. This process is driven by intracellular calcium and promotes a decrease in the sperm midpiece diameter as well as the arrest in motility, which is observed after the fusion process is initiated.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2023.06.22.546073","type":"posted-content","created":{"date-parts":[[2023,6,24]],"date-time":"2023-06-24T03:42:08Z","timestamp":1687578128000},"source":"Crossref","is-referenced-by-count":2,"title":["Reorganization of the Flagellum Scaffolding Induces a Sperm Standstill During Fertilization"],"prefix":"10.64898","author":[{"given":"Martina","family":"Jablo\u00f1ski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermina M.","family":"Luque","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mat\u00edas D.","family":"G\u00f3mez-El\u00edas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Sanchez-Cardenas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinran","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose Luis","family":"de la Vega-Beltran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5979-9978","authenticated-orcid":false,"given":"Gabriel","family":"Corkidi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Linares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor X.","family":"Abonza Amaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aquetzalli","family":"Arenas-Hernandez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Del Pilar Ramos-Godinez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"L\u00f3pez-Saavedra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7607-1954","authenticated-orcid":false,"given":"Dario","family":"Krapf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2833-5553","authenticated-orcid":false,"given":"Diego","family":"Krapf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2502-0505","authenticated-orcid":false,"given":"Alberto","family":"Darszon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adan","family":"Guerrero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7163-6482","authenticated-orcid":false,"given":"Mariano G.","family":"Buffone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024070416100631000_2023.06.22.546073v4.1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod46.5.779"},{"key":"2024070416100631000_2023.06.22.546073v4.2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.116.140400"},{"key":"2024070416100631000_2023.06.22.546073v4.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2016.02.006"},{"key":"2024070416100631000_2023.06.22.546073v4.4","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.115.135368"},{"key":"2024070416100631000_2023.06.22.546073v4.5","doi-asserted-by":"publisher","DOI":"10.1038\/nature03362"},{"key":"2024070416100631000_2023.06.22.546073v4.6","doi-asserted-by":"crossref","unstructured":"T. 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