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Using the apicomplexan model\n                  <jats:italic>Toxoplasma gondii<\/jats:italic>\n                  , we show that host cell proteins including the transferrin receptor 1, transferrin, ferritin heavy and light chains, and clathrin light chain are robustly taken up by tachyzoites. Tachyzoite acquisition of host cell protein was not related to host cell type or parasite virulence phenotypes. Bradyzoites possessed little capacity to acquire host cell proteins consistent with the cyst wall representing a barrier to host cell protein cargo. Increased trafficking of host cell transferrin receptor 1 and transferrin to endolysosomes boosted tachyzoite acquisition of host proteins and growth rate. Theft of host transferrin 1 and transferrin did not significantly affect iron levels in the tachyzoite. This study provides insight into essential functions associated with parasite theft of host iron sequestration and storage proteins.\n                <\/jats:p>","DOI":"10.1101\/2023.06.23.546322","type":"posted-content","created":{"date-parts":[[2023,6,24]],"date-time":"2023-06-24T23:00:11Z","timestamp":1687647611000},"source":"Crossref","is-referenced-by-count":0,"title":["Theft of Host Transferrin Receptor-1 by\n                  <i>Toxoplasma gondii<\/i>\n                  is required for infection"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0779-2031","authenticated-orcid":false,"given":"Stephen L.","family":"Denton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexa","family":"Mejia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lindsay L.","family":"Nevarez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9314-4833","authenticated-orcid":false,"given":"Miguel P.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara A.","family":"Fox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8592-4733","authenticated-orcid":false,"given":"David J.","family":"Bzik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8805-4561","authenticated-orcid":false,"given":"Jason P.","family":"Gigley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023062810050988000_2023.06.23.546322v1.1","doi-asserted-by":"crossref","first-page":"17050","DOI":"10.1038\/nrdp.2017.50","article-title":"Malaria","volume":"3","year":"2017","journal-title":"Nature Reviews Disease Primers"},{"key":"2023062810050988000_2023.06.23.546322v1.2","doi-asserted-by":"crossref","first-page":"R193","DOI":"10.1016\/j.cub.2017.11.070","article-title":"Cryptosporidium","volume":"28","year":"2018","journal-title":"Current biology : CB"},{"key":"2023062810050988000_2023.06.23.546322v1.3","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.2.267"},{"key":"2023062810050988000_2023.06.23.546322v1.4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.2.509"},{"key":"2023062810050988000_2023.06.23.546322v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.01.056"},{"key":"2023062810050988000_2023.06.23.546322v1.6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010138"},{"key":"2023062810050988000_2023.06.23.546322v1.7","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36571-4"},{"key":"2023062810050988000_2023.06.23.546322v1.8","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1038\/s41467-023-37431-x","article-title":"Stable endocytic structures navigate the complex pellicle of apicomplexan parasites","volume":"14","year":"2023","journal-title":"Nat Commun"},{"key":"2023062810050988000_2023.06.23.546322v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2015.01.021"},{"key":"2023062810050988000_2023.06.23.546322v1.10","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1128\/MMBR.00012-07","article-title":"Siderophore-based iron acquisition and pathogen control","volume":"71","year":"2007","journal-title":"Microbiology and molecular biology reviews : MMBR"},{"key":"2023062810050988000_2023.06.23.546322v1.11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1988.tb00029.x"},{"key":"2023062810050988000_2023.06.23.546322v1.12","doi-asserted-by":"crossref","first-page":"3982","DOI":"10.1128\/iai.59.11.3982-3988.1991","article-title":"Identification and purification of transferrin- and lactoferrin-binding proteins of Bordetella pertussis and Bordetella bronchiseptica","volume":"59","year":"1991","journal-title":"Infection and immunity"},{"key":"2023062810050988000_2023.06.23.546322v1.13","doi-asserted-by":"crossref","first-page":"111361","DOI":"10.1016\/j.molbiopara.2021.111361","article-title":"Trypanosoma brucei transferrin receptor: Functional replacement of the GPI anchor with a transmembrane domain","volume":"242","year":"2021","journal-title":"Molecular and biochemical parasitology"},{"key":"2023062810050988000_2023.06.23.546322v1.14","first-page":"4446","volume":"5","year":"2014","journal-title":"Host iron status and iron supplementation mediate susceptibility to erythrocytic stage Plasmodium falciparum"},{"key":"2023062810050988000_2023.06.23.546322v1.15","doi-asserted-by":"crossref","unstructured":"Oliveira MC , Coutinho LB , Almeida MPO , Briceno MP , Araujo ECB , Silva NM . 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