{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T20:20:07Z","timestamp":1783542007404,"version":"3.55.0"},"posted":{"date-parts":[[2023,6,28]]},"group-title":"Neuroscience","reference-count":60,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:00:00Z","timestamp":1687910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["RF1AG039452"],"award-info":[{"award-number":["RF1AG039452"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["CRSK-3_195902"],"award-info":[{"award-number":["CRSK-3_195902"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["PZ00P3_216225"],"award-info":[{"award-number":["PZ00P3_216225"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,1,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Brain pericytes maintain the blood-brain barrier (BBB), secrete neurotrophic factors and clear toxic proteins. Their loss in neurological disorders leads to BBB breakdown, neuronal dysfunction, and cognitive decline. Therefore, cell therapy to replace lost pericytes holds potential to restore impaired cerebrovascular and brain functions. However, the molecular composition and function of human iPSC-derived brain pericytes (iPSC-PC) remains poorly characterized. Here, we show by a quantitative analysis of 8,344 proteins and 20,572 phosphopeptides that iPSC-PC share 96% of total proteins and 98% of protein phosphorylation sites with primary human brain pericytes. This includes cell adhesion and tight junction proteins, transcription factors, and different protein kinase families of the human kinome. In pericyte-deficient mice, iPSC-PC home to host brain capillaries to form hybrid human-mouse microvessels with ligand-receptor associations. They repair BBB leaks and protect against neuron loss, which we show requires PDGRFB and pleiotrophin. They also clear Alzheimer\u2019s amyloid-\u03b2 and tau neurotoxins in an\n                  <jats:italic>ex vivo<\/jats:italic>\n                  brain slice assay via lipoprotein receptor. Thus, iPSC-PC may have potential as a replacement therapy for pericyte-deficient neurological disorders.\n                <\/jats:p>","DOI":"10.1101\/2023.06.26.546577","type":"posted-content","created":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T18:55:16Z","timestamp":1687978516000},"source":"Crossref","is-referenced-by-count":15,"title":["Molecular signature and functional properties of human pluripotent stem cell-derived brain pericytes"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3376-3453","authenticated-orcid":false,"given":"Ruslan","family":"Rust","sequence":"first","affiliation":[{"name":"Department of Physiology and Neuroscience, University of Southern California, Los Angeles, CA 90089"},{"name":"Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, 1501 San Pablo St., Los Angeles, CA 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Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089"},{"name":"Department of Molecular Pathobiology, New York University, New York, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoming","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Neuroscience, University of Southern California, Los Angeles, CA 90089"},{"name":"Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, 1501 San Pablo St., Los Angeles, CA 90089"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Clementel","sequence":"additional","affiliation":[{"name":"Department of Physiology and Neuroscience, University of Southern California, Los Angeles, CA 90089"},{"name":"Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, 1501 San Pablo St., Los Angeles, CA 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