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The HCAb editing platform is highly flexible, supporting antigen-binding domains based on both antibody and non-antibody components, and also allowing alterations in the Fc domain. Using HIV Env protein as a model antigen, we show that B cells edited to express anti-Env HCAbs support the regulated expression of both BCRs and antibodies, and respond to Env antigen in a tonsil organoid model of immunization. In this way, human B cells can be reprogrammed to produce customized therapeutic molecules with the potential for\n                  <jats:italic>in vivo<\/jats:italic>\n                  amplification.\n                <\/jats:p>","DOI":"10.1101\/2023.06.28.546944","type":"posted-content","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T21:00:12Z","timestamp":1688158812000},"source":"Crossref","is-referenced-by-count":0,"title":["Reprogramming human B cells with custom heavy chain antibodies"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9183-1269","authenticated-orcid":false,"given":"Geoffrey L.","family":"Rogers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atishay","family":"Mathur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsu-Yu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalya","family":"Stanten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidy","family":"Morales","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan-Hua","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric J.","family":"Kezirian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0059-354X","authenticated-orcid":false,"given":"Paula M.","family":"Cannon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023070523250463000_2023.06.28.546944v1.1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1146\/annurev-chembioeng-101121-084508","article-title":"Outsmarting Pathogens with Antibody Engineering","volume":"14","year":"2023","journal-title":"Annu Rev Chem Biomol Eng"},{"key":"2023070523250463000_2023.06.28.546944v1.2","doi-asserted-by":"crossref","first-page":"2789","DOI":"10.1016\/j.cell.2022.05.029","article-title":"Designing antibodies as therapeutics","volume":"185","year":"2022","journal-title":"Cell"},{"key":"2023070523250463000_2023.06.28.546944v1.3","doi-asserted-by":"crossref","unstructured":"Page, A. , Fusil, F. & Cosset, F.L . 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