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We collected data in a simulated desert environment and a captive colony of cactus mice (\n                  <jats:italic>Peromyscus eremicus<\/jats:italic>\n                  ) and used lab-based experiments with real time physiological measurements to characterize the response to water-deprivation. Mice without access to water had significantly lower energy expenditures and in turn, reduced water loss compared to mice with access to water after the first 24 hours of the experiment. Additionally, we observed significant weight loss likely related to dehydration-associated anorexia a response to limit fluid loss by reducing waste and the solute load as well as allowing water reabsorption from the kidneys and gastrointestinal tract. Finally, we observed body temperature correlated with sex, with males without access to water maintaining body temperature when compared to hydrated males while body temperature decreased for females without access to water compared to hydrated, suggesting daily torpor in females.\n                <\/jats:p>","DOI":"10.1101\/2023.07.03.547568","type":"posted-content","created":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T03:05:11Z","timestamp":1688439911000},"source":"Crossref","is-referenced-by-count":2,"title":["When the tap runs dry: The physiological effects of acute experimental dehydration in\n                  <i>Peromyscus eremicus<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2129-5541","authenticated-orcid":false,"given":"Danielle M.","family":"Blumstein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2368-6960","authenticated-orcid":false,"given":"Matthew D.","family":"MacManes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023070801100873000_2023.07.03.547568v1.1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1613625114"},{"key":"2023070801100873000_2023.07.03.547568v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.1948.21"},{"key":"2023070801100873000_2023.07.03.547568v1.3","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.06813"},{"key":"2023070801100873000_2023.07.03.547568v1.4","doi-asserted-by":"crossref","unstructured":"Blumstein, D. 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