{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:39:17Z","timestamp":1772156357220,"version":"3.50.1"},"posted":{"date-parts":[[2023,7,9]]},"group-title":"Developmental Biology","reference-count":52,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,7,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Fraser Syndrome is a rare, multisystemic autosomal recessive disorder characterized by disrupted epithelial-mesenchymal associations upon loss of Fraser Complex genes. Disease manifestation and affected organs are highly variable. Digit malformations such as syndactyly are common but of unclear developmental origins. We explored if zebrafish\n                  <jats:italic>fraser extracellular matrix complex subunit 1 (fras1)<\/jats:italic>\n                  mutants model Fraser Syndrome-associated appendicular skeleton patterning defects. Approximately 10% of\n                  <jats:italic>fras1<\/jats:italic>\n                  mutants survive to adulthood, displaying striking and varied fin abnormalities, including endochondral bone fusions, ectopic cartilage, and disrupted caudal fin symmetry. The fins of surviving\n                  <jats:italic>fras1<\/jats:italic>\n                  mutants frequently have fewer and unbranched bony rays.\n                  <jats:italic>fras1<\/jats:italic>\n                  mutant fins regenerate to their original size but with exacerbated ray branching and fin symmetry defects. Single cell RNA-Seq analysis,\n                  <jats:italic>in situ<\/jats:italic>\n                  hybridizations, and antibody staining show specific Fraser complex expression in the basal epidermis during regenerative outgrowth. Fras1 and Fraser Complex component Frem2 accumulate along the basal side of distal-most basal epidermal cells. Greatly reduced and mislocalized Frem2 accompanies loss of Fras1 in\n                  <jats:italic>fras1<\/jats:italic>\n                  mutants. The Sonic hedgehog signaling between distal basal epidermis and adjacent mesenchymal pre-osteoblasts that promotes ray branching persists upon Fraser Complex loss. However,\n                  <jats:italic>fras1<\/jats:italic>\n                  mutant regenerating fins exhibit extensive sub-epidermal blistering associated with a disorganized basal epidermis and adjacent pre-osteoblasts. We propose Fraser Complex-supported tissue layer adhesion enables robust integrated tissue morphogenesis involving the basal epidermis and osteoblasts. Further, we establish zebrafish fin development and regeneration as an accessible model to explore mechanisms of Fraser Syndrome-associated digit defects and Fraser Complex function at epithelial-mesenchymal interfaces.\n                <\/jats:p>","DOI":"10.1101\/2023.07.08.548238","type":"posted-content","created":{"date-parts":[[2023,7,9]],"date-time":"2023-07-09T17:45:13Z","timestamp":1688924713000},"source":"Crossref","is-referenced-by-count":2,"title":["The Fraser complex interconnects tissue layers to support basal epidermis and osteoblast integrated morphogenesis underlying fin skeletal patterning"],"prefix":"10.64898","author":[{"given":"Amy E.","family":"Robbins","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel G.","family":"Horst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor M.","family":"Lewis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Stewart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6206-0365","authenticated-orcid":false,"given":"Kryn","family":"Stankunas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023071305150499000_2023.07.08.548238v1.1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.143792"},{"key":"2023071305150499000_2023.07.08.548238v1.2","unstructured":"Arratia, G. , Schultze, H.-P. , Wilson, M.V.H ., 2008. Actinopterygian postcranial skeleton with special reference to the diversity of fin ray elements, and the problem of identifying homologies, in: Mesozoic Fishes 4 \u2013 Homology and Phylogeny. pp. 49\u2013101."},{"key":"2023071305150499000_2023.07.08.548238v1.3","doi-asserted-by":"crossref","unstructured":"Bensimon-Brito, A. , Cardeira, J. , Dion\u00edsio, G. , Huysseune, A. , Cancela, M.L. , Witten, P.E ., 2016. Revisiting in vivo staining with alizarin red S - A valuable approach to analyse zebrafish skeletal mineralization during development and regeneration. 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