{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:54:04Z","timestamp":1768506844115,"version":"3.49.0"},"posted":{"date-parts":[[2023,7,11]]},"group-title":"Developmental Biology","reference-count":145,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,7,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Ocular lens development entails epithelial to fiber cell differentiation, defects in which cause congenital cataract. We report the first single-cell multiomic atlas of lens development, leveraging snRNA-seq, snATAC-seq, and CUT&amp;RUN-seq to discover novel mechanisms of cell fate determination and cataract-linked regulatory networks. A comprehensive profile of\n                  <jats:italic>cis<\/jats:italic>\n                  - and\n                  <jats:italic>trans<\/jats:italic>\n                  -regulatory interactions, including for the cataract-linked transcription factor MAF, is established across a temporal trajectory of fiber cell differentiation. Further, we divulge a conserved epigenetic paradigm of cellular differentiation, defined by progressive loss of H3K27 methylation writer Polycomb repressive complex 2 (PRC2). PRC2 localizes to heterochromatin domains across master-regulator transcription factor gene bodies, suggesting it safeguards epithelial cell fate. Moreover, we demonstrate that FGF hyper-stimulation\n                  <jats:italic>in vivo<\/jats:italic>\n                  leads to MAF network activation and the emergence of novel lens cell states. Collectively, these data depict a comprehensive portrait of lens fiber cell differentiation, while defining regulatory effectors of cell identity and cataract formation.\n                <\/jats:p>","DOI":"10.1101\/2023.07.10.548451","type":"posted-content","created":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T06:30:28Z","timestamp":1689057028000},"source":"Crossref","is-referenced-by-count":0,"title":["Integrated single-cell multiomics uncovers foundational regulatory mechanisms of lens development and pathology"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0224-2893","authenticated-orcid":false,"given":"Jared A","family":"Tangeman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia 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