{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:58:52Z","timestamp":1768507132781,"version":"3.49.0"},"posted":{"date-parts":[[2023,7,13]]},"group-title":"Microbiology","reference-count":38,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,7,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  There are currently no approved vaccines against the opportunistic pathogen\n                  <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                  . Among vaccine targets, the lipopolysaccharide (LPS) O antigen of\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  is the most immunodominant protective candidate. There are twenty different O antigens composed of different repeat sugars structures conferring serogroup specificity, and ten are found most frequently in infection. Thus, one approach to combat infection by\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  could be to generate immunity with a vaccine cocktail that includes all these serogroups. Serogroup O9 is one of the ten serogroups commonly found in infection, but it has never been developed into a vaccine, likely due, in part, to the acid labile nature of the O9 polysaccharide. Our laboratory has previously shown that intranasal administration of an attenuated\n                  <jats:italic>Salmonella<\/jats:italic>\n                  strain expressing the\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  serogroup O11 LPS O antigen was effective in clearing and preventing mortality in mice following intranasal challenge with serogroup O11\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  . Consequently, we set out to develop a\n                  <jats:italic>P<\/jats:italic>\n                  .\n                  <jats:italic>aeruginosa<\/jats:italic>\n                  serogroup O9 vaccine using a similar approach. Here we show that\n                  <jats:italic>Salmonella<\/jats:italic>\n                  expressing serogroup O9 triggered an antibody-mediated immune response following intranasal administration to mice and that it conferred protection from\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  serogroup O9 in a murine model of acute pneumonia.\n                <\/jats:p>","DOI":"10.1101\/2023.07.13.548830","type":"posted-content","created":{"date-parts":[[2023,7,14]],"date-time":"2023-07-14T00:55:12Z","timestamp":1689296112000},"source":"Crossref","is-referenced-by-count":1,"title":["Efficacy of a\n                  <i>Pseudomonas aeruginosa<\/i>\n                  Serogroup O9 Vaccine"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9616-5947","authenticated-orcid":false,"given":"Dina A.","family":"Moustafa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1745-647X","authenticated-orcid":false,"given":"Antonio","family":"DiGiandomenico","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishnu","family":"Raghuram","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Schulman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer M.","family":"Scarff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Michael R.","family":"Davis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4868-1971","authenticated-orcid":false,"given":"John J.","family":"Varga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles R.","family":"Dean","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5285-5188","authenticated-orcid":false,"given":"Joanna B.","family":"Goldberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023071805251002000_2023.07.13.548830v1.1","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12033"},{"key":"2023071805251002000_2023.07.13.548830v1.2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000860"},{"key":"2023071805251002000_2023.07.13.548830v1.3","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.2014.890053"},{"key":"2023071805251002000_2023.07.13.548830v1.4","doi-asserted-by":"crossref","unstructured":"Sainz-Mejias M , Jurado-Martin I , McClean S . 2020. 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