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Here, we aim to address these broader questions through the study of intestinal looping, a process by which the initially straight intestinal tube buckles into loops, permitting ordered packing within the body cavity. Looping results from elongation of the tube against the constraint of an attached tissue, the dorsal mesentery, which is elastically stretched by the elongating tube to nearly triple its length. This elastic energy storage allows the mesentery to provide stable compressive forces that ultimately buckle the tube into loops. Beginning with a transcriptomic analysis of the mesentery, we identified widespread upregulation of extracellular matrix related genes during looping, including genes related to elastic fiber deposition. Combining molecular and mechanical analyses, we conclude that elastin confers tensile stiffness to the mesentery, enabling its mechanical role in organizing the developing small intestine. These results shed light on the role of elastin as a driver of morphogenesis that extends beyond its more established role in resisting cyclic deformation in adult tissues.<\/jats:p>","DOI":"10.1101\/2023.07.18.549562","type":"posted-content","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T16:43:51Z","timestamp":1689785031000},"source":"Crossref","is-referenced-by-count":0,"title":["Elastic fibers define embryonic tissue stiffness to enable buckling morphogenesis of the small intestine"],"prefix":"10.64898","author":[{"given":"Elise A.","family":"Loffet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F.","family":"Durel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Kam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyunjee","family":"Lim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nandan L.","family":"Nerurkar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023072120550613000_2023.07.18.549562v1.1","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.devcel.2020.11.025","article-title":"Mechanics of Development","volume":"56","year":"2021","journal-title":"Dev. 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