{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T01:25:04Z","timestamp":1774315504681,"version":"3.50.1"},"posted":{"date-parts":[[2023,7,19]]},"group-title":"Microbiology","reference-count":62,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,7,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The possible active entry of aminoglycosides in bacterial cells has been debated since the development of this antibiotic family. Here we report the identification of their active transport mechanism in\n                  <jats:italic>Vibrio<\/jats:italic>\n                  species. We combined genome-wide transcriptional analysis and fitness screens to identify alterations driven by treatment of\n                  <jats:italic>V. cholerae<\/jats:italic>\n                  with sub-minimum inhibitory concentrations (sub-MIC) of the aminoglycoside tobramycin. RNA-seq data showed downregulation of the small non-coding RNA\n                  <jats:italic>ncRNA586<\/jats:italic>\n                  during such treatment, while Tn-seq revealed that inactivation of this sRNA was associated with improved fitness in the presence of tobramycin. This sRNA is located near sugar transport genes and previous work on a homologous region in\n                  <jats:italic>Vibrio tasmaniensis<\/jats:italic>\n                  suggested that this sRNA stabilizes gene transcripts for carbohydrate transport and utilization, as well as phage receptors. The role for\n                  <jats:italic>ncRNA586<\/jats:italic>\n                  , hereafter named\n                  <jats:italic>ctrR<\/jats:italic>\n                  , in the transport of both carbohydrates and aminoglycosides, was further investigated. Flow cytometry on cells treated with a fluorescent aminoglycoside confirmed the role of\n                  <jats:italic>ctrR<\/jats:italic>\n                  and of carbohydrate transporters in differential aminoglycoside entry. Despite sequence diversity,\n                  <jats:italic>ctrR<\/jats:italic>\n                  showed functional conservation across the Vibrionales. This system in directly modulated by carbon sources, suggesting regulation by carbon catabolite repression, a widely conserved mechanism in Gram-negative bacteria, priming future research on aminoglycoside uptake by sugar transporters in other bacterial species.\n                <\/jats:p>","DOI":"10.1101\/2023.07.19.549712","type":"posted-content","created":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T00:45:12Z","timestamp":1689813912000},"source":"Crossref","is-referenced-by-count":4,"title":["Identification of the active mechanism of aminoglycoside entry in\n                  <i>V. cholerae<\/i>\n                  through characterization of sRNA\n                  <i>ctrR,<\/i>\n                  regulating carbohydrate utilization and transport"],"prefix":"10.64898","author":[{"given":"Sebastian A.","family":"Pierl\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7843-4747","authenticated-orcid":false,"given":"Manon","family":"Lang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roc\u00edo","family":"L\u00f3pez-Igual","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evelyne","family":"Krin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2170-3536","authenticated-orcid":false,"given":"Dominique","family":"Fourmy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean P.","family":"Kennedy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7097-9072","authenticated-orcid":false,"given":"Marie-Eve","family":"Val","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3477-2685","authenticated-orcid":false,"given":"Zeynep","family":"Baharoglu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Didier","family":"Mazel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023072403000663000_2023.07.19.549712v1.1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2011.140889"},{"key":"2023072403000663000_2023.07.19.549712v1.2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003421"},{"key":"2023072403000663000_2023.07.19.549712v1.3","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01549-10"},{"key":"2023072403000663000_2023.07.19.549712v1.4","doi-asserted-by":"crossref","unstructured":"Serio, A. 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