{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:59:55Z","timestamp":1768507195030,"version":"3.49.0"},"posted":{"date-parts":[[2023,7,20]]},"group-title":"Microbiology","reference-count":52,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,7,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Antimicrobial resistance (AMR) has been established to be a significant driver for the persistence and spread of bacterial infections. It is, therefore, essential to conduct epidemiological surveillance of AMR in healthy individuals to understand the actual dynamics of AMR in Nigeria. Multi-drug resistant\n                  <jats:italic>Klebsiella quasivariicola<\/jats:italic>\n                  (n=1)\n                  <jats:italic>, Enterobacter hormaechei<\/jats:italic>\n                  (n=1), and\n                  <jats:italic>Escherichia coli<\/jats:italic>\n                  (n=3) from stool samples of healthy children were subjected to whole genome sequencing using Illumina Nextseq1000\/2000 and Oxford nanopore. Bioinformatics analysis reveals antimicrobial resistance, virulence genes, and plasmids. This pathogenic enteric bacteria harbored more than three plasmid replicons of either Col and\/or Inc type associated with outbreaks and AMR resistant gene\n                  <jats:italic>pmrB<\/jats:italic>\n                  responsible for colistin resistance. Plasmid reconstruction revealed an integrated\n                  <jats:italic>tetA<\/jats:italic>\n                  gene responsible for tetracycline resistance, and\n                  <jats:italic>caa<\/jats:italic>\n                  gene responsible for toxin production in two of the\n                  <jats:italic>E.coli<\/jats:italic>\n                  isolates, and a\n                  <jats:italic>cusC<\/jats:italic>\n                  gene known to induce neonatal meningitis in the\n                  <jats:italic>K. quasivariicola<\/jats:italic>\n                  ST3879. The global spread of MDR pathogenic enteric bacteria is a worrying phenomenon, and close surveillance of healthy individuals, especially children, is strongly recommended to prevent the continuous spread and achieve the elimination and eradication of these infections. Molecular epidemiological surveillance using whole genome sequencing (WGS) will improve the detection of MDR pathogens in Nigeria.\n                <\/jats:p>","DOI":"10.1101\/2023.07.19.549742","type":"posted-content","created":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T00:20:20Z","timestamp":1689898820000},"source":"Crossref","is-referenced-by-count":1,"title":["Genomic Characterisation of Multidrug-Resistant Pathogenic Enteric Bacteria from healthy children in Osun State, Nigeria"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7916-2130","authenticated-orcid":false,"given":"Jessica N.","family":"Uwanibe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Idowu B.","family":"Olawoye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian T.","family":"Happi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Onikepe A.","family":"Folarin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023072411251647000_2023.07.19.549742v1.1","doi-asserted-by":"crossref","unstructured":"Rock C , Donnenberg MS . 2014. 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