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We combined reversible DNA handles with a norbornene-modified hyaluronic acid hydrogel to orthogonally add and remove multiple biomolecule-DNA conjugates at user-defined timepoints. We demonstrated that the persistent presentation of the cell adhesion peptide RGD was required to maintain cell spreading on hyaluronic acid hydrogels. Further, we discovered the delayed presentation of osteogenic growth peptide (OGP) increased alkaline phosphatase activity compared to other temporal variations. This finding is critically important when considering the design of OGP delivery approaches for bone repair. More broadly, this platform provides a unique approach to tease apart the temporal role of multiple biomolecules during development, regeneration, and disease progression.<\/jats:p>","DOI":"10.1101\/2023.07.19.549767","type":"posted-content","created":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T07:11:44Z","timestamp":1689837104000},"source":"Crossref","is-referenced-by-count":0,"title":["Using dynamic biomaterials to study the temporal role of osteogenic growth peptide during osteogenesis"],"prefix":"10.64898","author":[{"given":"Fallon M.","family":"Fumasi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tara","family":"MacCulloch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"Bernal-Chanchavac","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Stephanopoulos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8508-3041","authenticated-orcid":false,"given":"Julianne L.","family":"Holloway","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"issue":"8","key":"2023072207450458000_2023.07.19.549767v1.1","doi-asserted-by":"crossref","first-page":"2506","DOI":"10.1016\/j.bbagen.2014.01.010","article-title":"Extracellular Matrix: A Dynamic Microenvironment for Stem Cell Niche","volume":"1840","year":"2014","journal-title":"Biochim. 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