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We find that hydrophobic CDR3 residues promote regulatory T cell transcriptional states in both the CD8 and CD4 lineages. Most strikingly, we find a set of TCR sequence features, concentrated in CDR2\u03b1, that promotes positive selection in the thymus as well as transition from na\u00efve to memory in the periphery. Even among T cells that recognize the same antigen, these TCR sequence features help to explain which T cells form immunological memory, which is essential for effective pathogen response.<\/jats:p>","DOI":"10.1101\/2023.07.20.549939","type":"posted-content","created":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T00:30:12Z","timestamp":1690158612000},"source":"Crossref","is-referenced-by-count":4,"title":["The T cell receptor sequence influences the likelihood of T cell memory formation"],"prefix":"10.64898","author":[{"given":"Kaitlyn A.","family":"Lagattuta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aparna","family":"Nathan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurie","family":"Rumker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2281-3518","authenticated-orcid":false,"given":"Michael E.","family":"Birnbaum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1901-8265","authenticated-orcid":false,"given":"Soumya","family":"Raychaudhuri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023072511150680000_2023.07.20.549939v1.1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.13176"},{"key":"2023072511150680000_2023.07.20.549939v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3667"},{"key":"2023072511150680000_2023.07.20.549939v1.3","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.7.1149"},{"key":"2023072511150680000_2023.07.20.549939v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/83118"},{"key":"2023072511150680000_2023.07.20.549939v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2010.04.010"},{"key":"2023072511150680000_2023.07.20.549939v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2938"},{"key":"2023072511150680000_2023.07.20.549939v1.7","doi-asserted-by":"publisher","DOI":"10.1038\/nri3307"},{"key":"2023072511150680000_2023.07.20.549939v1.8","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.it.2022.01.002","article-title":"TCR-sequencing in cancer and autoimmunity: barcodes and beyond","volume":"43","year":"2022","journal-title":"Trends Immunol"},{"key":"2023072511150680000_2023.07.20.549939v1.9","doi-asserted-by":"publisher","DOI":"10.1038\/nature01433"},{"key":"2023072511150680000_2023.07.20.549939v1.10","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-022-01129-x"},{"key":"2023072511150680000_2023.07.20.549939v1.11","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.5.1287"},{"key":"2023072511150680000_2023.07.20.549939v1.12","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3491"},{"key":"2023072511150680000_2023.07.20.549939v1.13","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.6A0215-071RR"},{"key":"2023072511150680000_2023.07.20.549939v1.14","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01516"},{"key":"2023072511150680000_2023.07.20.549939v1.15","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.jaci.2019.03.022","article-title":"Cysteine and hydrophobic residues in CDR3 serve as distinct T-cell self-reactivity indices","volume":"144","year":"2019","journal-title":"J. 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