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This work shows that HTLV-1 employs an unconventional mechanism of Gag-Gag interactions to form the immature viral lattice. Analysis of high-resolution structural information from immature CA tubular arrays reveals that the primary stabilizing component in HTLV-1 is CA-NTD. Mutagenesis and biophysical analysis support this observation. This distinguishes HTLV-1 from other retroviruses, in which the stabilization is provided primarily by the CA-CTD. These results are the first to provide structural details of the quaternary arrangement of Gag for an immature deltaretrovirus, and this helps explain why HTLV-1 particles are morphologically distinct.\n                <\/jats:p>","DOI":"10.1101\/2023.07.24.548988","type":"posted-content","created":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T20:35:11Z","timestamp":1690230911000},"source":"Crossref","is-referenced-by-count":0,"title":["Unconventional stabilization of the human T-cell leukemia virus type 1 immature Gag lattice"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1756-6564","authenticated-orcid":false,"given":"Martin","family":"Obr","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4081-1571","authenticated-orcid":false,"given":"Mathias","family":"Percipalle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4866-8498","authenticated-orcid":false,"given":"Darya","family":"Chernikova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0539-0477","authenticated-orcid":false,"given":"Huixin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6966-0400","authenticated-orcid":false,"given":"Andreas","family":"Thader","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6727-7296","authenticated-orcid":false,"given":"Gergely","family":"Pinke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6180-3611","authenticated-orcid":false,"given":"Dario","family":"Porley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2391-1061","authenticated-orcid":false,"given":"Louis M","family":"Mansky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3693-2531","authenticated-orcid":false,"given":"Robert A","family":"Dick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4790-8078","authenticated-orcid":false,"given":"Florian KM","family":"Schur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023072611550666000_2023.07.24.548988v1.1","doi-asserted-by":"crossref","unstructured":"A. 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