{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:57:54Z","timestamp":1768507074811,"version":"3.49.0"},"posted":{"date-parts":[[2023,7,29]]},"group-title":"Biophysics","reference-count":15,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,7,29]]},"abstract":"<jats:label>1<\/jats:label>\n                <jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  A spatiotemporal model for antibiotic accumulation in bacterial biofilm microcolonies which leverages heterogenous porosity and attachment site profiles replicated the periphery sequestration phenomena reported in prior experimental studies on\n                  <jats:italic>Pseudomonas aeruginosa PAO1<\/jats:italic>\n                  biofilm cell clusters. These\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  cell clusters are\n                  <jats:italic>in vitro<\/jats:italic>\n                  models of the chronic\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  infections found in adult cystic fibrosis patients, which display resistance to antibiotic treatments, leading to exacerbated morbidity and mortality. This resistance has been partially attributed to periphery sequestration, where antibiotics are unable to penetrate biofilm cell clusters. The underlying physical phenomena driving this periphery sequestration have not been definitively established. This paper introduces mathematical models to account for two proposed physical phenomena driving periphery sequestration: biofilm matrix attachment and volume-exclusion due to variable biofilm porosity. An antibiotic accumulation model which incorporated these phenomena was able to better fit observed periphery sequestration data compared to previous models.\n                <\/jats:p>","DOI":"10.1101\/2023.07.28.551018","type":"posted-content","created":{"date-parts":[[2023,7,30]],"date-time":"2023-07-30T02:15:10Z","timestamp":1690683310000},"source":"Crossref","is-referenced-by-count":1,"title":["Heterogenous Biofilm Mass-Transport Model Replicates Periphery Sequestration of Antibiotics in\n                  <i>P. aeruginosa<\/i>\n                  PAO1 Microcolonies"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6050-9544","authenticated-orcid":false,"given":"Joshua","family":"Prince","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3840-8039","authenticated-orcid":false,"suffix":"III","given":"A-Andrew D.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023080114101485000_2023.07.28.551018v1.1","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1055\/s-0042-1758732","article-title":"Microbial Epidemiology of the Cystic Fibrosis Airways: Past, Present, and Future","volume":"44","year":"2023","journal-title":"Semin Respir Crit Care Med"},{"key":"2023080114101485000_2023.07.28.551018v1.2","first-page":"S5","article-title":"Increasing life expectancy in cystic fibrosis: Advances and challenges","volume":"57","year":"2022","journal-title":"Pediatric Pulmonology"},{"key":"2023080114101485000_2023.07.28.551018v1.3","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/s41579-022-00682-4","article-title":"Tolerance and resistance of microbial biofilms","volume":"20","year":"2022","journal-title":"Nat. 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