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More recently, machine learning has been incorporated into embryo selection \u201cpackages\u201d. Here, we report a machine-learning assisted embryo health assessment tool utilizing a quantitative phase imaging technique called artificial confocal microscopy (ACM). We present a label-free nucleus detection method with novel quantitative embryo health biomarkers. Two viability assessment models are presented for grading embryos into two classes: healthy\/intermediate (H\/I) or sick (S) class. The models achieve a weighted F1 score of 1.0 and 0.99 respectively on the in-distribution test set of 72 fixed embryos and a weighted F1 score of 0.9 and 0.95 respectively on the out-of-distribution test dataset of 19 time-instances from 8 live embryos.<\/jats:p>","DOI":"10.1101\/2023.07.30.550591","type":"posted-content","created":{"date-parts":[[2023,7,30]],"date-time":"2023-07-30T12:30:11Z","timestamp":1690720211000},"source":"Crossref","is-referenced-by-count":0,"title":["Machine learning assisted health viability assay for mouse embryos with artificial confocal microscopy (ACM)"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3000-1947","authenticated-orcid":false,"given":"Neha","family":"Goswami","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Winston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wonho","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nastasia Z. E.","family":"Lai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel B.","family":"Arcanjo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nahil","family":"Sobh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romana A.","family":"Nowak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Anastasio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Popescu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023080116200424000_2023.07.30.550591v1.1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22525"},{"key":"2023080116200424000_2023.07.30.550591v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4004(03)00136-X"},{"key":"2023080116200424000_2023.07.30.550591v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2012.10.021"},{"key":"2023080116200424000_2023.07.30.550591v1.4","doi-asserted-by":"crossref","first-page":"170","DOI":"10.2174\/1874120701004010170","article-title":"A review on automatic analysis of human embryo microscope images","volume":"4","year":"2010","journal-title":"The open biomedical engineering journal"},{"key":"2023080116200424000_2023.07.30.550591v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2020.09.157"},{"key":"2023080116200424000_2023.07.30.550591v1.6","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1093\/biolre\/ioz035","article-title":"Time-lapse imaging: the state of the art","volume":"101","year":"2019","journal-title":"Biology of Reproduction"},{"key":"2023080116200424000_2023.07.30.550591v1.7","doi-asserted-by":"crossref","unstructured":"Armstrong, S. et al. 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