{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:11:38Z","timestamp":1768507898805,"version":"3.49.0"},"posted":{"date-parts":[[2023,8,2]]},"group-title":"Microbiology","reference-count":71,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,8,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  <jats:italic>Chlamydia<\/jats:italic>\n                  , an obligate intracellular bacterial pathogen, has a unique developmental cycle involving the differentiation of invading elementary bodies (EBs) to noninfectious reticulate bodies (RBs), replication of RBs, and redifferentiation of RBs into progeny EBs. Progression of this cycle is regulated by three sigma factors, which direct the RNA polymerase to their respective target gene promoters. We hypothesized that the\n                  <jats:italic>Chlamydia-<\/jats:italic>\n                  specific transcriptional regulator GrgA, previously shown to activate \u03c366 and \u03c328, plays an essential role in chlamydial development and growth. To test this hypothesis, we applied a novel genetic tool known as dependence on plasmid-mediated expression (DOPE) to create\n                  <jats:italic>Chlamydia trachomatis<\/jats:italic>\n                  with conditional GrgA-deficiency. We show that GrgA-deficient\n                  <jats:italic>C. trachomatis<\/jats:italic>\n                  RBs have a growth rate that is approximately half of the normal rate and fail to transition into progeny EBs. In addition, GrgA-deficient\n                  <jats:italic>C. trachomatis<\/jats:italic>\n                  fail to maintain its virulence plasmid. Results of RNA-seq analysis indicate that GrgA promotes RB growth by optimizing tRNA synthesis and expression of nutrient-acquisition genes, while it enables RB-to-EB conversion by facilitating the expression of a histone and outer membrane proteins required for EB morphogenesis. GrgA also regulates numerous other late genes required for host cell exit and subsequent EB invasion into host cells. Importantly, GrgA stimulates the expression of \u03c354, the third and last sigma factor, and its activator AtoC, and thereby indirectly upregulating the expression of \u03c354-dependent genes. In conclusion, our work demonstrates that GrgA is a master transcriptional regulator in\n                  <jats:italic>Chlamydia<\/jats:italic>\n                  and plays multiple essential roles in chlamydial pathogenicity.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>IMPORTANCE<\/jats:title>\n                  <jats:p>\n                    Hallmarks of the developmental cycle of the obligate intracellular pathogenic bacterium\n                    <jats:italic>Chlamydia<\/jats:italic>\n                    are the primary differentiation of the infectious elementary body (EB) into the proliferative reticulate body (RB) and the secondary differentiation of RBs back into EBs. The mechanisms regulating these transitions remain unclear. In this report, we developed an effective novel strategy termed DOPE that allows for the knockdown of essential genes in\n                    <jats:italic>Chlamydia<\/jats:italic>\n                    . We demonstrate that GrgA, a\n                    <jats:italic>Chlamydia<\/jats:italic>\n                    -specific transcription factor, is essential for the secondary differentiation and optimal growth of RBs. We also show that GrgA, a chromosome-encoded regulatory protein, controls the maintenance of the chlamydial virulence plasmid. Transcriptomic analysis further indicates that GrgA functions as a critical regulator of all three sigma factors that recognize different promoter sets at developmental stages. The DOPE strategy outlined here should provide a valuable tool for future studies examining chlamydial growth, development, and pathogenicity.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2023.08.02.551707","type":"posted-content","created":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:30:10Z","timestamp":1691022610000},"source":"Crossref","is-referenced-by-count":0,"title":["Requirement of GrgA for\n                  <i>Chlamydia<\/i>\n                  infectious progeny production, optimal growth, and efficient plasmid maintenance"],"prefix":"10.64898","author":[{"given":"Bin","family":"Lu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wurihan","family":"Wurihan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sydney","family":"Yeung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph D.","family":"Fondell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao","family":"Lai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danny","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2087-2709","authenticated-orcid":false,"given":"Wei Vivian","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4903-926X","authenticated-orcid":false,"given":"Huizhou","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023080404100652000_2023.08.02.551707v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2005.03.002"},{"key":"2023080404100652000_2023.08.02.551707v1.2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.9.3937"},{"key":"2023080404100652000_2023.08.02.551707v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(90)90500-Q"},{"key":"2023080404100652000_2023.08.02.551707v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02432-0"},{"key":"2023080404100652000_2023.08.02.551707v1.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1331135100"},{"key":"2023080404100652000_2023.08.02.551707v1.6","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.10.3179-3189.2003"},{"key":"2023080404100652000_2023.08.02.551707v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(00)00185-8"},{"key":"2023080404100652000_2023.08.02.551707v1.8","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5389.754"},{"key":"2023080404100652000_2023.08.02.551707v1.9","doi-asserted-by":"publisher","DOI":"10.1101\/gr.7020108"},{"key":"2023080404100652000_2023.08.02.551707v1.10","doi-asserted-by":"crossref","unstructured":"Soules KR , LaBrie SD , May BH , Hefty PS . 2020. 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