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Human stem cells were genetically modified to mimic this mutation, generating cortical organoids that exhibited altered cell cycle dynamics. RNA sequencing of cortical organoids at day 32 unveiled changes in cell adhesion and WNT-signaling pathways. ChIP-seq of PRDM16 identified binding sites in postmortem human fetal cortex, indicating the conservation of PRDM16 binding to developmental genes in mice and humans, potentially at enhancer sites. A shared motif between PRDM16 and LHX2 was identified and further examined through comparison with LHX2 ChIP-seq data from mice. These results suggested a collaborative partnership between PRDM16 and LHX2 in regulating a common set of genes and pathways in cortical radial glia cells, possibly via their synergistic involvement in cortical development.<\/jats:p>","DOI":"10.1101\/2023.08.12.553065","type":"posted-content","created":{"date-parts":[[2023,8,13]],"date-time":"2023-08-13T04:05:10Z","timestamp":1691899510000},"source":"Crossref","is-referenced-by-count":3,"title":["PRDM16 co-operates with LHX2 to shape the human 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