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There is still significant uncertainty around the role of different tissue microenvironments in the immune response to biomaterials and how that may alter downstream scaffold remodeling and integration. In this study, we present a study evaluating the immune response to decellularized extracellular matrix materials within the intraperitoneal cavity, the subcutaneous space, and in a traumatic skeletal muscle injury microenvironment. All different locations induced robust cellular recruitment, specifically of macrophages and eosinophils. The latter was most prominent in the subcutaneous space. Intraperitoneal implants uniquely recruited B cells that may alter downstream reactivity as adaptive immunity has been strongly implicated in the outcome of scaffold remodeling. These data suggest that the location of tissue implants should be taken together with the composition of the material itself when designing devices for downline therapeutics.<\/jats:p>","DOI":"10.1101\/2023.08.15.553390","type":"posted-content","created":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T14:50:12Z","timestamp":1692283812000},"source":"Crossref","is-referenced-by-count":3,"title":["Conserved and tissue-specific immune responses to biologic scaffold implantation"],"prefix":"10.64898","author":[{"given":"Sabrina","family":"DeStefano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditya","family":"Josyula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mondreakest","family":"Faust","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daphna","family":"Fertil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi","family":"Lokwani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tran B.","family":"Ngo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2587-8330","authenticated-orcid":false,"given":"Kaitlyn","family":"Sadtler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"issue":"3","key":"2023082107500649000_2023.08.15.553390v1.1","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1016\/j.actbio.2011.11.031","article-title":"Macrophage phenotype as a predictor of constructive remodeling following the implantation of biologically derived surgical mesh materials","volume":"8","year":"2012","journal-title":"Acta biomaterialia"},{"key":"2023082107500649000_2023.08.15.553390v1.2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1300290110"},{"key":"2023082107500649000_2023.08.15.553390v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.02.053"},{"key":"2023082107500649000_2023.08.15.553390v1.4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1304046110"},{"key":"2023082107500649000_2023.08.15.553390v1.5","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad9272"},{"key":"2023082107500649000_2023.08.15.553390v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00319-2"},{"key":"2023082107500649000_2023.08.15.553390v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.11.002"},{"key":"2023082107500649000_2023.08.15.553390v1.8","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.matsci.31.1.81"},{"key":"2023082107500649000_2023.08.15.553390v1.9","doi-asserted-by":"crossref","unstructured":"J.M. 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