{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T12:08:21Z","timestamp":1768565301915,"version":"3.49.0"},"posted":{"date-parts":[[2023,9,1]]},"group-title":"Infectious Diseases (except HIV\/AIDS)","reference-count":40,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>\n                    New Delhi metallo-\u03b2-lactamase (NDM) represents an emergent mechanism of carbapenem resistance associated with high mortality and limited antimicrobial treatment options. Because the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>NDM<\/jats:sub>\n                    resistance gene is often carried on plasmids, traditional infection prevention and control (IP&amp;C) surveillance methods like speciation, antimicrobial resistance testing, and reactive whole genome sequencing (WGS) may not detect plasmid transfer in multispecies outbreaks.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Initial outbreak detection of NDM-producing Enterobacterales identified at an acute care hospital occurred via traditional IP&amp;C methods and was supplemented by real-time WGS surveillance, which was performed weekly using the Illumina platform. To resolve NDM-encoding plasmids, we performed long-read Oxford Nanopore sequencing and constructed hybrid assemblies using Illumina and Nanopore sequencing data. Reports of relatedness between NDM-producing organisms and reactive WGS for suspected outbreaks were shared with the IP&amp;C team for assessment and intervention.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Findings<\/jats:title>\n                  <jats:p>We observed a multispecies outbreak of NDM-5-producing Enterobacterales isolated from 15 patients between February 2021 and February 2023. The 19 clinical and surveillance isolates sequenced included seven bacterial species and each encoded the same NDM-5 plasmid, which showed high homology to NDM plasmids previously observed in Asia. WGS surveillance and epidemiologic investigation characterized ten horizontal plasmid transfer events and six bacterial transmission events between patients housed in varying hospital units. Transmission prevention focused on enhanced observation and adherence to basic infection prevention measures.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Interpretation<\/jats:title>\n                  <jats:p>Our investigation revealed a complex, multispecies outbreak of NDM that involved multiple plasmid transfer and bacterial transmission events, increasing the complexity of outbreak identification and transmission prevention. Our investigation highlights the utility of combining traditional IP&amp;C and prospective genomic methods in identifying and containing plasmid-associated outbreaks.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Funding<\/jats:title>\n                  <jats:p>This work was funded in part by the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH) (R01AI127472) (R21AI1783691).<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:label>5.<\/jats:label>\n                  <jats:title>Summary<\/jats:title>\n                  <jats:p>We investigated a multispecies outbreak of Enterobacterales harboring the same New-Delhi metallo-\u03b2-lactamase-encoding plasmid using both traditional infection prevention and genomic approaches. Our investigation revealed a complex outbreak involving 7 bacterial species, including both bacterial transmission and plasmid transfer events.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2023.08.31.23294545","type":"posted-content","created":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T16:25:34Z","timestamp":1693585534000},"source":"Crossref","is-referenced-by-count":1,"title":["Genomic Epidemiologic Investigation of a Multispecies Hospital Outbreak of NDM-5-Producing Enterobacterales Infections"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3258-9003","authenticated-orcid":false,"given":"Nathan J.","family":"Raabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abby L.","family":"Valek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marissa P.","family":"Griffith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma","family":"Mills","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kady","family":"Waggle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vatsala Rangachar","family":"Srinivasa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley M.","family":"Ayres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Bradford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Creager","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lora L.","family":"Pless","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander J.","family":"Sundermann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daria Van","family":"Tyne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham M.","family":"Snyder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3787-2705","authenticated-orcid":false,"given":"Lee H.","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023090608300439000_2023.08.31.23294545v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2018.06.007"},{"key":"2023090608300439000_2023.08.31.23294545v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1234"},{"key":"2023090608300439000_2023.08.31.23294545v1.3","doi-asserted-by":"crossref","unstructured":"Snyder BM , Montague BT , Anandan S , Madabhushi AG , Pragasam AK , Verghese VP , et al. 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