{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:06:10Z","timestamp":1768507570440,"version":"3.49.0"},"posted":{"date-parts":[[2023,9,1]]},"group-title":"Molecular Biology","reference-count":78,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  <jats:italic>Toxoplasma gondii<\/jats:italic>\n                  is a protozoan parasite that infects a broad spectrum of hosts and can colonize many organs and cell types. The ability to reside within a wide range of different niches requires substantial adaptability to diverse microenvironments. Very little is known about how this parasite senses various milieus and adapts its metabolism to survive, replicate during the acute stage, and then differentiate to the chronic stage. Most eukaryotes, from yeast to mammals, rely on a nutrient sensing machinery involving the TORC complex as master regulator of cell growth and cell cycle progression. The lysosome functions as a signaling hub where TORC complex assembles and is activated by transceptors, which both sense and transport amino acids, including the arginine transceptor SLC38A9. While most of the TORC components are lost in\n                  <jats:italic>T. gondii<\/jats:italic>\n                  , indicating the evolution of a distinct nutrient sensing mechanism, the parasite\u2019s lysosomal plant-like vacuolar compartment (PLVAC) may still serve as a sensory platform for controlling parasite growth and differentiation. Using SLC38A9 to query the\n                  <jats:italic>T. gondii<\/jats:italic>\n                  proteome, we identified four putative amino acid transporters, termed TgAAT1-4, that structurally resemble the SLC38A9 arginine transceptor. Assessing their expression and sub-cellular localization, we found that one of them, TgAAT1, localized to the PLVAC and is necessary for normal parasite extracellular survival and bradyzoite differentiation. Moreover, we show that TgAAT1 is involved in the PLVAC efflux of arginine, an amino acid playing a key role in\n                  <jats:italic>T. gondii<\/jats:italic>\n                  differentiation, further supporting the hypothesis that TgAAT1 might play a role in nutrient sensing.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>IMPORTANCE<\/jats:title>\n                  <jats:p>\n                    <jats:italic>T. gondii<\/jats:italic>\n                    is a highly successful parasite infecting a broad range of warm-blood organisms including about one third of all humans. Although\n                    <jats:italic>Toxoplasma<\/jats:italic>\n                    infections rarely result in symptomatic disease in individuals with a healthy immune system, the incredibly high number of persons infected along with the risk of severe infection in immunocompromised patients and the potential link of chronic infection to mental disorders make this infection a significant public health concern. As a result, there is a pressing need for new treatment approaches that are both effective and well-tolerated. The limitations in understanding how\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    manages its metabolism to adapt to changing environments and triggers its transformation into bradyzoites have hindered the discovery of vulnerabilities in its metabolic pathways or nutrient acquisition mechanisms to identify new therapeutic targets. In this work, we have shown that the lysosome-like organelle PLVAC, acting through the putative arginine transporter TgAAT1, plays a pivotal role in regulating the parasite\u2019s extracellular survival and differentiation into bradyzoites.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2023.08.31.555807","type":"posted-content","created":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T02:55:10Z","timestamp":1693623310000},"source":"Crossref","is-referenced-by-count":2,"title":["A\n                  <i>Toxoplasma gondii<\/i>\n                  putative arginine transporter localizes to the plant-like vacuolar compartment and controls parasite extracellular survival and stage differentiation"],"prefix":"10.64898","author":[{"given":"Federica","family":"Piro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Masci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geetha","family":"Kannan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riccardo","family":"Focaia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracey L.","family":"Schultz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6859-8895","authenticated-orcid":false,"given":"Vern 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