{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:33:43Z","timestamp":1778114023355,"version":"3.51.4"},"posted":{"date-parts":[[2023,9,9]]},"group-title":"Developmental Biology","reference-count":66,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T00:00:00Z","timestamp":1694217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,8,7]]},"abstract":"<jats:title>\n                  <jats:bold>Summary<\/jats:bold>\n                <\/jats:title>\n                <jats:p>\n                  Stem cells often rely on signals from a niche, which in many tissues adopts a precise morphology. What remains elusive is how niches are formed, and how morphology impacts function. To address this, we leverage the\n                  <jats:italic>Drosophila<\/jats:italic>\n                  gonadal niche, which affords genetic tractability and live-imaging. We have previously shown mechanisms dictating niche cell migration to their appropriate position within the gonad, and the resultant consequences on niche function. Here, we show that once positioned, niche cells robustly polarize filamentous actin (F-actin) and Non-muscle Myosin II (MyoII) towards neighboring germ cells. Actomyosin tension along the niche periphery generates a highly reproducible smoothened contour. Without contractility, niches are misshapen and exhibit defects in their ability to regulate germline stem cell behavior. We additionally show that germ cells aid in polarizing MyoII within niche cells, and that extrinsic input is required for niche morphogenesis and function. Our work reveals a feedback mechanism where stem cells shape the niche that guides their behavior.\n                <\/jats:p>","DOI":"10.1101\/2023.09.08.556877","type":"posted-content","created":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T15:15:12Z","timestamp":1694272512000},"source":"Crossref","is-referenced-by-count":7,"title":["An actomyosin network organizes niche morphology and responds to feedback from recruited stem cells"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2375-6498","authenticated-orcid":false,"given":"Bailey N.","family":"Warder","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4847-2835","authenticated-orcid":false,"given":"Kara A.","family":"Nelson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin","family":"Sui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5482-5882","authenticated-orcid":false,"given":"Lauren","family":"Anllo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4131-5511","authenticated-orcid":false,"given":"Stephen","family":"DiNardo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024080910501951000_2023.09.08.556877v3.1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3651"},{"key":"2024080910501951000_2023.09.08.556877v3.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2009.09.005"},{"key":"2024080910501951000_2023.09.08.556877v3.3","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-7037-4_11"},{"key":"2024080910501951000_2023.09.08.556877v3.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.03.038"},{"key":"2024080910501951000_2023.09.08.556877v3.5","doi-asserted-by":"publisher","DOI":"10.1016\/bs.asn.2019.06.002"},{"key":"2024080910501951000_2023.09.08.556877v3.6","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3721"},{"key":"2024080910501951000_2023.09.08.556877v3.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.03.024"},{"key":"2024080910501951000_2023.09.08.556877v3.8","doi-asserted-by":"crossref","unstructured":"Pentinmikko, N. , Lozano, R. , Scharaw, S. , Andersson, S. , Englund, J.I. , Castillo-Azofeifa, D. , Gallagher, A. , Broberg, M. , Song, K.-Y. , Sola Carvajal, A. , et al. 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