{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:19:10Z","timestamp":1768508350817,"version":"3.49.0"},"posted":{"date-parts":[[2023,10,15]]},"group-title":"Biochemistry","reference-count":49,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  <jats:italic>Candida albicans<\/jats:italic>\n                  , an opportunistic fungal human pathogen, is a major threat to the healthcare system due to both infections in immunocompromised individuals and the emergence of antifungal resistance. Fungal infection caused by\n                  <jats:italic>C. albicans<\/jats:italic>\n                  , candidiasis, is a life-threatening condition in immunocompromised patients and the current treatments are mostly restricted to polyenes, azoles, and echinocandins. Use of these antifungals is limited by toxicity, drug-drug interactions, and the emergence of resistance, underscoring the importance of identifying novel therapeutic targets and the need for new treatment approaches.\n                  <jats:italic>C. albicans<\/jats:italic>\n                  can undergo a morphological transition from yeast to hyphae and this transition is central to\n                  <jats:italic>C. albicans<\/jats:italic>\n                  virulence. Here, we determine the impact of sinefungin, a natural nucleoside analog of S-adenosyl methionine, on the virulence of\n                  <jats:italic>C. albicans<\/jats:italic>\n                  strain SC5314 by evaluating treatment effects on the morphological transition, human epithelial cell adhesion, and biofilm formation. Our data indicate that sinefungin impairs pathogenic traits of\n                  <jats:italic>C. albicans<\/jats:italic>\n                  including hyphal lengthening, biofilm formation and the adhesion to the human epithelial cell lines, without adversely affecting human cells, therefore highlighting sinefungin as a potential avenue for therapeutic intervention. We determine that the formation of N6-methyladenosine (m\n                  <jats:sup>6<\/jats:sup>\n                  A) is particularly disturbed by sinefungin. More broadly, this study underscores the importance of considering the post-transcriptional control mechanisms of pathogenicity when designing therapeutic solutions to fungal infection.\n                <\/jats:p>","DOI":"10.1101\/2023.10.12.562127","type":"posted-content","created":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T22:20:12Z","timestamp":1697408412000},"source":"Crossref","is-referenced-by-count":0,"title":["Sinefungin, a natural nucleoside analog of S-adenosyl methionine, impairs the pathogenicity of\n                  <i>Candida albicans<\/i>"],"prefix":"10.64898","author":[{"given":"Anushka","family":"Nayak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Chavarria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyla N.","family":"Sanders","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Homa","family":"Ghalei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sohail","family":"Khoshnevis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023101813351481000_2023.10.12.562127v1.1","first-page":"47","article-title":"Growing role of Candida albicans as an important cause of nosocomial infection","volume":"3","year":"2022","journal-title":"Journal of Advances in Microbiology 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