{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:30:47Z","timestamp":1768509047235,"version":"3.49.0"},"posted":{"date-parts":[[2023,10,23]]},"group-title":"Microbiology","reference-count":67,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,10,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Biofilms are communities of interacting microbes embedded in a matrix of polymer, protein, and other materials. Biofilms develop distinct mechanical characteristics that depend on their predominant matrix components. These matrix components may be produced by microbes themselves or, for infections\n                  <jats:italic>in vivo<\/jats:italic>\n                  , incorporated from the host environment.\n                  <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                  is a human pathogen that forms robust biofilms that extensively tolerate antibiotics and effectively evade clearance by the immune system. Two of the important bacterial-produced polymers in the matrices of\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  biofilms are alginate and extracellular DNA (eDNA), both of which are anionic and therefore have the potential to interact electrostatically with cations. Many physiological sites of infection contain significant concentrations of the calcium ion (Ca\n                  <jats:sup>2+<\/jats:sup>\n                  ). In this study we investigate the structural and mechanical impacts of Ca\n                  <jats:sup>2+<\/jats:sup>\n                  supplementation in alginate-dominated biofilms grown\n                  <jats:italic>in vitro<\/jats:italic>\n                  and we evaluate the impact of targeted enzyme treatments on clearance by immune cells. We use multiple particle tracking microrheology to evaluate the changes in biofilm viscoelasticity caused by treatment with alginate lyase and\/or DNAse I. For biofilms grown without Ca\n                  <jats:sup>2+<\/jats:sup>\n                  , we correlate a decrease in relative elasticity with increased phagocytic success. However, we find that growth with Ca\n                  <jats:sup>2+<\/jats:sup>\n                  supplementation disrupts this correlation except in the case where both enzymes are applied. This suggests that the calcium cation may be impacting the microstructure of the biofilm in non-trivial ways. Indeed, confocal laser scanning fluorescence microscopy and scanning electron microscopy reveal unique Ca\n                  <jats:sup>2+<\/jats:sup>\n                  -dependent eDNA and alginate microstructures. Our results suggest that the presence of Ca\n                  <jats:sup>2+<\/jats:sup>\n                  drives the formation of structurally and compositionally discrete microdomains within the biofilm through electrostatic interactions with the anionic matrix components eDNA and alginate. Further, we observe that these structures serve a protective function as the dissolution of both components is required to render biofilm bacteria vulnerable to phagocytosis by neutrophils.\n                <\/jats:p>\n                <jats:sec id=\"s55\">\n                  <jats:title>Abstract Figure<\/jats:title>\n                  <jats:fig id=\"ufig1\" position=\"float\" orientation=\"portrait\" fig-type=\"figure\">\n                    <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"563605v1_ufig1\" position=\"float\" orientation=\"portrait\"\/>\n                  <\/jats:fig>\n                <\/jats:sec>","DOI":"10.1101\/2023.10.23.563605","type":"posted-content","created":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T23:55:20Z","timestamp":1698105320000},"source":"Crossref","is-referenced-by-count":1,"title":["Physiological concentrations of calcium interact with alginate and extracellular DNA in the matrices of\n                  <i>Pseudomonas aeruginosa<\/i>\n                  biofilms to impede phagocytosis by neutrophils"],"prefix":"10.64898","author":[{"given":"Marilyn J.","family":"Wells","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hailey","family":"Currie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5989-9378","authenticated-orcid":false,"given":"Vernita D.","family":"Gordon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023102713501537000_2023.10.23.563605v1.1","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1093\/jpids\/piac052","article-title":"Opportunistic Pathogens in Cystic Fibrosis: Epidemiology and Pathogenesis of Lung Infection","volume":"11","year":"2022","journal-title":"J Pediatric Infect Dis Soc"},{"key":"2023102713501537000_2023.10.23.563605v1.2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1318"},{"key":"2023102713501537000_2023.10.23.563605v1.3","doi-asserted-by":"crossref","unstructured":"Martin, I. , V. 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