{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:39:36Z","timestamp":1772156376988,"version":"3.50.1"},"posted":{"date-parts":[[2023,10,30]]},"group-title":"Microbiology","reference-count":118,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,5,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Apicomplexan parasites balance proliferation, persistence, and spread in their metazoan hosts. AGC kinases, such as PKG, PKA, and the PDK1 ortholog SPARK, integrate environmental signals to toggle parasites between replicative and motile life stages. Recent studies have cataloged pathways downstream of apicomplexan PKG and PKA; however, less is known about the global integration of AGC kinase signaling cascades. Here, conditional genetics coupled to unbiased proteomics demonstrates that SPARK complexes with an elongin-like protein to regulate the stability of PKA and PKG in the model apicomplexan\n                  <jats:italic>Toxoplasma gondii<\/jats:italic>\n                  . Defects attributed to SPARK depletion develop after PKG and PKA are down-regulated. Parasites lacking SPARK differentiate into the chronic form of infection, which may arise from reduced activity of a coccidian-specific PKA ortholog. This work delineates the signaling topology of AGC kinases that together control transitions within the asexual cycle of this important family of parasites.\n                <\/jats:p>","DOI":"10.1101\/2023.10.30.564746","type":"posted-content","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T23:50:30Z","timestamp":1698709830000},"source":"Crossref","is-referenced-by-count":0,"title":["SPARK regulates AGC kinases central to the\n                  <i>Toxoplasma gondii<\/i>\n                  asexual cycle"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3368-0893","authenticated-orcid":false,"given":"Alice L.","family":"Herneisen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2919-2523","authenticated-orcid":false,"given":"Michelle L.","family":"Peters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3399-4043","authenticated-orcid":false,"given":"Tyler A.","family":"Smithh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7625-4772","authenticated-orcid":false,"given":"Emily","family":"Shortt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5237-1095","authenticated-orcid":false,"given":"Sebastian","family":"Lourido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024050608300933000_2023.10.30.564746v2.1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8285"},{"key":"2024050608300933000_2023.10.30.564746v2.2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00329-08"},{"key":"2024050608300933000_2023.10.30.564746v2.3","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1038\/s41586-023-06821-y","article-title":"In vitro production of cat-restricted Toxoplasma pre-sexual stages","volume":"625","year":"2024","journal-title":"Nature"},{"key":"2024050608300933000_2023.10.30.564746v2.4","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14634"},{"key":"2024050608300933000_2023.10.30.564746v2.5","doi-asserted-by":"crossref","first-page":"e0304222","DOI":"10.1128\/mbio.03042-22","article-title":"IMC29 Plays an Important Role in Toxoplasma Endodyogeny and Reveals New Components of the Daughter-Enriched IMC Proteome","volume":"14","year":"2023","journal-title":"MBio"},{"key":"2024050608300933000_2023.10.30.564746v2.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00572-x"},{"key":"2024050608300933000_2023.10.30.564746v2.7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1905558116"},{"key":"2024050608300933000_2023.10.30.564746v2.8","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abe5396"},{"key":"2024050608300933000_2023.10.30.564746v2.9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06249.x"},{"key":"2024050608300933000_2023.10.30.564746v2.10","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.TIR119.001906"},{"key":"2024050608300933000_2023.10.30.564746v2.11","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/s41564-018-0339-8","article-title":"Phosphatidic acid governs natural egress in Toxoplasma gondii via a guanylate cyclase receptor platform","volume":"4","year":"2019","journal-title":"Nat Microbiol"},{"key":"2024050608300933000_2023.10.30.564746v2.12","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-091014-104100"},{"key":"2024050608300933000_2023.10.30.564746v2.13","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4201"},{"key":"2024050608300933000_2023.10.30.564746v2.14","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20130103"},{"key":"2024050608300933000_2023.10.30.564746v2.15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001806"},{"key":"2024050608300933000_2023.10.30.564746v2.16","doi-asserted-by":"publisher","DOI":"10.3391\/mbi.2017.8.1.01"},{"key":"2024050608300933000_2023.10.30.564746v2.17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.700518"},{"key":"2024050608300933000_2023.10.30.564746v2.18","doi-asserted-by":"crossref","unstructured":"Brown KM , Tonkin CJ , Billker O , Sibley LD . 2020. 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