{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:15:56Z","timestamp":1768508156544,"version":"3.49.0"},"posted":{"date-parts":[[2023,11,3]]},"group-title":"Cell Biology","reference-count":51,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,11,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Cilia-derived extracellular vesicles (EVs) contain signaling proteins and act in intercellular communication. Polycystin-2 (PKD-2), a transient receptor potential channel, is a conserved ciliary EVs cargo.\n                  <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                  serves as a model for studying ciliary EV biogenesis and function.\n                  <jats:italic>C. elegans<\/jats:italic>\n                  males release EVs in a mechanically-induced manner and deposit PKD-2-labeled EVs onto the hermaphrodite vulva during mating, suggesting an active release process. Here, we study the dynamics of ciliary EV release using time-lapse imaging and find that cilia can sustain the release of PKD-2-labeled EVs for a two-hour duration. Intriguingly, this extended release doesn\u2019t require neuronal synaptic transmission. Instead, ciliary intrinsic mechanisms regulate PKD-2 ciliary membrane replenishment and dynamic EV release. The ciliary kinesin-3 motor KLP-6 is necessary for both initial and extended ciliary EV release, while the transition zone protein NPHP-4 is required only for sustained EV release. The dihydroceramide desaturase DEGS1\/2 ortholog TTM-5 is highly expressed in the EV-releasing sensory neurons, localizes to cilia, and is required for sustained but not initial ciliary EV release, implicating ceramide in ciliary ectocytosis. The study offers a comprehensive portrait of real-time ciliary EV release, and mechanisms supporting cilia as proficient EV release platforms.\n                <\/jats:p>","DOI":"10.1101\/2023.11.01.565151","type":"posted-content","created":{"date-parts":[[2023,11,4]],"date-time":"2023-11-04T01:00:11Z","timestamp":1699059611000},"source":"Crossref","is-referenced-by-count":1,"title":["Ciliary intrinsic mechanisms regulate dynamic ciliary extracellular vesicle release from sensory neurons"],"prefix":"10.64898","author":[{"given":"Juan","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josh","family":"Saul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inna A.","family":"Nikonorova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Nava","family":"Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaiden M.","family":"Power","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken C.","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H.","family":"Hall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maureen M.","family":"Barr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023110803051181000_2023.11.01.565151v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-022-00763-8"},{"key":"2023110803051181000_2023.11.01.565151v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-023-00576-0"},{"key":"2023110803051181000_2023.11.01.565151v1.3","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.246553"},{"key":"2023110803051181000_2023.11.01.565151v1.4","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20170085"},{"key":"2023110803051181000_2023.11.01.565151v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.04.019"},{"key":"2023110803051181000_2023.11.01.565151v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2014.01.002"},{"key":"2023110803051181000_2023.11.01.565151v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2023.06.002"},{"key":"2023110803051181000_2023.11.01.565151v1.8","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008060564"},{"key":"2023110803051181000_2023.11.01.565151v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.06.040"},{"key":"2023110803051181000_2023.11.01.565151v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.05.079"},{"key":"2023110803051181000_2023.11.01.565151v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.02.039"},{"key":"2023110803051181000_2023.11.01.565151v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.07.017"},{"key":"2023110803051181000_2023.11.01.565151v1.13","doi-asserted-by":"publisher","DOI":"10.1038\/14755"},{"key":"2023110803051181000_2023.11.01.565151v1.14","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1466-07.2007"},{"key":"2023110803051181000_2023.11.01.565151v1.15","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14177"},{"key":"2023110803051181000_2023.11.01.565151v1.16","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200707090"},{"key":"2023110803051181000_2023.11.01.565151v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2023.06.013"},{"key":"2023110803051181000_2023.11.01.565151v1.18","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232315317"},{"key":"2023110803051181000_2023.11.01.565151v1.19","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.13816"},{"key":"2023110803051181000_2023.11.01.565151v1.20","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11908-0"},{"key":"2023110803051181000_2023.11.01.565151v1.21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001314"},{"key":"2023110803051181000_2023.11.01.565151v1.22","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.117.300106"},{"key":"2023110803051181000_2023.11.01.565151v1.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.07.042"},{"key":"2023110803051181000_2023.11.01.565151v1.24","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.110.122879"},{"key":"2023110803051181000_2023.11.01.565151v1.25","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.10.057"},{"key":"2023110803051181000_2023.11.01.565151v1.26","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E15-01-0009"},{"key":"2023110803051181000_2023.11.01.565151v1.27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1010560"},{"key":"2023110803051181000_2023.11.01.565151v1.28","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2022.03.005"},{"key":"2023110803051181000_2023.11.01.565151v1.29","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-25542-1"},{"key":"2023110803051181000_2023.11.01.565151v1.30","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.259590"},{"key":"2023110803051181000_2023.11.01.565151v1.31","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201710132"},{"key":"2023110803051181000_2023.11.01.565151v1.32","doi-asserted-by":"publisher","DOI":"10.3892\/or.2019.7361"},{"key":"2023110803051181000_2023.11.01.565151v1.33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000640"},{"key":"2023110803051181000_2023.11.01.565151v1.34","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-31751-0"},{"key":"2023110803051181000_2023.11.01.565151v1.35","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.60"},{"key":"2023110803051181000_2023.11.01.565151v1.36","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2020.08.005"},{"key":"2023110803051181000_2023.11.01.565151v1.37","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2023.1182573"},{"key":"2023110803051181000_2023.11.01.565151v1.38","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201608081"},{"key":"2023110803051181000_2023.11.01.565151v1.39","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2201096119"},{"key":"2023110803051181000_2023.11.01.565151v1.40","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02555"},{"key":"2023110803051181000_2023.11.01.565151v1.41","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2011.06.027"},{"key":"2023110803051181000_2023.11.01.565151v1.42","doi-asserted-by":"publisher","DOI":"10.1051\/medsci\/20031967725"},{"key":"2023110803051181000_2023.11.01.565151v1.43","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)61397-7"},{"key":"2023110803051181000_2023.11.01.565151v1.44","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.118.301532"},{"key":"2023110803051181000_2023.11.01.565151v1.45","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1711979114"},{"key":"2023110803051181000_2023.11.01.565151v1.46","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-1012-2"},{"key":"2023110803051181000_2023.11.01.565151v1.47","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2014.01.002"},{"key":"2023110803051181000_2023.11.01.565151v1.48","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.06.040"},{"key":"2023110803051181000_2023.11.01.565151v1.49","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200707090"},{"key":"2023110803051181000_2023.11.01.565151v1.50","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.07.042"},{"key":"2023110803051181000_2023.11.01.565151v1.51","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-022-12142-3"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2023.11.01.565151","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T05:28:29Z","timestamp":1768454909000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2023.11.01.565151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,3]]},"references-count":51,"URL":"https:\/\/doi.org\/10.1101\/2023.11.01.565151","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1016\/j.cub.2024.05.015","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2023,11,3]]},"subtype":"preprint"}}