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Within ensembles, transcriptomic alterations in the PFC and NAcore underlie the learning and persistence of cocaine- and sucrose-seeking behavior. However, transcriptomes exclusively driving cocaine seeking independent from sucrose seeking have not yet been defined using a within-subject approach. Using Ai14:cFos-TRAP2 transgenic mice in a dual cocaine and sucrose self-administration model, we fluorescently sorted (FACS) and characterized (RNAseq) the transcriptomes defining cocaine- and sucrose-seeking ensembles. We found reward- and region-specific transcriptomic changes that will help develop clinically relevant genetic approaches to decrease cocaine-seeking behavior without altering non-drug reward-based positive reinforcement.<\/jats:p>","DOI":"10.1101\/2023.11.02.565378","type":"posted-content","created":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T15:45:34Z","timestamp":1699199134000},"source":"Crossref","is-referenced-by-count":2,"title":["Differential genetic expression within reward-specific ensembles in mice"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9291-0104","authenticated-orcid":false,"given":"Carl G.","family":"Litif","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Levi T.","family":"Flom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn L.","family":"Sandum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Skylar L.","family":"Hodgins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucio","family":"Vaccaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry A.","family":"Stitzel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas A.","family":"Blouin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Constanza","family":"Mannino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8805-4561","authenticated-orcid":false,"given":"Jason P.","family":"Gigley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0017-0942","authenticated-orcid":false,"given":"Todd A.","family":"Schoborg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4273-1237","authenticated-orcid":false,"given":"Ana-Clara","family":"Bobadilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024021202401431000_2023.11.02.565378v2.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0893-133X(00)00195-0"},{"key":"2024021202401431000_2023.11.02.565378v2.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2014.11.005"},{"key":"2024021202401431000_2023.11.02.565378v2.3","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apha.2020.09.006"},{"key":"2024021202401431000_2023.11.02.565378v2.4","doi-asserted-by":"crossref","first-page":"e65764","DOI":"10.7554\/eLife.65764","article-title":"Specialized coding patterns among dorsomedial prefrontal neuronal ensembles predict conditioned reward seeking","volume":"10","year":"2021","journal-title":"eLife"},{"key":"2024021202401431000_2023.11.02.565378v2.5","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1496-19.2019"},{"key":"2024021202401431000_2023.11.02.565378v2.6","unstructured":"Hebb DO , The Organization of Behavior: A Neuropsychological Theory (John Wiley & Sons, Inc., 1949)."},{"key":"2024021202401431000_2023.11.02.565378v2.7","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12943"},{"key":"2024021202401431000_2023.11.02.565378v2.8","doi-asserted-by":"crossref","first-page":"3150","DOI":"10.1038\/s41380-020-00888-z","article-title":"Cocaine and sucrose rewards recruit different seeking ensembles in the nucleus accumbens core","volume":"25","year":"2020","journal-title":"Mol. 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