{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:35:23Z","timestamp":1768509323981,"version":"3.49.0"},"posted":{"date-parts":[[2023,11,3]]},"group-title":"Microbiology","reference-count":74,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,5,15]]},"abstract":"<jats:title>Abstracts<\/jats:title>\n                <jats:p>\n                  The parasite\n                  <jats:italic>Toxoplasma gondii<\/jats:italic>\n                  persists in its hosts by converting from replicating tachyzoites to latent bradyzoites housed in tissue cysts. The molecular mechanisms that mediate\n                  <jats:italic>T. gondii<\/jats:italic>\n                  differentiation remain poorly understood. Through a mutagenesis screen, we identified translation initiation factor eIF1.2 as a critical factor for\n                  <jats:italic>T. gondii<\/jats:italic>\n                  differentiation. A F97L mutation in eIF1.2 or the genetic ablation of\n                  <jats:italic>eIF1.2<\/jats:italic>\n                  (\u0394\n                  <jats:italic>eif1.2<\/jats:italic>\n                  ) markedly impeded bradyzoite cyst formation\n                  <jats:italic>in vitro<\/jats:italic>\n                  and\n                  <jats:italic>in vivo<\/jats:italic>\n                  . We demonstrated, at single-molecule level, that the eIF1.2 F97L mutation impacts the scanning process of the ribosome preinitiation complex on a model mRNA. RNA sequencing and ribosome profiling experiments unveiled that \u0394\n                  <jats:italic>eif1.2<\/jats:italic>\n                  parasites are defective in upregulating bradyzoite induction factors BFD1 and BFD2 during stress-induced differentiation. Forced expression of BFD1 or BFD2 significantly restored differentiation in \u0394\n                  <jats:italic>eif1.2<\/jats:italic>\n                  parasites. Together, our findings suggest that eIF1.2 functions by regulating the translation of key differentiation factors necessary to establish chronic toxoplasmosis.\n                <\/jats:p>","DOI":"10.1101\/2023.11.03.565545","type":"posted-content","created":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T23:55:21Z","timestamp":1699055721000},"source":"Crossref","is-referenced-by-count":2,"title":["Translation initiation factor eIF1.2 promotes\n                  <i>Toxoplasma<\/i>\n                  stage conversion by regulating levels of key differentiation factors"],"prefix":"10.64898","author":[{"given":"Fengrong","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Holmes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hea Jin","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pariyamon","family":"Thaprawat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geetha","family":"Kannan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"My-Hang","family":"Huynh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracey L.","family":"Schultz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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