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To overcome such difficulties and obtain more robust estimates of tissue mechanical properties, we describe an AFM force mapping and data analysis pipeline to characterize the mechanical properties of cryosectioned soft tissues. We assessed this approach on mouse optic nerve head and rat trabecular meshwork, cornea, and sclera. Our data show that the use of repeated measurements, outlier exclusion, and log-normal data transformation increases confidence in AFM mechanical measurements, and we propose that this methodology can be broadly applied to measuring soft tissue properties from cryosections.<\/jats:p>","DOI":"10.1101\/2023.11.08.566263","type":"posted-content","created":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T19:20:22Z","timestamp":1699903222000},"source":"Crossref","is-referenced-by-count":1,"title":["A Method for Analyzing AFM Force Mapping Data Obtained from Soft Tissue Cryosections"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9381-5847","authenticated-orcid":false,"given":"Cydney A.","family":"Wong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6148-0429","authenticated-orcid":false,"given":"Nina Sara","family":"Fraticelli Guzm\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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