{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:56:54Z","timestamp":1768507014323,"version":"3.49.0"},"posted":{"date-parts":[[2023,11,13]]},"group-title":"Physiology","reference-count":74,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,11,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Immune dysfunction is one of the central components in the development and progression of endometriosis by establishing a chronic inflammatory environment. Western-style high-fat diets (HFD) have been linked to greater systemic inflammation to cause metabolic and chronic inflammatory diseases, and are also considered an environmental risk factor for gynecologic diseases. Here, we aimed to examine how HFD alter an inflammatory environment in endometriosis and discern their contribution to endometriotic-associated hyperalgesia. Our results showed that HFD-induced obesity enhanced abdominal mechanical allodynia that was induced by endometriotic lesions. Peritoneal inflammatory macrophages and cytokine levels increased by lesion induction were elevated by chronic exposure to HFD. Pain-related mediators in the dorsal root ganglia were further stimulated after lesion induction under the HFD condition. Although HFD did not affect inflammatory macrophages in the peritoneal cavity without lesion induction, the diversity and composition of the gut microbiota were clearly altered by HFD as a sign of low-grade systemic inflammation. Thus, HFD alone might not establish a local inflammatory environment in the pelvic cavity, but it can contribute to further enhancing chronic inflammation, leading to the exacerbation of endometriosis-associated abdominal hyperalgesia following the establishment and progression of the disease.<\/jats:p>","DOI":"10.1101\/2023.11.09.566474","type":"posted-content","created":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T18:35:12Z","timestamp":1699900512000},"source":"Crossref","is-referenced-by-count":4,"title":["High-fat diets promote peritoneal inflammation and augment endometriosis-associated abdominal hyperalgesia"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9580-7648","authenticated-orcid":false,"given":"Tristin","family":"Herup-Wheeler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3390-8827","authenticated-orcid":false,"given":"Mingxin","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6257-2918","authenticated-orcid":false,"given":"Madeleine E.","family":"Harvey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7455-4423","authenticated-orcid":false,"given":"Chandni","family":"Talwar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0403-0971","authenticated-orcid":false,"given":"Ramakrishna","family":"Kommagani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3061-150X","authenticated-orcid":false,"suffix":"II","given":"James A.","family":"MacLean","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5028-799X","authenticated-orcid":false,"given":"Kanako","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023111521101434000_2023.11.09.566474v1.1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1810764"},{"key":"2023111521101434000_2023.11.09.566474v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2018.06.001"},{"key":"2023111521101434000_2023.11.09.566474v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.05.090"},{"issue":"1","key":"2023111521101434000_2023.11.09.566474v1.4","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41572-018-0008-5","article-title":"Endometriosis","volume":"4","year":"2018","journal-title":"Nat Rev Dis Primers"},{"issue":"2","key":"2023111521101434000_2023.11.09.566474v1.5","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.ajog.2019.02.033","article-title":"Assessing research gaps and unmet needs in endometriosis","volume":"221","year":"2019","journal-title":"Am J Obstet Gynecol"},{"key":"2023111521101434000_2023.11.09.566474v1.6","doi-asserted-by":"crossref","first-page":"405","DOI":"10.2147\/IJWH.S207373","article-title":"Rethinking endometriosis care: applying the chronic care model via a multidisciplinary program for the care of women with endometriosis","volume":"11","year":"2019","journal-title":"Int J Womens Health"},{"key":"2023111521101434000_2023.11.09.566474v1.7","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dev335"},{"issue":"2","key":"2023111521101434000_2023.11.09.566474v1.8","first-page":"93","article-title":"Metastatic or Embolic Endometriosis, due to the Menstrual Dissemination of Endometrial Tissue into the Venous Circulation","volume":"3","year":"1927","journal-title":"The American journal of pathology"},{"issue":"2","key":"2023111521101434000_2023.11.09.566474v1.9","first-page":"151","article-title":"Retrograde menstruation in healthy women and in patients with endometriosis","volume":"64","year":"1984","journal-title":"Obstetrics and gynecology"},{"key":"2023111521101434000_2023.11.09.566474v1.10","doi-asserted-by":"publisher","DOI":"10.1210\/er.2018-00242"},{"key":"2023111521101434000_2023.11.09.566474v1.11","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2018.7270"},{"key":"2023111521101434000_2023.11.09.566474v1.12","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01607"},{"issue":"4","key":"2023111521101434000_2023.11.09.566474v1.13","doi-asserted-by":"crossref","first-page":"461","DOI":"10.5114\/ceji.2020.103418","article-title":"Obesity and chronic inflammation crosslinking","volume":"45","year":"2020","journal-title":"Cent Eur J Immunol"},{"key":"2023111521101434000_2023.11.09.566474v1.14","doi-asserted-by":"crossref","unstructured":"Malesza IJ , Malesza M , Walkowiak J , Mussin N , Walkowiak D , Aringazina R , et al. 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