{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:31:02Z","timestamp":1768509062592,"version":"3.49.0"},"posted":{"date-parts":[[2023,11,16]]},"group-title":"Neuroscience","reference-count":68,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,12,17]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Cardiovagal neurons (CVNs) innervate cardiac ganglia through the vagus nerve to control cardiac function. Although the cardioinhibitory role of CVNs in nucleus ambiguus (CVN\n                  <jats:sup>NA<\/jats:sup>\n                  ) is well established, the nature and functionality of CVNs in dorsal motor nucleus of the vagus (CVN\n                  <jats:sup>DMV<\/jats:sup>\n                  ) is less clear. We therefore aimed to characterize CVN\n                  <jats:sup>DMV<\/jats:sup>\n                  anatomically, physiologically, and functionally. Optogenetically activating cholinergic DMV neurons resulted in robust bradycardia through peripheral muscarinic (parasympathetic) and nicotinic (ganglionic) acetylcholine receptors, but not beta-1-adrenergic (sympathetic) receptors. Retrograde tracing from the cardiac fat pad labeled CVN\n                  <jats:sup>NA<\/jats:sup>\n                  and CVN\n                  <jats:sup>DMV<\/jats:sup>\n                  through the vagus nerve. Using whole cell patch clamp, CVN\n                  <jats:sup>DMV<\/jats:sup>\n                  demonstrated greater hyperexcitability and spontaneous action potential firing\n                  <jats:italic>ex vivo<\/jats:italic>\n                  despite similar resting membrane potentials, compared to CVN\n                  <jats:sup>NA<\/jats:sup>\n                  . Chemogenetically activating DMV also caused significant bradycardia with a correlated reduction in anxiety-like behavior. Thus, DMV contains uniquely hyperexcitable CVNs capable of cardioinhibition and robust anxiolysis.\n                <\/jats:p>","DOI":"10.1101\/2023.11.14.566855","type":"posted-content","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T21:05:19Z","timestamp":1700168719000},"source":"Crossref","is-referenced-by-count":0,"title":["Dorsal Motor Vagal Neurons Can Elicit Bradycardia and Reduce Anxiety-Like Behavior"],"prefix":"10.64898","author":[{"given":"Misty M.","family":"Strain","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J.","family":"Conley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lily S.","family":"Kauffman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liliana","family":"Espinoza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Fedorchak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia Castro","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maisie E.","family":"Crook","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maira","family":"Jalil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgia E.","family":"Hodes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen B. 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