{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T16:10:45Z","timestamp":1784736645053,"version":"3.55.0"},"posted":{"date-parts":[[2023,12,1]]},"group-title":"Neuroscience","reference-count":56,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,12,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Drug craving triggered by cues that were once associated with drug intoxication is a major contributor to continued drug-seeking behaviors. Addictive drugs engage molecular pathways of associative learning and memory. Reactivated memories are vulnerable to disruption by interference with the process of reconsolidation, hence targeting reconsolidation could be a strategy to reduce cue-induced drug craving and relapse. Here we examined the circuitry of cocaine contextual memory reconsolidation and explored neuroplasticity following memory reactivation. Mice underwent chemogenetic inhibition of either nucleus accumbens (NA) neurons or the glutamatergic projection neurons from the ventral hippocampus (vHPC) to NA using inhibitory designer receptors exclusively activated by designer drugs (iDREADD). Mice underwent cocaine conditioned place preference followed by reactivation of the cocaine contextual memory. Clozapine-N-oxide (CNO) was administered after memory reactivation to inhibit either NA neurons or the accumbens\u2013projecting vHPC neurons during the reconsolidation period. When retested 3 days later, a significant reduction in the previously established preference for the cocaine context was found in both conditions. FosTRAP2-Ai14 mice were used to identify neurons activated by cocaine memory recall and to evaluate plasticity in NA medium spiny neurons (MSNs) and vHPC pyramidal neurons upon recall of cocaine memories. Results indicate a significant increase in dendritic spine density in NA MSNs activated by cocaine memory recall, particularly of the thin spine type. Sholl analysis indicated longer dendritic length and more branching of NA MSNs after cocaine memory recall than without memory reactivation. vHPC neurons showed increased spine density, with the most robust change in stubby spines. These results implicate a circuit involving glutamatergic projections from the vHPC onto NA neurons which is necessary for the reconsolidation of cocaine memories. Interruption of cocaine memory reconsolidation reduced drug-seeking behavior.<\/jats:p>","DOI":"10.1101\/2023.11.29.569314","type":"posted-content","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T05:55:25Z","timestamp":1701410125000},"source":"Crossref","is-referenced-by-count":10,"title":["The ventral hippocampus and nucleus accumbens as neural substrates for cocaine contextual memory reconsolidation"],"prefix":"10.64898","author":[{"given":"Carolina Caban","family":"Rivera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael","family":"Price","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo P.","family":"Fortuna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chau","family":"Do","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"Shinkle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco G.","family":"Ghilotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangdang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn G.","family":"Kirby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George M.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen M.","family":"Unterwald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2023120412451308000_2023.11.29.569314v1.1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02245266"},{"key":"2023120412451308000_2023.11.29.569314v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.08.006"},{"key":"2023120412451308000_2023.11.29.569314v1.3","doi-asserted-by":"publisher","DOI":"10.1126\/science.572083"},{"key":"2023120412451308000_2023.11.29.569314v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-4328(96)00153-2"},{"key":"2023120412451308000_2023.11.29.569314v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2012.02.004"},{"key":"2023120412451308000_2023.11.29.569314v1.6","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2013.00118"},{"key":"2023120412451308000_2023.11.29.569314v1.7","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-013-3039-3"},{"key":"2023120412451308000_2023.11.29.569314v1.8","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.jbtep.2015.09.012","article-title":"Reactivating addiction-related memories under propranolol to reduce craving: A pilot randomized controlled trial","volume":"50","year":"2016","journal-title":"J Behav Ther Exp Psychiatry"},{"key":"2023120412451308000_2023.11.29.569314v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2017.09.003"},{"issue":"3","key":"2023120412451308000_2023.11.29.569314v1.10","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1001\/jamapsychiatry.2016.3148","article-title":"Effect of a Brief Memory Updating Intervention on Smoking Behavior: A Randomized Clinical Trial","volume":"74","year":"2017","journal-title":"JAMA psychiatry"},{"key":"2023120412451308000_2023.11.29.569314v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2012.01.002"},{"key":"2023120412451308000_2023.11.29.569314v1.12","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-014-3797-6"},{"key":"2023120412451308000_2023.11.29.569314v1.13","doi-asserted-by":"publisher","DOI":"10.1126\/science.1215070"},{"key":"2023120412451308000_2023.11.29.569314v1.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.nlm.2013.06.021"},{"key":"2023120412451308000_2023.11.29.569314v1.15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb06738.x"},{"key":"2023120412451308000_2023.11.29.569314v1.16","doi-asserted-by":"crossref","first-page":"643230","DOI":"10.3389\/fncir.2021.643230","article-title":"Whole-Brain Mapping the Direct Inputs of Dorsal and Ventral CA1 Projection Neurons","volume":"15","year":"2021","journal-title":"Front Neural Circuits"},{"key":"2023120412451308000_2023.11.29.569314v1.17","doi-asserted-by":"crossref","unstructured":"Zhou, Y. , et al., The Projection From Ventral CA1, Not Prefrontal Cortex, to Nucleus Accumbens Core Mediates Recent Memory Retrieval of Cocaine-Conditioned Place Preference. 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