{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T16:58:38Z","timestamp":1780419518799,"version":"3.54.1"},"posted":{"date-parts":[[2023,12,2]]},"group-title":"Biochemistry","reference-count":92,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,12,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                <jats:p>\n                  Osteomyelitis occurs when\n                  <jats:italic>Staphylococcus aureus<\/jats:italic>\n                  invades the bone microenvironment, resulting in a bone marrow abscess with a spatially defined architecture of cells and biomolecules. Imaging mass spectrometry and microscopy are invaluable tools that can be employed to interrogate the lipidome of\n                  <jats:italic>S. aureus<\/jats:italic>\n                  -infected murine femurs to reveal metabolic and signaling consequences of infection. Here, nearly 250 lipids were spatially mapped to healthy and infection-associated morphological features throughout the femur, establishing composition profiles for tissue types. Ether lipids and arachidonoyl lipids were significantly altered between cells and tissue structures in abscesses, suggesting their roles in abscess formation and inflammatory signaling. Sterols, triglycerides, bis(monoacylglycero)phosphates, and gangliosides possessed ring-like distributions throughout the abscess, indicating dysregulated lipid metabolism in a subpopulation of leukocytes that cannot be discerned with traditional microscopy. These data provide chemical insight into the signaling function and metabolism of cells in the fibrotic border of abscesses, likely characteristic of lipid-laden macrophages.\n                <\/jats:p>","DOI":"10.1101\/2023.12.01.569690","type":"posted-content","created":{"date-parts":[[2023,12,2]],"date-time":"2023-12-02T22:40:12Z","timestamp":1701556812000},"source":"Crossref","is-referenced-by-count":4,"title":["<i>In situ<\/i>\n                  lipidomics of\n                  <i>Staphylococcus aureus<\/i>\n                  osteomyelitis using imaging mass spectrometry"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4900-274X","authenticated-orcid":false,"given":"Christopher J.","family":"Good","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8236-7281","authenticated-orcid":false,"given":"Casey E.","family":"Butrico","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9515-3493","authenticated-orcid":false,"given":"Madeline E.","family":"Colley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1846-1580","authenticated-orcid":false,"given":"Katherine N.","family":"Gibson-Corley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1199-1167","authenticated-orcid":false,"given":"James E.","family":"Cassat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9198-5498","authenticated-orcid":false,"given":"Jeffrey M.","family":"Spraggins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5859-3310","authenticated-orcid":false,"given":"Richard M.","family":"Caprioli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2023120603101053000_2023.12.01.569690v1.1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.638085"},{"key":"2023120603101053000_2023.12.01.569690v1.2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD004439.pub3"},{"key":"2023120603101053000_2023.12.01.569690v1.3","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.N.01350"},{"key":"2023120603101053000_2023.12.01.569690v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16727-5"},{"key":"2023120603101053000_2023.12.01.569690v1.5","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00084-17"},{"key":"2023120603101053000_2023.12.01.569690v1.6","doi-asserted-by":"publisher","DOI":"10.3238\/arztebl.2012.0257"},{"key":"2023120603101053000_2023.12.01.569690v1.7","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199808203390806"},{"key":"2023120603101053000_2023.12.01.569690v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2011.01.007"},{"key":"2023120603101053000_2023.12.01.569690v1.9","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.9.1067"},{"key":"2023120603101053000_2023.12.01.569690v1.10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07576.x"},{"key":"2023120603101053000_2023.12.01.569690v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2012.05.008"},{"key":"2023120603101053000_2023.12.01.569690v1.12","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-017-0423-5"},{"key":"2023120603101053000_2023.12.01.569690v1.13","doi-asserted-by":"publisher","DOI":"10.3390\/metabo11010041"},{"key":"2023120603101053000_2023.12.01.569690v1.14","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.13465"},{"key":"2023120603101053000_2023.12.01.569690v1.15","doi-asserted-by":"publisher","DOI":"10.1016\/0009-9120(90)90255-S"},{"key":"2023120603101053000_2023.12.01.569690v1.16","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00780-4"},{"key":"2023120603101053000_2023.12.01.569690v1.17","doi-asserted-by":"publisher","DOI":"10.1021\/ac970888i"},{"key":"2023120603101053000_2023.12.01.569690v1.18","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1094"},{"key":"2023120603101053000_2023.12.01.569690v1.19","doi-asserted-by":"publisher","DOI":"10.1021\/ac0351264"},{"key":"2023120603101053000_2023.12.01.569690v1.20","doi-asserted-by":"publisher","DOI":"10.1021\/ac502170f"},{"key":"2023120603101053000_2023.12.01.569690v1.21","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b02884"},{"key":"2023120603101053000_2023.12.01.569690v1.22","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3296"},{"key":"2023120603101053000_2023.12.01.569690v1.23","doi-asserted-by":"publisher","DOI":"10.1126\/science.1152449"},{"key":"2023120603101053000_2023.12.01.569690v1.24","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.04.018"},{"key":"2023120603101053000_2023.12.01.569690v1.25","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.8b05050"},{"key":"2023120603101053000_2023.12.01.569690v1.26","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aan6361"},{"key":"2023120603101053000_2023.12.01.569690v1.27","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1913991116"},{"key":"2023120603101053000_2023.12.01.569690v1.28","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2022.05.004"},{"key":"2023120603101053000_2023.12.01.569690v1.29","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.2c00370"},{"key":"2023120603101053000_2023.12.01.569690v1.30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003068"},{"key":"2023120603101053000_2023.12.01.569690v1.31","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201300046"},{"key":"2023120603101053000_2023.12.01.569690v1.32","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4174"},{"key":"2023120603101053000_2023.12.01.569690v1.33","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109683"},{"key":"2023120603101053000_2023.12.01.569690v1.34","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2014.08.024"},{"key":"2023120603101053000_2023.12.01.569690v1.35","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b00985"},{"key":"2023120603101053000_2023.12.01.569690v1.36","doi-asserted-by":"publisher","DOI":"10.1038\/srep41926"},{"key":"2023120603101053000_2023.12.01.569690v1.37","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c02566"},{"key":"2023120603101053000_2023.12.01.569690v1.38","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c04604"},{"key":"2023120603101053000_2023.12.01.569690v1.39","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.05.003"},{"key":"2023120603101053000_2023.12.01.569690v1.40","doi-asserted-by":"publisher","DOI":"10.1002\/jor.24170"},{"key":"2023120603101053000_2023.12.01.569690v1.41","doi-asserted-by":"publisher","DOI":"10.1128\/jb.94.6.1854-1867.1967"},{"key":"2023120603101053000_2023.12.01.569690v1.42","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.5b00915"},{"key":"2023120603101053000_2023.12.01.569690v1.43","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00339-20"},{"key":"2023120603101053000_2023.12.01.569690v1.44","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3322"},{"key":"2023120603101053000_2023.12.01.569690v1.45","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007744"},{"key":"2023120603101053000_2023.12.01.569690v1.46","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-020-02920-1"},{"key":"2023120603101053000_2023.12.01.569690v1.47","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2022.08.061"},{"key":"2023120603101053000_2023.12.01.569690v1.48","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)37812-3"},{"key":"2023120603101053000_2023.12.01.569690v1.49","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D003715"},{"key":"2023120603101053000_2023.12.01.569690v1.50","doi-asserted-by":"publisher","DOI":"10.1159\/000489406"},{"key":"2023120603101053000_2023.12.01.569690v1.51","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015587"},{"key":"2023120603101053000_2023.12.01.569690v1.52","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.50.33203"},{"key":"2023120603101053000_2023.12.01.569690v1.53","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01263.x"},{"key":"2023120603101053000_2023.12.01.569690v1.54","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400404"},{"key":"2023120603101053000_2023.12.01.569690v1.55","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1801653"},{"key":"2023120603101053000_2023.12.01.569690v1.56","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1200189109"},{"key":"2023120603101053000_2023.12.01.569690v1.57","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1712887114"},{"key":"2023120603101053000_2023.12.01.569690v1.58","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101029"},{"key":"2023120603101053000_2023.12.01.569690v1.59","doi-asserted-by":"publisher","DOI":"10.1111\/imm.13449"},{"key":"2023120603101053000_2023.12.01.569690v1.60","doi-asserted-by":"publisher","DOI":"10.2310\/JIM.0b013e31819aaa76"},{"key":"2023120603101053000_2023.12.01.569690v1.61","doi-asserted-by":"publisher","DOI":"10.3390\/cells8080799"},{"key":"2023120603101053000_2023.12.01.569690v1.62","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2019.10.002"},{"key":"2023120603101053000_2023.12.01.569690v1.63","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0085-z"},{"key":"2023120603101053000_2023.12.01.569690v1.64","doi-asserted-by":"publisher","DOI":"10.1101\/2023.06.08.542766"},{"key":"2023120603101053000_2023.12.01.569690v1.65","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01394"},{"key":"2023120603101053000_2023.12.01.569690v1.66","doi-asserted-by":"publisher","DOI":"10.5551\/jat.10249"},{"key":"2023120603101053000_2023.12.01.569690v1.67","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.5.3087"},{"key":"2023120603101053000_2023.12.01.569690v1.68","doi-asserted-by":"crossref","unstructured":"Denning, N.-L. ; Aziz, M. ; Gurien, S. D. ; Wang, P . DAMPs and NETs in Sepsis. Front. Immunol. 2019, 10.","DOI":"10.3389\/fimmu.2019.02536"},{"key":"2023120603101053000_2023.12.01.569690v1.69","doi-asserted-by":"crossref","unstructured":"Gibson, M. S. ; Domingues, N. ; Vieira, O. V . Lipid and Non-Lipid Factors Affecting Macrophage Dysfunction and Inflammation in Atherosclerosis. Front. Physiol. 2018, 9.","DOI":"10.3389\/fphys.2018.00654"},{"key":"2023120603101053000_2023.12.01.569690v1.70","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17402-1"},{"key":"2023120603101053000_2023.12.01.569690v1.71","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2009.09.006"},{"key":"2023120603101053000_2023.12.01.569690v1.72","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.113.301857"},{"key":"2023120603101053000_2023.12.01.569690v1.73","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(00)00076-4"},{"key":"2023120603101053000_2023.12.01.569690v1.74","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)92023-D"},{"key":"2023120603101053000_2023.12.01.569690v1.75","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413862200"},{"key":"2023120603101053000_2023.12.01.569690v1.76","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M057851"},{"key":"2023120603101053000_2023.12.01.569690v1.77","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2018.01.012"},{"key":"2023120603101053000_2023.12.01.569690v1.78","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900365"},{"key":"2023120603101053000_2023.12.01.569690v1.79","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1836"},{"key":"2023120603101053000_2023.12.01.569690v1.80","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601363"},{"key":"2023120603101053000_2023.12.01.569690v1.81","doi-asserted-by":"publisher","DOI":"10.26434\/chemrxiv-2023-whtvz"},{"key":"2023120603101053000_2023.12.01.569690v1.82","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3181adbcfe"},{"key":"2023120603101053000_2023.12.01.569690v1.83","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-989-5_11"},{"key":"2023120603101053000_2023.12.01.569690v1.84","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b03612"},{"key":"2023120603101053000_2023.12.01.569690v1.85","doi-asserted-by":"publisher","DOI":"10.2485\/jhtb.30.165"},{"key":"2023120603101053000_2023.12.01.569690v1.86","unstructured":"Colley, M. E. ; Allen, J. ; Djambazova, K. V. ; Kruse, A. ; melissa.a.farrow; Spraggins, J. Bulk Untargeted LC-MS\/MS Lipidomics. 2023."},{"key":"2023120603101053000_2023.12.01.569690v1.87","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D700041-JLR200"},{"key":"2023120603101053000_2023.12.01.569690v1.88","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-14044-x"},{"key":"2023120603101053000_2023.12.01.569690v1.89","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0531-2"},{"key":"2023120603101053000_2023.12.01.569690v1.90","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl838"},{"key":"2023120603101053000_2023.12.01.569690v1.91","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.S120001025"},{"key":"2023120603101053000_2023.12.01.569690v1.92","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-016-1579-6"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2023.12.01.569690","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T05:36:20Z","timestamp":1768455380000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2023.12.01.569690"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,2]]},"references-count":92,"URL":"https:\/\/doi.org\/10.1101\/2023.12.01.569690","relation":{},"subject":[],"published":{"date-parts":[[2023,12,2]]},"subtype":"preprint"}}