{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:33:04Z","timestamp":1768509184561,"version":"3.49.0"},"posted":{"date-parts":[[2023,12,10]]},"group-title":"Neuroscience","reference-count":31,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,12,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The Wnt pathway plays critical roles in neurogenesis. The expression of\n                  <jats:italic>Axin2<\/jats:italic>\n                  is induced by Wnt\/\u03b2-catenin signaling, making this gene a sensitive indicator of canonical Wnt activity. We employed pulse-chase genetic lineage tracing with the\n                  <jats:italic>Axin2-CreERT2<\/jats:italic>\n                  allele to follow the fate of\n                  <jats:italic>Axin2<\/jats:italic>\n                  -positive cells in the adult hippocampal formation. We found\n                  <jats:italic>Axin2<\/jats:italic>\n                  expressed in astrocytes, neurons and endothelial cells, as well as in the choroid plexus epithelia. Simultaneously with tamoxifen induction of\n                  <jats:italic>Axin2<\/jats:italic>\n                  fate mapping, the dividing cells were marked with 5-ethynyl-2\u2019-deoxyuridine (EdU). Tamoxifen induction resulted in significant increase of dentate gyrus granule cells three months later; however, none of these neurons contained EdU signal. Conversely, six months after the tamoxifen\/EdU pulse-chase labeling, EdU-positive granule neurons were identified in each animal. Our data imply that\n                  <jats:italic>Axin2<\/jats:italic>\n                  is expressed at several different stages of adult granule neuron differentiation and suggest that the process of integration of the adult-born neurons from certain cell lineages may take longer than previously thought.\n                <\/jats:p>","DOI":"10.1101\/2023.12.09.570930","type":"posted-content","created":{"date-parts":[[2023,12,10]],"date-time":"2023-12-10T07:05:16Z","timestamp":1702191916000},"source":"Crossref","is-referenced-by-count":0,"title":["Dynamics of Adult\n                  <i>Axin2<\/i>\n                  Cell Lineage Integration in Granule Neurons of the Dentate Gyrus"],"prefix":"10.64898","author":[{"given":"Khadijeh A.","family":"Sharifi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faraz","family":"Farzad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sauson","family":"Soldozy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0237-2102","authenticated-orcid":false,"given":"Richard J.","family":"Price","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Yashar S.","family":"Kalani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8750-9672","authenticated-orcid":false,"given":"Petr","family":"Tvrdik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023121304550785000_2023.12.09.570930v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-021-00433-z"},{"key":"2023121304550785000_2023.12.09.570930v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2018.04.004"},{"key":"2023121304550785000_2023.12.09.570930v1.3","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00055.2003"},{"key":"2023121304550785000_2023.12.09.570930v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2019.112112"},{"key":"2023121304550785000_2023.12.09.570930v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.02.019"},{"key":"2023121304550785000_2023.12.09.570930v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-022-01176-5"},{"key":"2023121304550785000_2023.12.09.570930v1.7","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1665-19.2020"},{"key":"2023121304550785000_2023.12.09.570930v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(04)00111-4"},{"key":"2023121304550785000_2023.12.09.570930v1.9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.051508.135600"},{"key":"2023121304550785000_2023.12.09.570930v1.10","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1686208"},{"key":"2023121304550785000_2023.12.09.570930v1.11","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-014-1996-4"},{"key":"2023121304550785000_2023.12.09.570930v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.05.023"},{"key":"2023121304550785000_2023.12.09.570930v1.13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1305411110"},{"key":"2023121304550785000_2023.12.09.570930v1.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2011.03.010"},{"key":"2023121304550785000_2023.12.09.570930v1.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.10.021"},{"key":"2023121304550785000_2023.12.09.570930v1.16","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.07.024"},{"key":"2023121304550785000_2023.12.09.570930v1.17","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3389\/fnins.2020.00075","article-title":"\u201cBrain Structural Plasticity: From Adult Neurogenesis to Immature Neurons","volume":"14","year":"2020","journal-title":"Front Neurosci"},{"key":"2023121304550785000_2023.12.09.570930v1.18","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808616105"},{"key":"2023121304550785000_2023.12.09.570930v1.19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805165106"},{"key":"2023121304550785000_2023.12.09.570930v1.20","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.223750"},{"key":"2023121304550785000_2023.12.09.570930v1.21","doi-asserted-by":"publisher","DOI":"10.1124\/pr.117.013896"},{"key":"2023121304550785000_2023.12.09.570930v1.22","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-018-1815-1"},{"issue":"1","key":"2023121304550785000_2023.12.09.570930v1.23","doi-asserted-by":"crossref","first-page":"19689","DOI":"10.1038\/s41598-019-56156-w","article-title":"\u201cGender-specific effects of transthyretin on neural stem cell fate in the subventricular zone of the adult mouse","volume":"9","year":"2019","journal-title":"Sci Rep"},{"key":"2023121304550785000_2023.12.09.570930v1.24","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09298-4"},{"key":"2023121304550785000_2023.12.09.570930v1.25","doi-asserted-by":"crossref","first-page":"55","DOI":"10.3389\/fcell.2019.00055","article-title":"\u201cDivide or Commit - 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