{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:54:11Z","timestamp":1790751251247,"version":"4.1.0"},"posted":{"date-parts":[[2023,12,14]]},"group-title":"Neuroscience","reference-count":49,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,12,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Learned associations between the rewarding effects of drugs and the context in which they are experienced underlie context-induced relapse. Previous work demonstrates the importance of sparse neuronal populations \u2013 called neuronal ensembles \u2013 in associative learning and cocaine seeking, but it remains unknown whether the encoding vs. retrieval of cocaine-associated memories involves similar or distinct mechanisms of ensemble activation and reactivation in nucleus accumbens (NAc). We use ArcCreER\n                  <jats:sup>T2<\/jats:sup>\n                  mice to establish that mostly distinct NAc ensembles are recruited by initial vs. repeated exposures to cocaine, which are then differentially reactivated and exert distinct effects during cocaine-related memory retrieval. Single-nuclei RNA-sequencing of these ensembles demonstrates predominant recruitment of D1 medium spiny neurons and identifies transcriptional properties that are selective to cocaine-recruited NAc neurons and could explain distinct excitability features. These findings fundamentally advance our understanding of how cocaine drives pathological memory formation during repeated exposures.\n                <\/jats:p>","DOI":"10.1101\/2023.12.13.571585","type":"posted-content","created":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T16:10:12Z","timestamp":1702570212000},"source":"Crossref","is-referenced-by-count":5,"title":["Transcriptional correlates of cocaine-associated learning in striatal ARC ensembles"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3543-2156","authenticated-orcid":false,"given":"Marine","family":"Salery","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1732-1498","authenticated-orcid":false,"given":"Arthur","family":"Godino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu Qing","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John F","family":"Fullard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0240-7608","authenticated-orcid":false,"given":"Romain","family":"Durand-de Cuttoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexa R","family":"LaBanca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0954-2298","authenticated-orcid":false,"given":"Leanne M","family":"Holt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott J","family":"Russo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panos","family":"Roussos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7905-2000","authenticated-orcid":false,"given":"Eric J","family":"Nestler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2023121906550679000_2023.12.13.571585v1.1","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1016\/S2215-0366(16)00104-8","article-title":"Neurobiology of addiction: a neurocircuitry analysis","volume":"3","year":"2016","journal-title":"The Lancet Psychiatry"},{"key":"2023121906550679000_2023.12.13.571585v1.2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.116.012484"},{"key":"2023121906550679000_2023.12.13.571585v1.3","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1821-17.2017"},{"key":"2023121906550679000_2023.12.13.571585v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.01.016"},{"key":"2023121906550679000_2023.12.13.571585v1.5","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1038\/s41380-021-01163-5","article-title":"Key transcription factors mediating cocaine-induced plasticity in the nucleus accumbens","volume":"27","year":"2022","journal-title":"Mol Psychiatry"},{"key":"2023121906550679000_2023.12.13.571585v1.6","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6195-10.2011"},{"key":"2023121906550679000_2023.12.13.571585v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2014.11.005"},{"key":"2023121906550679000_2023.12.13.571585v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pbr.2017.07.013"},{"key":"2023121906550679000_2023.12.13.571585v1.9","doi-asserted-by":"publisher","DOI":"10.1101\/lm.046698.117"},{"key":"2023121906550679000_2023.12.13.571585v1.10","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1016\/j.neuron.2019.10.032","article-title":"Complementary Genetic Targeting and Monosynaptic Input Mapping Reveal Recruitment and Refinement of Distributed Corticostriatal Ensembles by Cocaine","volume":"104","year":"2019","journal-title":"Neuron"},{"key":"2023121906550679000_2023.12.13.571585v1.11","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.1038\/s41593-019-0524-y","article-title":"A ventral CA1 to nucleus accumbens core engram circuit mediates conditioned place preference for cocaine","volume":"22","year":"2019","journal-title":"Nat Neurosci"},{"key":"2023121906550679000_2023.12.13.571585v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apha.2020.09.006"},{"key":"2023121906550679000_2023.12.13.571585v1.13","doi-asserted-by":"publisher","DOI":"10.1126\/science.1164139"},{"key":"2023121906550679000_2023.12.13.571585v1.14","doi-asserted-by":"publisher","DOI":"10.1101\/sqb.2014.79.024901"},{"key":"2023121906550679000_2023.12.13.571585v1.15","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3327-14.2014"},{"key":"2023121906550679000_2023.12.13.571585v1.16","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4250"},{"key":"2023121906550679000_2023.12.13.571585v1.17","doi-asserted-by":"crossref","unstructured":"M. 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