{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:05:46Z","timestamp":1768507546188,"version":"3.49.0"},"posted":{"date-parts":[[2023,12,29]]},"group-title":"Biophysics","reference-count":68,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,7,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Over the past decades, single-molecule and super-resolution microscopy have advanced and represent essential tools for life science research. There is,however, a growing gap between the state-of-the-art and what is accessible to biologists, biochemists, medical researchers or labs with financial constraints. To bridge this gap, we introduce Brick-MIC, a versatile and affordable open-source 3D-printed micro-spectroscopy and imaging platform. Brick-MIC enables the integration of various fluorescence imaging techniques with single-molecule resolution within a single platform and exchange between different modalities within minutes. We here present variants of Brick-MIC that facilitate single-molecule fluorescence detection, fluorescence correlation spectroscopy and super-resolution imaging (STORM and PAINT). Detailed descriptions of the hardware and software components, as well as data analysis routines are provided, to allow non-optics specialist to operate their own Brick-MIC with minimal effort and investments. We foresee that our affordable, flexible, and open-source Brick-MIC platform will be a valuable tool for many laboratories worldwide.<\/jats:p>","DOI":"10.1101\/2023.12.29.573596","type":"posted-content","created":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T22:00:14Z","timestamp":1703887214000},"source":"Crossref","is-referenced-by-count":2,"title":["Single-molecule detection and super-resolution imaging with a portable and adaptable 3D-printed microscopy platform (Brick-MIC)"],"prefix":"10.64898","author":[{"given":"Gabriel G.","family":"Moya Mu\u00f1oz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Brix","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philipp","family":"Klocke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul D.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge R.","family":"Luna Piedra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas D.","family":"Wendler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3791-5277","authenticated-orcid":false,"given":"Eitan","family":"Lerner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niels","family":"Zijlstra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8598-5499","authenticated-orcid":false,"given":"Thorben","family":"Cordes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024071200450507000_2023.12.29.573596v2.1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.55133"},{"key":"2024071200450507000_2023.12.29.573596v2.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2021.12.022"},{"key":"2024071200450507000_2023.12.29.573596v2.3","doi-asserted-by":"crossref","first-page":"222","DOI":"10.5599\/admet.733","article-title":"Biophysical methods in early drug discovery","volume":"7","year":"2019","journal-title":"ADMET DMPK"},{"key":"2024071200450507000_2023.12.29.573596v2.4","doi-asserted-by":"crossref","first-page":"024601","DOI":"10.1088\/1361-6633\/aa8a02","article-title":"Single-molecule techniques in biophysics: a review of the progress in methods and applications","volume":"81","year":"2018","journal-title":"Rep Prog Phys"},{"key":"2024071200450507000_2023.12.29.573596v2.5","doi-asserted-by":"crossref","first-page":"317","DOI":"10.2144\/btn-2020-0057","article-title":"PCR past, present and future","volume":"69","year":"2020","journal-title":"Biotechniques"},{"key":"2024071200450507000_2023.12.29.573596v2.6","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-017-1144-z"},{"key":"2024071200450507000_2023.12.29.573596v2.7","doi-asserted-by":"crossref","unstructured":"K. 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