{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T09:22:08Z","timestamp":1768555328535,"version":"3.49.0"},"posted":{"date-parts":[[2024,1,1]]},"group-title":"Microbiology","reference-count":102,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,3,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  <jats:italic>Vibrio cholerae<\/jats:italic>\n                  , the causative agent of the diarrheal disease cholera, poses an ongoing health threat due to its wide repertoire of horizontally acquired elements (HAEs) and virulence factors. New clinical isolates of the bacterium with improved fitness abilities, often associated with HAEs, frequently emerge. The appropriate control and expression of such genetic elements is critical for the bacteria to thrive in the different environmental niches it occupies. H-NS, the histone-like nucleoid structuring protein, is the best studied xenogeneic silencer of HAEs in gamma-proteobacteria. Although H-NS and other highly abundant nucleoid-associated proteins (NAPs) have been shown to play important roles in regulating HAEs and virulence in model bacteria, we still lack a comprehensive understanding of how different NAPs modulate transcription in\n                  <jats:italic>V. cholerae<\/jats:italic>\n                  . By obtaining genome-wide measurements of protein occupancy and active transcription in a clinical isolate of\n                  <jats:italic>V. cholerae,<\/jats:italic>\n                  harboring recently discovered HAEs encoding for phage defense systems, we show that a lack of H-NS causes a robust increase in the expression of genes found in many HAEs. We further found that TsrA, a protein with partial homology to H-NS, regulates virulence genes primarily through modulation of H-NS activity. We also identified a few sites that are affected by TsrA independently of H-NS, suggesting TsrA may act with diverse regulatory mechanisms. Our results demonstrate how the combinatorial activity of NAPs is employed by a clinical isolate of an important pathogen to regulate recently discovered HAEs.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Importance<\/jats:title>\n                  <jats:p>\n                    New strains of the bacterial pathogen\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    , bearing novel horizontally acquired elements (HAEs), frequently emerge. HAEs provide beneficial traits to the bacterium, such as antibiotic resistance and defense against invading bacteriophages. Xenogeneic silencers are proteins that help bacteria harness new HAEs and silence those HAEs until they are needed. H-NS is the best-studied xenogeneic silencer; it is one of the nucleoid-associated proteins (NAPs) in gamma-proteobacteria and is responsible for the proper regulation of HAEs within the bacterial transcriptional network. We studied the effects of H-NS and other NAPs on the HAEs of a clinical isolate of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    . Importantly, we found that H-NS partners with a small and poorly characterized protein, TsrA, to help domesticate new HAEs involved in bacterial survival and in causing disease. Proper understanding of the regulatory state in emerging isolates of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    will provide improved therapies against new isolates of the pathogen.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2023.12.30.573743","type":"posted-content","created":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T19:15:10Z","timestamp":1704136510000},"source":"Crossref","is-referenced-by-count":0,"title":["Nucleoid-associated proteins shape the global protein occupancy and transcriptional landscape of a clinical isolate of\n                  <i>Vibrio cholerae<\/i>"],"prefix":"10.64898","author":[{"given":"Yulduz","family":"Rakibova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Drew T.","family":"Dunham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0139-1600","authenticated-orcid":false,"given":"Kimberley D.","family":"Seed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5821-4226","authenticated-orcid":false,"given":"P. Lydia","family":"Freddolino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024032707350912000_2023.12.30.573743v2.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15328-7"},{"key":"2024032707350912000_2023.12.30.573743v2.2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003832"},{"key":"2024032707350912000_2023.12.30.573743v2.3","unstructured":"Cholera. [cited 10 Apr 2023]. Available: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/cholera"},{"key":"2024032707350912000_2023.12.30.573743v2.4","doi-asserted-by":"publisher","DOI":"10.1038\/35012500"},{"key":"2024032707350912000_2023.12.30.573743v2.5","doi-asserted-by":"crossref","unstructured":"Waldor MK , Mekalanos JJ . Lysogenic conversion by a filamentous phage encoding cholera toxin. Science. 1996. pp. 1910\u20131914.","DOI":"10.1126\/science.272.5270.1910"},{"key":"2024032707350912000_2023.12.30.573743v2.6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.3134"},{"key":"2024032707350912000_2023.12.30.573743v2.7","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00334-10"},{"key":"2024032707350912000_2023.12.30.573743v2.8","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1146\/annurev-virology-091919-072020","article-title":"Bacteriophage ICP1: A Persistent Predator of Vibrio cholerae","volume":"8","year":"2021","journal-title":"Annu Rev Virol"},{"key":"2024032707350912000_2023.12.30.573743v2.9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006838"},{"key":"2024032707350912000_2023.12.30.573743v2.10","first-page":"e0308821","article-title":"Evolutionary Sweeps of Subviral Parasites and Their Phage Host Bring Unique Parasite Variants and Disappearance of a Phage CRISPR-Cas System","volume":"13","year":"2021","journal-title":"MBio"},{"key":"2024032707350912000_2023.12.30.573743v2.11","doi-asserted-by":"publisher","DOI":"10.1101\/218164"},{"key":"2024032707350912000_2023.12.30.573743v2.12","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.53200"},{"key":"2024032707350912000_2023.12.30.573743v2.13","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac002"},{"key":"2024032707350912000_2023.12.30.573743v2.14","doi-asserted-by":"publisher","DOI":"10.1101\/2023.03.01.530633"},{"key":"2024032707350912000_2023.12.30.573743v2.15","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1038\/s41564-022-01162-4","article-title":"Phage defence by deaminase-mediated depletion of deoxynucleotides in bacteria","volume":"7","year":"2022","journal-title":"Nat Microbiol"},{"key":"2024032707350912000_2023.12.30.573743v2.16","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/s41586-022-04546-y","article-title":"Two defence systems eliminate plasmids from seventh pandemic Vibrio cholerae","volume":"604","year":"2022","journal-title":"Nature"},{"key":"2024032707350912000_2023.12.30.573743v2.17","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1038\/s41586-019-1605-5","article-title":"Cyclic GMP\u2013AMP signalling protects bacteria against viral infection","volume":"574","year":"2019","journal-title":"Nature"},{"key":"2024032707350912000_2023.12.30.573743v2.18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1010250"},{"key":"2024032707350912000_2023.12.30.573743v2.19","doi-asserted-by":"crossref","first-page":"4135","DOI":"10.1093\/molbev\/msab136","article-title":"Xenogeneic Silencing and Bacterial Genome Evolution: Mechanisms for DNA Recognition Imply Multifaceted Roles of Xenogeneic Silencers","volume":"38","year":"2021","journal-title":"Mol Biol Evol"},{"key":"2024032707350912000_2023.12.30.573743v2.20","doi-asserted-by":"publisher","DOI":"10.1126\/science.1128794"},{"key":"2024032707350912000_2023.12.30.573743v2.21","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1080\/21541264.2021.1973865","article-title":"Nucleoid-associated proteins shape chromatin structure and transcriptional regulation across the bacterial kingdom","volume":"12","year":"2021","journal-title":"Transcription"},{"key":"2024032707350912000_2023.12.30.573743v2.22","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1543107"},{"key":"2024032707350912000_2023.12.30.573743v2.23","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky265"},{"key":"2024032707350912000_2023.12.30.573743v2.24","doi-asserted-by":"crossref","unstructured":"Shen BA , Hustmyer CM , Roston D , Wolfe MB , Landick R . Bacterial H-NS contacts DNA at the same irregularly spaced sites in both bridged and hemi-sequestered linear filaments. iScience. 2022;25: 104429.","DOI":"10.1016\/j.isci.2022.104429"},{"key":"2024032707350912000_2023.12.30.573743v2.25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.46.33105"},{"key":"2024032707350912000_2023.12.30.573743v2.26","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(01)01232-9"},{"key":"2024032707350912000_2023.12.30.573743v2.27","doi-asserted-by":"publisher","DOI":"10.15252\/EMBJ.2021108708"},{"key":"2024032707350912000_2023.12.30.573743v2.28","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1156"},{"key":"2024032707350912000_2023.12.30.573743v2.29","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abk0233"},{"key":"2024032707350912000_2023.12.30.573743v2.30","doi-asserted-by":"crossref","first-page":"e0059921","DOI":"10.1128\/jb.00599-21","article-title":"RfaH May Oppose Silencing by H-NS and YmoA Proteins during Transcription Elongation","volume":"204","year":"2022","journal-title":"J Bacteriol"},{"key":"2024032707350912000_2023.12.30.573743v2.31","doi-asserted-by":"crossref","first-page":"3679","DOI":"10.1093\/nar\/gkad088","article-title":"Localized modulation of DNA supercoiling, triggered by the Shigella anti-silencer VirB, is sufficient to relieve H-NS-mediated silencing","volume":"51","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"2024032707350912000_2023.12.30.573743v2.32","doi-asserted-by":"crossref","first-page":"9229","DOI":"10.1093\/nar\/gkab678","article-title":"A phage-encoded nucleoid associated protein compacts both host and phage DNA and derepresses H-NS silencing","volume":"49","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"2024032707350912000_2023.12.30.573743v2.33","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00089-08"},{"key":"2024032707350912000_2023.12.30.573743v2.34","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01343-09"},{"key":"2024032707350912000_2023.12.30.573743v2.35","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.15.4295-4303.2000"},{"key":"2024032707350912000_2023.12.30.573743v2.36","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.4.1301-1314.1998"},{"key":"2024032707350912000_2023.12.30.573743v2.37","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1608732113"},{"key":"2024032707350912000_2023.12.30.573743v2.38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0118295"},{"key":"2024032707350912000_2023.12.30.573743v2.39","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2016.07.007"},{"key":"2024032707350912000_2023.12.30.573743v2.40","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05900-11"},{"key":"2024032707350912000_2023.12.30.573743v2.41","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00564-06"},{"key":"2024032707350912000_2023.12.30.573743v2.42","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.15.4259-4269.2002"},{"key":"2024032707350912000_2023.12.30.573743v2.43","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000786"},{"key":"2024032707350912000_2023.12.30.573743v2.44","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02901-20"},{"key":"2024032707350912000_2023.12.30.573743v2.45","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.01014-20"},{"key":"2024032707350912000_2023.12.30.573743v2.46","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1014998107"},{"key":"2024032707350912000_2023.12.30.573743v2.47","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00358-20"},{"key":"2024032707350912000_2023.12.30.573743v2.48","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv071"},{"key":"2024032707350912000_2023.12.30.573743v2.49","doi-asserted-by":"publisher","DOI":"10.1126\/science.abg2166"},{"key":"2024032707350912000_2023.12.30.573743v2.50","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.06.035"},{"key":"2024032707350912000_2023.12.30.573743v2.51","doi-asserted-by":"crossref","first-page":"e3001557","DOI":"10.1371\/journal.pbio.3001557","article-title":"Correction: Dynamic landscape of protein occupancy across the Escherichia coli chromosome","volume":"20","year":"2022","journal-title":"PLoS Biol"},{"key":"2024032707350912000_2023.12.30.573743v2.52","doi-asserted-by":"publisher","DOI":"10.1038\/nature02241"},{"key":"2024032707350912000_2023.12.30.573743v2.53","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2006.01.001"},{"key":"2024032707350912000_2023.12.30.573743v2.54","doi-asserted-by":"crossref","unstructured":"McKnight SL. Phage \u03bb: A Genetic Switch. Gene Control and Phage \u03bb. Marc Ptashne. Cell Press and Blackwell Scientific, Palo Alto, CA, 1986. x, 128 pp., illus. Paper, $16.95. Science. 1986;233: 1435\u20131436.","DOI":"10.1126\/science.233.4771.1435.b"},{"key":"2024032707350912000_2023.12.30.573743v2.55","doi-asserted-by":"publisher","DOI":"10.1038\/nature11927"},{"key":"2024032707350912000_2023.12.30.573743v2.56","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00518-12"},{"key":"2024032707350912000_2023.12.30.573743v2.57","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00834-06"},{"key":"2024032707350912000_2023.12.30.573743v2.58","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01867-06"},{"key":"2024032707350912000_2023.12.30.573743v2.59","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01957-14"},{"key":"2024032707350912000_2023.12.30.573743v2.60","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00025-14"},{"key":"2024032707350912000_2023.12.30.573743v2.61","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.042667999"},{"key":"2024032707350912000_2023.12.30.573743v2.62","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27172-0"},{"key":"2024032707350912000_2023.12.30.573743v2.63","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.11.016"},{"key":"2024032707350912000_2023.12.30.573743v2.64","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-5928(03)00145-1"},{"key":"2024032707350912000_2023.12.30.573743v2.65","doi-asserted-by":"publisher","DOI":"10.1101\/gr.092759.109"},{"key":"2024032707350912000_2023.12.30.573743v2.66","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003800"},{"key":"2024032707350912000_2023.12.30.573743v2.67","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1107894108"},{"key":"2024032707350912000_2023.12.30.573743v2.68","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki715"},{"key":"2024032707350912000_2023.12.30.573743v2.69","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002126"},{"key":"2024032707350912000_2023.12.30.573743v2.70","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3419-3426.2002"},{"key":"2024032707350912000_2023.12.30.573743v2.71","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005570"},{"key":"2024032707350912000_2023.12.30.573743v2.72","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1111\/mmi.15046","article-title":"The RNA-RNA interactome between a phage and its satellite virus reveals a small RNA that differentially regulates gene expression across both genomes","volume":"119","year":"2023","journal-title":"Mol Microbiol"},{"key":"2024032707350912000_2023.12.30.573743v2.73","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00243-20"},{"key":"2024032707350912000_2023.12.30.573743v2.74","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1100"},{"key":"2024032707350912000_2023.12.30.573743v2.75","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1002\/prot.26585","article-title":"Integrating deep learning, threading alignments, and a multi-MSA strategy for high-quality protein monomer and complex structure prediction in CASP15","volume":"91","year":"2023","journal-title":"Proteins"},{"key":"2024032707350912000_2023.12.30.573743v2.76","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01773-0"},{"key":"2024032707350912000_2023.12.30.573743v2.77","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.1020090107"},{"key":"2024032707350912000_2023.12.30.573743v2.78","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.14.4157-4165.1996"},{"key":"2024032707350912000_2023.12.30.573743v2.79","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00150-08"},{"key":"2024032707350912000_2023.12.30.573743v2.80","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3425"},{"key":"2024032707350912000_2023.12.30.573743v2.81","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2004.07.008"},{"key":"2024032707350912000_2023.12.30.573743v2.82","doi-asserted-by":"publisher","DOI":"10.1007\/s004380051178"},{"key":"2024032707350912000_2023.12.30.573743v2.83","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00773"},{"key":"2024032707350912000_2023.12.30.573743v2.84","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1146\/annurev-virology-031413-085446","article-title":"The Phage-Inducible Chromosomal Islands: A Family of Highly Evolved Molecular Parasites","volume":"2","year":"2015","journal-title":"Annu Rev Virol"},{"key":"2024032707350912000_2023.12.30.573743v2.85","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac855"},{"key":"2024032707350912000_2023.12.30.573743v2.86","doi-asserted-by":"publisher","DOI":"10.1038\/nature09065"},{"key":"2024032707350912000_2023.12.30.573743v2.87","doi-asserted-by":"crossref","first-page":"10770","DOI":"10.1093\/nar\/gkab793","article-title":"Novel anti-repression mechanism of H-NS proteins by a phage protein","volume":"49","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"2024032707350912000_2023.12.30.573743v2.88","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1406478111"},{"key":"2024032707350912000_2023.12.30.573743v2.89","first-page":"10","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet","volume":"17","year":"2011","journal-title":"journal"},{"key":"2024032707350912000_2023.12.30.573743v2.90","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"2024032707350912000_2023.12.30.573743v2.91","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"2024032707350912000_2023.12.30.573743v2.92","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi1112s47"},{"key":"2024032707350912000_2023.12.30.573743v2.93","doi-asserted-by":"crossref","unstructured":"Chen Y , Nie F , Xie S-Q , Zheng Y-F , Dai Q , Bray T , et al. Efficient assembly of nanopore reads via highly accurate and intact error correction. Nat Commun. 2021;12: 60.","DOI":"10.1038\/s41467-020-20236-7"},{"key":"2024032707350912000_2023.12.30.573743v2.94","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112963"},{"key":"2024032707350912000_2023.12.30.573743v2.95","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0554-6_12"},{"key":"2024032707350912000_2023.12.30.573743v2.96","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"2024032707350912000_2023.12.30.573743v2.97","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2018.0089"},{"key":"2024032707350912000_2023.12.30.573743v2.98","doi-asserted-by":"crossref","unstructured":"Kurtz S , Phillippy A , Delcher AL , Smoot M , Shumway M , Antonescu C , et al. Versatile and open software for comparing large genomes. Genome Biol. 2004;5: R12.","DOI":"10.1186\/gb-2004-5-2-r12"},{"key":"2024032707350912000_2023.12.30.573743v2.99","doi-asserted-by":"crossref","unstructured":"Bray NL , Pimentel H , Melsted P , Pachter L . Erratum: Near-optimal probabilistic RNA-seq quantification. Nat Biotechnol. 2016;34: 888.","DOI":"10.1038\/nbt0816-888d"},{"key":"2024032707350912000_2023.12.30.573743v2.100","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4324"},{"key":"2024032707350912000_2023.12.30.573743v2.101","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giab008"},{"key":"2024032707350912000_2023.12.30.573743v2.102","doi-asserted-by":"crossref","first-page":"e1010793","DOI":"10.1371\/journal.pcbi.1010793","article-title":"Integrating unsupervised language model with triplet neural networks for protein gene ontology prediction","volume":"18","year":"2022","journal-title":"PLoS Comput Biol"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2023.12.30.573743","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T05:44:38Z","timestamp":1768455878000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2023.12.30.573743"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,1]]},"references-count":102,"URL":"https:\/\/doi.org\/10.1101\/2023.12.30.573743","relation":{},"subject":[],"published":{"date-parts":[[2024,1,1]]},"subtype":"preprint"}}