{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:00:03Z","timestamp":1782280803231,"version":"3.54.5"},"posted":{"date-parts":[[2024,1,11]]},"group-title":"Developmental Biology","reference-count":72,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,1,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Adherens junctions (AJs) are a fundamental organizing structure for multicellular life. Although AJs are studied mainly in epithelia, their core function \u2013 stabilizing cell contacts by coupling adhesion molecules to the cytoskeleton \u2013 is important in diverse tissues. We find that two\n                  <jats:italic>C. elegans<\/jats:italic>\n                  sensory neurons, URX and BAG, require conserved AJ proteins for dendrite morphogenesis. We previously showed that URX and BAG dendrites attach to the embryonic nose via the adhesion molecule SAX-7\/L1CAM, acting both in neurons and glia, and then extend by stretch during embryo elongation. Here, we find that a PDZ-binding motif (PB) in the SAX-7 cytoplasmic tail acts with other interaction motifs to promote dendrite extension. Using pull-down assays, we find that the SAX-7 PB binds the multi-PDZ scaffolding protein MAGI-1, which bridges it to the cadherin-catenin complex protein HMP-2\/\u03b2-catenin. Using cell-specific rescue and depletion, we find that both MAGI-1 and HMR-1\/Cadherin act in glia to non-autonomously promote dendrite extension. Double mutant analysis indicates that each protein can act independently of SAX-7, suggesting a multivalent adhesion complex. The SAX-7 PB motif also binds AFD-1\/Afadin, loss of which further enhances\n                  <jats:italic>sax-7<\/jats:italic>\n                  BAG dendrite defects. As MAGI-1, HMR-1, and AFD-1 are all found in epithelial AJs, we propose that an AJ-like complex in glia promotes dendrite extension.\n                <\/jats:p>","DOI":"10.1101\/2024.01.11.575259","type":"posted-content","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T04:00:32Z","timestamp":1705032032000},"source":"Crossref","is-referenced-by-count":3,"title":["SAX-7\/L1CAM acts with the adherens junction proteins MAGI-1, HMR-1\/Cadherin, and AFD-1\/Afadin to promote glial-mediated dendrite extension"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5218-265X","authenticated-orcid":false,"given":"Elizabeth R.","family":"Cebul","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0033-396X","authenticated-orcid":false,"given":"Arthur","family":"Marivin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leland R.","family":"Wexler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola N.","family":"Perrat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1919-1877","authenticated-orcid":false,"given":"Claire Y.","family":"B\u00e9nard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9513-4826","authenticated-orcid":false,"given":"Mikel","family":"Garcia-Marcos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2557-6490","authenticated-orcid":false,"given":"Maxwell G.","family":"Heiman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024011320151440000_2024.01.11.575259v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2010.08.002"},{"key":"2024011320151440000_2024.01.11.575259v1.2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-013-1146-1"},{"key":"2024011320151440000_2024.01.11.575259v1.3","doi-asserted-by":"publisher","DOI":"10.1242\/dev.115048"},{"key":"2024011320151440000_2024.01.11.575259v1.4","doi-asserted-by":"publisher","DOI":"10.1534\/g3.112.003830"},{"key":"2024011320151440000_2024.01.11.575259v1.5","doi-asserted-by":"publisher","DOI":"10.1242\/dev.180448"},{"key":"2024011320151440000_2024.01.11.575259v1.6","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200009004"},{"key":"2024011320151440000_2024.01.11.575259v1.7","doi-asserted-by":"publisher","DOI":"10.1242\/dev.079863"},{"key":"2024011320151440000_2024.01.11.575259v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)80024-6"},{"key":"2024011320151440000_2024.01.11.575259v1.9","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.141.1.297"},{"key":"2024011320151440000_2024.01.11.575259v1.10","doi-asserted-by":"crossref","first-page":"iyab086","DOI":"10.1093\/genetics\/iyab086","article-title":"Neuronal postdevelopmentally acting SAX-7S\/L1CAM can function as cleaved fragments to maintain neuronal architecture in Caenorhabditis elegans","volume":"218","year":"2021","journal-title":"Genetics"},{"key":"2024011320151440000_2024.01.11.575259v1.11","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.2471"},{"key":"2024011320151440000_2024.01.11.575259v1.12","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.01948"},{"key":"2024011320151440000_2024.01.11.575259v1.13","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2012-101190"},{"key":"2024011320151440000_2024.01.11.575259v1.14","doi-asserted-by":"publisher","DOI":"10.1159\/000319859"},{"key":"2024011320151440000_2024.01.11.575259v1.15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004613"},{"key":"2024011320151440000_2024.01.11.575259v1.16","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.129.1.189"},{"key":"2024011320151440000_2024.01.11.575259v1.17","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.17.2.375"},{"key":"2024011320151440000_2024.01.11.575259v1.18","doi-asserted-by":"crossref","unstructured":"Fung, W. , Wexler, L. and Heiman, M. G . (2020). Cell-type-specific promoters for C. elegans glia. Journal of Neurogenetics 1\u201312.","DOI":"10.1101\/2020.06.01.128546"},{"key":"2024011320151440000_2024.01.11.575259v1.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.05.507"},{"key":"2024011320151440000_2024.01.11.575259v1.20","doi-asserted-by":"crossref","unstructured":"Hardin, J. , Lynch, A. , Loveless, T. and Pettitt, J . (2013). Cadherins and Their Partners in the Nematode Worm Caenorhabditis elegans. In Progress in Molecular Biology and Translational Science, pp. 239\u2013262. Elsevier.","DOI":"10.1016\/B978-0-12-394311-8.00011-X"},{"key":"2024011320151440000_2024.01.11.575259v1.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2007.07.012"},{"key":"2024011320151440000_2024.01.11.575259v1.22","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.ydbio.2022.06.012","article-title":"When is a neuron like an epithelial cell","volume":"489","year":"2022","journal-title":"Dev Biol"},{"key":"2024011320151440000_2024.01.11.575259v1.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.057"},{"key":"2024011320151440000_2024.01.11.575259v1.24","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122002"},{"key":"2024011320151440000_2024.01.11.575259v1.25","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1186\/1471-2105-11-507","article-title":"Proteome scanning to predict PDZ domain interactions using support vector machines","volume":"11","year":"2010","journal-title":"BMC Bioinformatics"},{"key":"2024011320151440000_2024.01.11.575259v1.26","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-27"},{"key":"2024011320151440000_2024.01.11.575259v1.27","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.146.5.1117"},{"key":"2024011320151440000_2024.01.11.575259v1.28","doi-asserted-by":"publisher","DOI":"10.4161\/tisb.28426"},{"key":"2024011320151440000_2024.01.11.575259v1.29","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-4682(10)80011-X"},{"key":"2024011320151440000_2024.01.11.575259v1.30","doi-asserted-by":"crossref","unstructured":"Labouesse, M. (2006). Epithelial junctions and attachments. WormBook.","DOI":"10.1895\/wormbook.1.56.1"},{"key":"2024011320151440000_2024.01.11.575259v1.31","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2017.09.011"},{"key":"2024011320151440000_2024.01.11.575259v1.32","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1002\/ar.1090230708","article-title":"The adhesive quality of cells","volume":"23","year":"1922","journal-title":"Anat. Rec"},{"key":"2024011320151440000_2024.01.11.575259v1.33","doi-asserted-by":"crossref","first-page":"iyac036","DOI":"10.1093\/genetics\/iyac036","article-title":"Axon-dendrite and apical-basolateral sorting in a single neuron","volume":"221","year":"2022","journal-title":"Genetics"},{"key":"2024011320151440000_2024.01.11.575259v1.34","doi-asserted-by":"publisher","DOI":"10.1242\/dev.171124"},{"key":"2024011320151440000_2024.01.11.575259v1.35","doi-asserted-by":"crossref","unstructured":"Lynch, A. , M. and Hardin, J. (2009). The assembly and maintenance of epithelial junctions in C. elegans. Front Biosci Volume, 1414.","DOI":"10.2741\/3316"},{"key":"2024011320151440000_2024.01.11.575259v1.36","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2012.08.024"},{"key":"2024011320151440000_2024.01.11.575259v1.37","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.2.517"},{"key":"2024011320151440000_2024.01.11.575259v1.38","doi-asserted-by":"crossref","unstructured":"Mandai, K. , Rikitake, Y. , Shimono, Y. and Takai, Y . (2013). Afadin\/AF-6 and Canoe. In Progress in Molecular Biology and Translational Science, pp. 433\u2013454. Elsevier.","DOI":"10.1016\/B978-0-12-394311-8.00019-4"},{"key":"2024011320151440000_2024.01.11.575259v1.39","doi-asserted-by":"crossref","first-page":"1938","DOI":"10.1091\/mbc.E19-04-0209","article-title":"The Drosophila Afadin and ZO-1 homologues Canoe and Polychaetoid act in parallel to maintain epithelial integrity when challenged by adherens junction remodeling","volume":"30","year":"2019","journal-title":"MBoC"},{"key":"2024011320151440000_2024.01.11.575259v1.40","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1002\/wdev.171","article-title":"The many glia of a tiny nematode: studying glial diversity using Caenorhabditis elegans: Glial diversity in C. elegans","volume":"4","year":"2015","journal-title":"WIREs Dev Biol"},{"key":"2024011320151440000_2024.01.11.575259v1.41","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500375200"},{"key":"2024011320151440000_2024.01.11.575259v1.42","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2016.07.013"},{"key":"2024011320151440000_2024.01.11.575259v1.43","doi-asserted-by":"crossref","first-page":"fcac288","DOI":"10.1093\/braincomms\/fcac288","article-title":"Ependymal cells and neurodegenerative disease: outcomes of compromised ependymal barrier function","volume":"4","year":"2022","journal-title":"Brain Communications"},{"key":"2024011320151440000_2024.01.11.575259v1.44","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1120685109"},{"key":"2024011320151440000_2024.01.11.575259v1.45","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700865"},{"key":"2024011320151440000_2024.01.11.575259v1.46","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.bbamem.2007.12.015","article-title":"Molecular components of the adherens junction","volume":"1778","year":"2008","journal-title":"Biochimica et Biophysica Acta (BBA) - Biomembranes"},{"key":"2024011320151440000_2024.01.11.575259v1.47","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.1300314"},{"key":"2024011320151440000_2024.01.11.575259v1.48","doi-asserted-by":"crossref","unstructured":"Pasti, G. and Labouesse, M . (2014). Epithelial junctions, cytoskeleton, and polarity. WormBook 1\u201335.","DOI":"10.1895\/wormbook.1.56.2"},{"key":"2024011320151440000_2024.01.11.575259v1.49","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-142X.2007.00217.x"},{"key":"2024011320151440000_2024.01.11.575259v1.50","doi-asserted-by":"publisher","DOI":"10.4067\/S0716-97602012000300005"},{"key":"2024011320151440000_2024.01.11.575259v1.51","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1002\/ajmg.a.38592","article-title":"Bi-allelic mutations of CCDC88C are a rare cause of severe congenital hydrocephalus","volume":"176","year":"2018","journal-title":"Am J Med Genet"},{"key":"2024011320151440000_2024.01.11.575259v1.52","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201907079"},{"key":"2024011320151440000_2024.01.11.575259v1.53","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200904001"},{"key":"2024011320151440000_2024.01.11.575259v1.54","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2023.1092205"},{"key":"2024011320151440000_2024.01.11.575259v1.55","doi-asserted-by":"crossref","first-page":"e1010507","DOI":"10.1371\/journal.pgen.1010507","article-title":"SRGP-1\/srGAP and AFD-1\/afadin stabilize HMP-1\/lZ-catenin at rosettes to seal internalization sites following gastrulation in C. elegans","volume":"19","year":"2023","journal-title":"PLoS Genet"},{"key":"2024011320151440000_2024.01.11.575259v1.56","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-neuro-070918-050314"},{"key":"2024011320151440000_2024.01.11.575259v1.57","doi-asserted-by":"publisher","DOI":"10.1007\/BF00341419"},{"key":"2024011320151440000_2024.01.11.575259v1.58","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.10.026"},{"key":"2024011320151440000_2024.01.11.575259v1.59","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006019"},{"key":"2024011320151440000_2024.01.11.575259v1.60","doi-asserted-by":"publisher","DOI":"10.1006\/prep.2001.1603"},{"key":"2024011320151440000_2024.01.11.575259v1.61","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M405561200"},{"key":"2024011320151440000_2024.01.11.575259v1.62","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(95)80102-2"},{"key":"2024011320151440000_2024.01.11.575259v1.63","doi-asserted-by":"crossref","first-page":"e202303138","DOI":"10.1083\/jcb.202303138","article-title":"\u03b4-Catenin controls astrocyte morphogenesis via layer-specific astrocyte\u2013 neuron cadherin interactions","volume":"222","year":"2023","journal-title":"Journal of Cell Biology"},{"key":"2024011320151440000_2024.01.11.575259v1.64","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0060239"},{"key":"2024011320151440000_2024.01.11.575259v1.65","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-16-06897.1999"},{"key":"2024011320151440000_2024.01.11.575259v1.66","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2015.03.002"},{"key":"2024011320151440000_2024.01.11.575259v1.67","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1016\/j.celrep.2016.03.051","article-title":"PROS-1\/Prospero Is a Major Regulator of the Glia-Specific Secretome Controlling Sensory-Neuron Shape and Function in C. elegans","volume":"15","year":"2016","journal-title":"Cell Reports"},{"key":"2024011320151440000_2024.01.11.575259v1.68","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901600305"},{"key":"2024011320151440000_2024.01.11.575259v1.69","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080356"},{"key":"2024011320151440000_2024.01.11.575259v1.70","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2011.07.001"},{"key":"2024011320151440000_2024.01.11.575259v1.71","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.108.091272"},{"key":"2024011320151440000_2024.01.11.575259v1.72","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2016.18"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.01.11.575259","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:09:34Z","timestamp":1782277774000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2024.01.11.575259"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,11]]},"references-count":72,"URL":"https:\/\/doi.org\/10.1101\/2024.01.11.575259","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1242\/dev.205242","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2024,1,11]]},"subtype":"preprint"}}