{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T12:30:30Z","timestamp":1776861030404,"version":"3.51.2"},"posted":{"date-parts":[[2024,1,22]]},"group-title":"Neuroscience","reference-count":95,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,1,22]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Aging negatively impacts central nervous system function; however, the cellular impact of aging in the peripheral nervous system remains poorly understood. Aged individuals are more likely to experience increased pain and slower recovery after trauma. Such injury can damage vulnerable peripheral axons of dorsal root ganglion (DRG) neurons resulting in somatosensory dysfunction. One cellular mechanism common to both aging and injury is cellular senescence, a complex cell state that can contribute to the aged pro-inflammatory environment. We uncovered, for the first time, DRG neuron senescence in the context of aging and pain-inducing peripheral nerve injury in young and aged mice. Aged DRG neurons displayed multiple markers of senescence (SA-\u03b2-gal, p21, p16, IL6) when compared to young DRG neurons. Peripheral nerve injury triggered a further accumulation of senescent DRG neurons over time post-injury in young and aged DRG. These senescent neurons were dynamic and heterogeneous in their expression of senescence markers, p16, p21, and senescence-associated secretory phenotype (SASP) expression of IL6, which was influenced by age. An electrophysiological characterization of senescence marker-expressing neurons revealed high-firing and nociceptor-like phenotypes within these populations. In addition, we observed improvement in nociceptive behaviors in young and aged nerve-injured mice after treatment with a senolytic agent that eliminates senescent cells. Finally, we confirmed in human post-mortem DRG samples that neuronal senescence is present and increases with age. Overall, we describe a susceptibility of the peripheral nervous system to neuronal senescence with age or injury that may be a targetable mechanism to treat sensory dysfunction, such as chronic pain, particularly in aged populations.<\/jats:p>","DOI":"10.1101\/2024.01.20.576299","type":"posted-content","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T21:15:22Z","timestamp":1705958122000},"source":"Crossref","is-referenced-by-count":6,"title":["Aging and injury drive neuronal senescence in the dorsal root ganglia"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5622-7402","authenticated-orcid":false,"given":"Lauren J.","family":"Donovan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0089-2411","authenticated-orcid":false,"given":"Chelsie L.","family":"Brewer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5628-145X","authenticated-orcid":false,"given":"Sabrina F.","family":"Bond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleishai Pena","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linus H.","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire E.","family":"Jordan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1302-1911","authenticated-orcid":false,"given":"Oscar C.","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8921-5942","authenticated-orcid":false,"given":"Luis","family":"de Lecea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3362-1642","authenticated-orcid":false,"given":"Julie A.","family":"Kauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4422-2014","authenticated-orcid":false,"given":"Vivianne L.","family":"Tawfik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"issue":"2","key":"2024012412200752000_2024.01.20.576299v1.1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/s41576-019-0183-6","article-title":"The genetics of human ageing","volume":"21","year":"2020","journal-title":"Nat Rev Genet"},{"key":"2024012412200752000_2024.01.20.576299v1.2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.neurobiolaging.2019.12.018","article-title":"The geriatric pain experience in mice: intact cutaneous thresholds but altered responses to tonic and chronic pain","volume":"89","year":"2020","journal-title":"Neurobiol Aging"},{"key":"2024012412200752000_2024.01.20.576299v1.3","doi-asserted-by":"crossref","first-page":"17448069231203090","DOI":"10.1177\/17448069231203090","article-title":"Pain and aging: A unique challenge in neuroinflammation and behavior","volume":"19","year":"2023","journal-title":"Mol Pain"},{"issue":"4","key":"2024012412200752000_2024.01.20.576299v1.4","doi-asserted-by":"crossref","first-page":"e1124","DOI":"10.1002\/jsp2.1124","article-title":"Age-related molecular changes in the lumbar dorsal root ganglia of mice: Signs of sensitization, and inflammatory response","volume":"3","year":"2020","journal-title":"JOR Spine"},{"key":"2024012412200752000_2024.01.20.576299v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2016.05.014"},{"key":"2024012412200752000_2024.01.20.576299v1.6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.04011.x"},{"issue":"7","key":"2024012412200752000_2024.01.20.576299v1.7","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1038\/s43587-022-00246-4","article-title":"Single-cell analysis of the aging female mouse hypothalamus","volume":"2","year":"2022","journal-title":"Nat Aging"},{"key":"2024012412200752000_2024.01.20.576299v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2020.07.002"},{"key":"2024012412200752000_2024.01.20.576299v1.9","doi-asserted-by":"publisher","DOI":"10.1038\/nature13193"},{"key":"2024012412200752000_2024.01.20.576299v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.10.005"},{"key":"2024012412200752000_2024.01.20.576299v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2014.09.018"},{"key":"2024012412200752000_2024.01.20.576299v1.12","doi-asserted-by":"crossref","unstructured":"Muralidharan A , Sotocinal SG , Yousefpour N , Akkurt N , Lima LV , Tansley S , et al. Long-term male-specific chronic pain via telomere- and p53-mediated spinal cord cellular senescence. J Clin Invest. 2022;132(8).","DOI":"10.1172\/JCI151817"},{"key":"2024012412200752000_2024.01.20.576299v1.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.02.031"},{"key":"2024012412200752000_2024.01.20.576299v1.14","doi-asserted-by":"publisher","DOI":"10.1172\/JCI95147"},{"key":"2024012412200752000_2024.01.20.576299v1.15","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00586"},{"issue":"25","key":"2024012412200752000_2024.01.20.576299v1.16","doi-asserted-by":"crossref","first-page":"2235","DOI":"10.1182\/blood.2019003105","article-title":"Altered microRNA expression links IL6 and TNF-induced inflammaging with myeloid malignancy in humans and mice","volume":"135","year":"2020","journal-title":"Blood"},{"key":"2024012412200752000_2024.01.20.576299v1.17","doi-asserted-by":"publisher","DOI":"10.1038\/nature10600"},{"key":"2024012412200752000_2024.01.20.576299v1.18","doi-asserted-by":"publisher","DOI":"10.1038\/nature16932"},{"key":"2024012412200752000_2024.01.20.576299v1.19","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0092-9"},{"key":"2024012412200752000_2024.01.20.576299v1.20","doi-asserted-by":"crossref","unstructured":"Melo Dos Santos LS , Trombetta-Lima M , Bjl E , Demaria M. Cellular senescence in brain aging and neurodegeneration. Ageing Res Rev. 2023:102141.","DOI":"10.1016\/j.arr.2023.102141"},{"issue":"8","key":"2024012412200752000_2024.01.20.576299v1.21","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1038\/s41591-022-01923-y","article-title":"Cellular senescence and senolytics: the path to the clinic","volume":"28","year":"2022","journal-title":"Nat Med"},{"key":"2024012412200752000_2024.01.20.576299v1.22","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.3.2109"},{"key":"2024012412200752000_2024.01.20.576299v1.23","doi-asserted-by":"crossref","unstructured":"Roger L , Tomas F , Gire V . Mechanisms and Regulation of Cellular Senescence. Int J Mol Sci. 2021;22(23).","DOI":"10.3390\/ijms222313173"},{"key":"2024012412200752000_2024.01.20.576299v1.24","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-0801"},{"key":"2024012412200752000_2024.01.20.576299v1.25","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2892"},{"key":"2024012412200752000_2024.01.20.576299v1.26","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2014"},{"key":"2024012412200752000_2024.01.20.576299v1.27","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2018.03.003"},{"key":"2024012412200752000_2024.01.20.576299v1.28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2012.00870.x"},{"key":"2024012412200752000_2024.01.20.576299v1.29","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa5612"},{"key":"2024012412200752000_2024.01.20.576299v1.30","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2022.11.010"},{"key":"2024012412200752000_2024.01.20.576299v1.31","doi-asserted-by":"crossref","first-page":"747067","DOI":"10.3389\/fnins.2021.747067","article-title":"Expression of p16 Within Myenteric Neurons of the Aged Colon: A Potential Marker of Declining Function","volume":"15","year":"2021","journal-title":"Front Neurosci"},{"key":"2024012412200752000_2024.01.20.576299v1.32","doi-asserted-by":"publisher","DOI":"10.1038\/s43587-021-00142-3"},{"issue":"s1","key":"2024012412200752000_2024.01.20.576299v1.33","doi-asserted-by":"crossref","first-page":"S107","DOI":"10.3233\/JAD-161221","article-title":"The DNA Damage Response in Neurons: Die by Apoptosis or Survive in a Senescence-Like State?","volume":"60","year":"2017","journal-title":"J Alzheimers Dis"},{"key":"2024012412200752000_2024.01.20.576299v1.34","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-011-1002-z"},{"key":"2024012412200752000_2024.01.20.576299v1.35","doi-asserted-by":"crossref","unstructured":"Gillispie GJ , Sah E , Krishnamurthy S , Ahmidouch MY , Zhang B , Orr ME . Evidence of the Cellular Senescence Stress Response in Mitotically Active Brain Cells-Implications for Cancer and Neurodegeneration. Life (Basel). 2021;11(2).","DOI":"10.3390\/life11020153"},{"key":"2024012412200752000_2024.01.20.576299v1.36","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0543-y"},{"key":"2024012412200752000_2024.01.20.576299v1.37","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12840"},{"key":"2024012412200752000_2024.01.20.576299v1.38","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2017.11.006"},{"key":"2024012412200752000_2024.01.20.576299v1.39","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pmbts.2015.01.002"},{"key":"2024012412200752000_2024.01.20.576299v1.40","doi-asserted-by":"crossref","unstructured":"Mailhot B , Christin M , Tessandier N , Sotoudeh C , Bretheau F , Turmel R , et al. Neuronal interleukin-1 receptors mediate pain in chronic inflammatory diseases. J Exp Med. 2020;217(9).","DOI":"10.1084\/jem.20191430"},{"key":"2024012412200752000_2024.01.20.576299v1.41","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3858-05.2006"},{"key":"2024012412200752000_2024.01.20.576299v1.42","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1822-09.2009"},{"key":"2024012412200752000_2024.01.20.576299v1.43","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4476-07.2008"},{"key":"2024012412200752000_2024.01.20.576299v1.44","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1186\/1742-2094-8-110","article-title":"Wallerian degeneration: gaining perspective on inflammatory events after peripheral nerve injury","volume":"8","year":"2011","journal-title":"Journal of neuroinflammation"},{"key":"2024012412200752000_2024.01.20.576299v1.45","first-page":"3061","article-title":"Neuraxial Cytokines in Pain States","volume":"10","year":"2019","journal-title":"Frontiers in immunology"},{"key":"2024012412200752000_2024.01.20.576299v1.46","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000158"},{"key":"2024012412200752000_2024.01.20.576299v1.47","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2018.02.001"},{"issue":"1","key":"2024012412200752000_2024.01.20.576299v1.48","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.tcb.2022.04.011","article-title":"The heterogeneity of cellular senescence: insights at the single-cell level","volume":"33","year":"2023","journal-title":"Trends Cell Biol"},{"key":"2024012412200752000_2024.01.20.576299v1.49","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(00)00276-1"},{"key":"2024012412200752000_2024.01.20.576299v1.50","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1999.0814"},{"key":"2024012412200752000_2024.01.20.576299v1.51","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-10-19"},{"key":"2024012412200752000_2024.01.20.576299v1.52","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3881"},{"issue":"10","key":"2024012412200752000_2024.01.20.576299v1.53","doi-asserted-by":"crossref","first-page":"2410","DOI":"10.1097\/j.pain.0000000000001973","article-title":"Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization","volume":"161","year":"2020","journal-title":"Pain"},{"issue":"4","key":"2024012412200752000_2024.01.20.576299v1.54","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.neuron.2019.05.039","article-title":"Deep Sequencing of Somatosensory Neurons Reveals Molecular Determinants of Intrinsic Physiological Properties","volume":"103","year":"2019","journal-title":"Neuron"},{"key":"2024012412200752000_2024.01.20.576299v1.55","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-5836"},{"issue":"5","key":"2024012412200752000_2024.01.20.576299v1.56","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1111\/joim.13141","article-title":"Senolytic drugs: from discovery to translation","volume":"288","year":"2020","journal-title":"J Intern Med"},{"key":"2024012412200752000_2024.01.20.576299v1.57","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12445"},{"key":"2024012412200752000_2024.01.20.576299v1.58","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1404.008"},{"issue":"1","key":"2024012412200752000_2024.01.20.576299v1.59","first-page":"37","article-title":"Specific lipofuscin staining as a novel biomarker to detect replicative and stress-induced senescence","volume":"5","year":"2013","journal-title":"A method applicable in cryo-preserved and archival tissues. Aging (Albany NY"},{"key":"2024012412200752000_2024.01.20.576299v1.60","doi-asserted-by":"crossref","unstructured":"Lind\u00e5 H , Sk\u00f6ld MK , Ochsmann T . Activating transcription factor 3, a useful marker for regenerative response after nerve root injury. Front Neurol. 2011;2:30.","DOI":"10.3389\/fneur.2011.00030"},{"key":"2024012412200752000_2024.01.20.576299v1.61","doi-asserted-by":"crossref","unstructured":"Sah E , Krishnamurthy S , Ahmidouch MY , Gillispie GJ , Milligan C , Orr ME . The Cellular Senescence Stress Response in Post-Mitotic Brain Cells: Cell Survival at the Expense of Tissue Degeneration. Life (Basel). 2021;11(3).","DOI":"10.3390\/life11030229"},{"issue":"4","key":"2024012412200752000_2024.01.20.576299v1.62","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1089\/ars.2020.8048","article-title":"Senescence in Post-Mitotic Cells: A Driver of Aging?","volume":"34","year":"2021","journal-title":"Antioxid Redox Signal"},{"key":"2024012412200752000_2024.01.20.576299v1.63","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2015.1004937"},{"issue":"6","key":"2024012412200752000_2024.01.20.576299v1.64","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1038\/s41419-020-2647-1","article-title":"Aberrant activation of neuronal cell cycle caused by dysregulation of ubiquitin ligase Itch results in neurodegeneration","volume":"11","year":"2020","journal-title":"Cell Death Dis"},{"issue":"6","key":"2024012412200752000_2024.01.20.576299v1.65","first-page":"1933","article-title":"Abnormal expression of the cell cycle regulators P16 and CDK4 in Alzheimer\u2019s disease","volume":"150","year":"1997","journal-title":"Am J Pathol"},{"key":"2024012412200752000_2024.01.20.576299v1.66","doi-asserted-by":"publisher","DOI":"10.1007\/s004010050665"},{"key":"2024012412200752000_2024.01.20.576299v1.67","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-14-05045.2001"},{"key":"2024012412200752000_2024.01.20.576299v1.68","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M311768200"},{"key":"2024012412200752000_2024.01.20.576299v1.69","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.11.012"},{"key":"2024012412200752000_2024.01.20.576299v1.70","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80619-4"},{"key":"2024012412200752000_2024.01.20.576299v1.71","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.2001.0426"},{"key":"2024012412200752000_2024.01.20.576299v1.72","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109334"},{"key":"2024012412200752000_2024.01.20.576299v1.73","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2022.1027116"},{"key":"2024012412200752000_2024.01.20.576299v1.74","doi-asserted-by":"crossref","first-page":"1227705","DOI":"10.3389\/fnins.2023.1227705","article-title":"Senolytic therapy is neuroprotective and improves functional outcome long-term after traumatic brain injury in mice","volume":"17","year":"2023","journal-title":"Front Neurosci"},{"key":"2024012412200752000_2024.01.20.576299v1.75","doi-asserted-by":"crossref","first-page":"101412","DOI":"10.1016\/j.arr.2021.101412","article-title":"Dissecting primary and secondary senescence to enable new senotherapeutic strategies","volume":"70","year":"2021","journal-title":"Ageing Res Rev"},{"issue":"4","key":"2024012412200752000_2024.01.20.576299v1.76","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/j.celrep.2019.03.104","article-title":"Notch Signaling Mediates Secondary Senescence","volume":"27","year":"2019","journal-title":"Cell Rep"},{"issue":"3","key":"2024012412200752000_2024.01.20.576299v1.77","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1007\/s00018-020-03638-0","article-title":"Induction and transmission of oncogene-induced senescence","volume":"78","year":"2021","journal-title":"Cell Mol Life Sci"},{"key":"2024012412200752000_2024.01.20.576299v1.78","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-79090-7_12"},{"key":"2024012412200752000_2024.01.20.576299v1.79","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awh490"},{"key":"2024012412200752000_2024.01.20.576299v1.80","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-16-06289.2000"},{"key":"2024012412200752000_2024.01.20.576299v1.81","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409225102"},{"key":"2024012412200752000_2024.01.20.576299v1.82","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-016-0607-6"},{"issue":"1","key":"2024012412200752000_2024.01.20.576299v1.83","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1152\/jn.00188.2020","article-title":"IL-6 induced upregulation of T-type Ca(2+) currents and sensitization of DRG nociceptors is attenuated by MNK inhibition","volume":"124","year":"2020","journal-title":"J Neurophysiol"},{"key":"2024012412200752000_2024.01.20.576299v1.84","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03050.x"},{"key":"2024012412200752000_2024.01.20.576299v1.85","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(00)00331-6"},{"key":"2024012412200752000_2024.01.20.576299v1.86","doi-asserted-by":"crossref","unstructured":"Liu JA , Yu J , Cheung CW . Immune Actions on the Peripheral Nervous System in Pain. Int J Mol Sci. 2021;22(3).","DOI":"10.3390\/ijms22031448"},{"key":"2024012412200752000_2024.01.20.576299v1.87","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1995.64020850.x"},{"key":"2024012412200752000_2024.01.20.576299v1.88","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2011.07230.x"},{"key":"2024012412200752000_2024.01.20.576299v1.89","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aet216"},{"issue":"4","key":"2024012412200752000_2024.01.20.576299v1.90","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.nec.2014.07.006","article-title":"Nerve blocks for chronic pain","volume":"25","year":"2014","journal-title":"Neurosurg Clin N Am"},{"issue":"1","key":"2024012412200752000_2024.01.20.576299v1.91","doi-asserted-by":"crossref","first-page":"14170","DOI":"10.1038\/s41598-020-71042-6","article-title":"Depletion of senescent-like neuronal cells alleviates cisplatin-induced peripheral neuropathy in mice","volume":"10","year":"2020","journal-title":"Sci Rep"},{"issue":"1","key":"2024012412200752000_2024.01.20.576299v1.92","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1186\/s11658-023-00474-5","article-title":"Astrocyte senescence-like response related to peripheral nerve injury-induced neuropathic pain","volume":"28","year":"2023","journal-title":"Cell Mol Biol Lett"},{"key":"2024012412200752000_2024.01.20.576299v1.93","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13089"},{"key":"2024012412200752000_2024.01.20.576299v1.94","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"2024012412200752000_2024.01.20.576299v1.95","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(94)90144-9"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.01.20.576299","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T05:49:21Z","timestamp":1768456161000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2024.01.20.576299"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,22]]},"references-count":95,"URL":"https:\/\/doi.org\/10.1101\/2024.01.20.576299","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1038\/s41593-025-01954-x","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2024,1,22]]},"subtype":"preprint"}}