{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T16:48:39Z","timestamp":1775926119411,"version":"3.50.1"},"posted":{"date-parts":[[2024,1,25]]},"group-title":"Genetics","reference-count":33,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,1,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Intramuscular injection of Wnt7a has been shown to accelerate and augment skeletal muscle regeneration and to ameliorate dystrophic progression in\n                  <jats:italic>mdx<\/jats:italic>\n                  muscle, a model for Duchenne muscular dystrophy (DMD). However, loss-of-function studies to investigate the requirement for Wnt7a in muscle regeneration has not been evaluated. Here, we assessed muscle regeneration and function in wild type (WT) and\n                  <jats:italic>mdx<\/jats:italic>\n                  mice where Wnt7a was specifically deleted in muscle using a conditional\n                  <jats:italic>Wnt7a<\/jats:italic>\n                  floxed allele and a\n                  <jats:italic>Myf5-Cre<\/jats:italic>\n                  driver. We found that both WT and\n                  <jats:italic>mdx<\/jats:italic>\n                  mice with deletion of Wnt7a in muscle, exhibited marked deficiencies in muscle regeneration at 21 d following cardiotoxin (CTX) induced injury. Unlike WT, deletion of Wnt7a in\n                  <jats:italic>mdx<\/jats:italic>\n                  resulted in a marked decrease in specific force generation prior to CTX injury. However, both WT and\n                  <jats:italic>mdx<\/jats:italic>\n                  muscle lacking Wnt7a displayed decreased specific force generation following CTX injection. Notably the regeneration deficit observed in\n                  <jats:italic>mdx<\/jats:italic>\n                  mice lacking Wnt7a in muscle was rescued by a single tail vein injection of an extracellular vesicle preparation containing Wnt7a (Wnt7a-EVs). Therefore, we conclude that the regenerative capacity of muscle in\n                  <jats:italic>mdx<\/jats:italic>\n                  mice is due to the upregulation of endogenous Wnt7a following injury, and that systemic delivery of Wnt7a-EVs represents a therapeutic strategy for treating DMD.\n                <\/jats:p>","DOI":"10.1101\/2024.01.24.577041","type":"posted-content","created":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T23:15:11Z","timestamp":1706224511000},"source":"Crossref","is-referenced-by-count":4,"title":["Wnt7a is Required for Regeneration of Dystrophic Skeletal Muscle"],"prefix":"10.64898","author":[{"given":"Uxia","family":"Gurriaran-Rodriguez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kasun","family":"Kodippili","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Datzkiw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ehsan","family":"Javandoost","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Teresa","family":"Rejas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3866-5249","authenticated-orcid":false,"given":"Michael A.","family":"Rudnicki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024013002300553000_2024.01.24.577041v1.1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201310035"},{"key":"2024013002300553000_2024.01.24.577041v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.03.013"},{"key":"2024013002300553000_2024.01.24.577041v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2404"},{"key":"2024013002300553000_2024.01.24.577041v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3990"},{"key":"2024013002300553000_2024.01.24.577041v1.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1215765109"},{"key":"2024013002300553000_2024.01.24.577041v1.6","doi-asserted-by":"publisher","DOI":"10.1126\/science.1218693"},{"key":"2024013002300553000_2024.01.24.577041v1.7","doi-asserted-by":"publisher","DOI":"10.1101\/2023.05.30.542914"},{"key":"2024013002300553000_2024.01.24.577041v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.125"},{"key":"2024013002300553000_2024.01.24.577041v1.9","doi-asserted-by":"crossref","unstructured":"R. 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