{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:50:26Z","timestamp":1768510226444,"version":"3.49.0"},"posted":{"date-parts":[[2024,1,30]]},"group-title":"Developmental Biology","reference-count":72,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,1,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Purpose<\/jats:title>\n                  <jats:p>Despite strong evidence demonstrating that normal lens development requires regulation governed by miRNAs, the functional role of specific miRNAs in mammalian lens development remains largely unexplored.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A comprehensive analysis of miRNA transcripts in the newborn mouse lens, exploring both differential expression between lens epithelial cells and lens fiber cells and overall miRNA abundance was conducted by miRNA-seq. Mouse lenses lacking each of three abundantly expressed lens miRNAs: miR-184, miR-26 and miR-1 were analyzed to explore the role of these miRNAs in lens development.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    Mice lacking all three copies of\n                    <jats:italic>miR-26<\/jats:italic>\n                    (\n                    <jats:italic>\n                      miR-26\n                      <jats:sup>TKO<\/jats:sup>\n                    <\/jats:italic>\n                    ) developed postnatal cataracts as early as 4-6 weeks of age. RNA-seq analysis of neonatal lenses from\n                    <jats:italic>\n                      miR-26\n                      <jats:sup>TKO<\/jats:sup>\n                    <\/jats:italic>\n                    mice exhibited abnormal reduced expression of a cohort of genes found to be lens-enriched and linked to cataract (\n                    <jats:italic>e.g. Foxe3<\/jats:italic>\n                    ,\n                    <jats:italic>Hsf4<\/jats:italic>\n                    ,\n                    <jats:italic>Mip<\/jats:italic>\n                    ,\n                    <jats:italic>Tdrd7,<\/jats:italic>\n                    and numerous crystallin genes), and abnormal elevated expression of genes related to neural development (\n                    <jats:italic>Lhx3, Neurod4, Shisa7, Elavl3<\/jats:italic>\n                    ), inflammation (\n                    <jats:italic>Ccr1, Tnfrsf12a, Csf2ra)<\/jats:italic>\n                    , the complement pathway, and epithelial to mesenchymal transition (\n                    <jats:italic>Tnfrsf1a, Ccl7, Stat3, Cntfr<\/jats:italic>\n                    ).\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>miR-1, miR-184 and miR-26 are each dispensable for normal embryonic lens development. However, loss of miR-26 causes lens transcriptome changes and drives cataract formation.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2024.01.29.577818","type":"posted-content","created":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T22:25:19Z","timestamp":1706653519000},"source":"Crossref","is-referenced-by-count":0,"title":["miR-26 deficiency causes alterations in lens transcriptome and results in adult-onset cataract"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8502-6506","authenticated-orcid":false,"given":"Anil","family":"Upreti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7666-3842","authenticated-orcid":false,"given":"Thanh 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