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Here, we show that a\n                  <jats:italic>\n                    Tbx5\n                    <jats:sup>+<\/jats:sup>\n                    \/Mef2cAHF\n                  <\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  progenitor lineage forms a compartment boundary bisecting the IVS. This coordinated population originates at a first- and second heart field interface. Ablation of\n                  <jats:italic>\n                    Tbx5\n                    <jats:sup>+<\/jats:sup>\n                    \/Mef2cAHF\n                  <\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  progenitors cause IVS disorganization, right ventricular hypoplasia and mixing of IVS lineages. Reduced dosage of the CHD transcription factor TBX5 disrupts boundary position and integrity, resulting in ventricular septation defects (VSDs) and patterning defects, including misexpression of\n                  <jats:italic>Slit2<\/jats:italic>\n                  and\n                  <jats:italic>Ntn1<\/jats:italic>\n                  , which encode guidance cues. Reducing NTN1 dosage partly rescues cardiac defects in\n                  <jats:italic>Tbx5<\/jats:italic>\n                  mutant embryos. Loss of\n                  <jats:italic>Slit2<\/jats:italic>\n                  or\n                  <jats:italic>Ntn1<\/jats:italic>\n                  causes VSDs and perturbed septal lineage distributions. Thus, we identify\n                  <jats:italic>Tbx5<\/jats:italic>\n                  as a candidate selector gene, directing progenitors and regulating essential cues, to pattern a compartment boundary for proper cardiac septation, revealing mechanisms for cardiac birth defects.\n                <\/jats:p>","DOI":"10.1101\/2024.02.05.578995","type":"posted-content","created":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T02:40:10Z","timestamp":1707187210000},"source":"Crossref","is-referenced-by-count":5,"title":["A disrupted compartment boundary underlies abnormal cardiac patterning and congenital heart defects"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1207-1490","authenticated-orcid":false,"given":"Irfan S.","family":"Kathiriya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin H.","family":"Dominguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5401-6989","authenticated-orcid":false,"given":"Kavitha S.","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7405-6213","authenticated-orcid":false,"given":"Jonathon M.","family":"Muncie-Vasic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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