{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T21:39:28Z","timestamp":1783633168308,"version":"3.55.0"},"posted":{"date-parts":[[2024,2,8]]},"group-title":"Physiology","reference-count":55,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,4,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>The crypt-villus structure of the small intestine serves as an essential protective barrier, with its integrity monitored by the gut\u2019s sensory system. Enterochromaffin (EC) cells, which are rare sensory epithelial cells that release serotonin (5-HT), surveil the mucosal environment and signal both within and outside the gut. However, it remains unclear whether EC cells in intestinal crypts and villi respond to different stimuli and elicit distinct responses. In this study, we introduce a new reporter mouse model to observe the release and propagation of serotonin in live intestines. Using this system, we show that crypt EC cells exhibit two modes of serotonin release: transient receptor potential A1 (TRPA1)-dependent tonic serotonin release that controls basal ionic secretion, and irritant-evoked serotonin release that activates gut sensory neurons. Furthermore, we find that a thick protective mucus layer prevents TRPA1 receptors on crypt EC cells from responding to luminal irritants such as reactive electrophiles; if this mucus layer is compromised, then crypt EC cells become susceptible to activation by luminal irritants. On the other hand, villus EC cells detect oxidative stress through TRPM2 channels and co-release serotonin and ATP to activate nearby gut sensory fibers. Our work highlights the physiological importance of intestinal architecture and differential TRP channel expression in sensing noxious stimuli that elicit nausea and\/or pain sensations in the gut.<\/jats:p>","DOI":"10.1101\/2024.02.06.579180","type":"posted-content","created":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T20:45:41Z","timestamp":1707425141000},"source":"Crossref","is-referenced-by-count":3,"title":["Crypt and Villus Enterochromaffin Cells are Distinct Stress Sensors in the Gut"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3167-9784","authenticated-orcid":false,"given":"Kouki K.","family":"Touhara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1103-0833","authenticated-orcid":false,"given":"Nathan D.","family":"Rossen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1997-4572","authenticated-orcid":false,"given":"Fei","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tifany","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1562-4137","authenticated-orcid":false,"given":"Andrea M.","family":"Harrington","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5038-0820","authenticated-orcid":false,"given":"Sonia","family":"Garcia Caraballo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8270-7215","authenticated-orcid":false,"given":"Mariana","family":"Brizuela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracey","family":"O\u2019Donnell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5178-0199","authenticated-orcid":false,"given":"Onur","family":"Cil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2527-2905","authenticated-orcid":false,"given":"Stuart M.","family":"Brierley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9166-9919","authenticated-orcid":false,"given":"Yulong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Julius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024042702200544000_2024.02.06.579180v2.1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-021115-105439"},{"key":"2024042702200544000_2024.02.06.579180v2.2","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1038\/169800b0","article-title":"Identification of enteramine, the specific hormone of the enterochromaffin cell system, as 5-hydroxytryptamine","volume":"169","year":"1952","journal-title":"Nature"},{"key":"2024042702200544000_2024.02.06.579180v2.3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805323106"},{"key":"2024042702200544000_2024.02.06.579180v2.4","doi-asserted-by":"publisher","DOI":"10.1097\/MED.0b013e32835bc703"},{"key":"2024042702200544000_2024.02.06.579180v2.5","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.105"},{"key":"2024042702200544000_2024.02.06.579180v2.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.05.034"},{"key":"2024042702200544000_2024.02.06.579180v2.7","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.immuni.2020.10.014","article-title":"Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis","volume":"54","year":"2021","journal-title":"Immunity"},{"key":"2024042702200544000_2024.02.06.579180v2.8","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-15834-3"},{"key":"2024042702200544000_2024.02.06.579180v2.9","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/978-3-031-05843-1_30","article-title":"Epithelial 5-HT4 receptors as a target for treating constipation and intestinal inflammation","volume":"1383","year":"2022","journal-title":"Adv. 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