{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T11:18:10Z","timestamp":1774610290688,"version":"3.50.1"},"posted":{"date-parts":[[2024,2,19]]},"group-title":"Biochemistry","reference-count":50,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,3,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Rhodanese-like domains (RLDs) represent a widespread protein family canonically involved in sulfur transfer reactions between diverse donor and acceptor molecules. RLDs mediate these transsulfuration reactions via a transient persulfide intermediate, created by modifying a conserved cysteine residue in their active sites. RLDs are involved in various aspects of sulfur metabolism, including sulfide oxidation in mitochondria, iron-sulfur cluster biogenesis, and thio-cofactor biosynthesis. However, due to the inherent complexity of sulfur metabolism caused by the intrinsically high nucleophilicity and redox sensitivity of thiol-containing compounds, the physiological functions of many RLDs remain to be explored. Here, we focus on a single domain\n                  <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                  RLD (Ab-RLD) associated with a desulfurase encapsulin which is able to store substantial amounts of sulfur inside its protein shell. We determine the 1.6 \u00c5 x-ray crystal structure of Ab-RLD, highlighting a homodimeric structure with a number of unusual features. We show through kinetic analysis that Ab-RLD exhibits thiosulfate sulfurtransferase activity with both cyanide and glutathione acceptors. Using native mass spectrometry and\n                  <jats:italic>in vitro<\/jats:italic>\n                  assays, we provide evidence that Ab-RLD can stably carry a persulfide and thiosulfate modification and may employ a ternary catalytic mechanism. Our results will inform future studies aimed at investigating the functional link between Ab-RLD and the desulfurase encapsulin.\n                <\/jats:p>","DOI":"10.1101\/2024.02.19.581022","type":"posted-content","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T23:00:12Z","timestamp":1708383612000},"source":"Crossref","is-referenced-by-count":1,"title":["Structural and biochemical characterization of an encapsulin-associated rhodanese from\n                  <i>Acinetobacter baumannii<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0612-7606","authenticated-orcid":false,"given":"Robert","family":"Benisch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6328-2031","authenticated-orcid":false,"given":"Tobias W.","family":"Giessen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024030800250628000_2024.02.19.581022v2.1","doi-asserted-by":"publisher","DOI":"10.1080\/15216540701206859"},{"key":"2024030800250628000_2024.02.19.581022v2.2","doi-asserted-by":"publisher","DOI":"10.1038\/273124a0"},{"key":"2024030800250628000_2024.02.19.581022v2.3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV120.011304"},{"key":"2024030800250628000_2024.02.19.581022v2.4","doi-asserted-by":"publisher","DOI":"10.3891\/acta.chem.scand.07-1137"},{"key":"2024030800250628000_2024.02.19.581022v2.5","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.675694"},{"key":"2024030800250628000_2024.02.19.581022v2.6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.282368"},{"key":"2024030800250628000_2024.02.19.581022v2.7","doi-asserted-by":"publisher","DOI":"10.1021\/bi0503448"},{"key":"2024030800250628000_2024.02.19.581022v2.8","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106907200"},{"key":"2024030800250628000_2024.02.19.581022v2.9","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12499"},{"key":"2024030800250628000_2024.02.19.581022v2.10","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02159-14"},{"key":"2024030800250628000_2024.02.19.581022v2.11","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.8.2277-2284.2000"},{"key":"2024030800250628000_2024.02.19.581022v2.12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA117.000826"},{"key":"2024030800250628000_2024.02.19.581022v2.13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2008.06535.x"},{"key":"2024030800250628000_2024.02.19.581022v2.14","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adk9345"},{"key":"2024030800250628000_2024.02.19.581022v2.15","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-040320-102858"},{"key":"2024030800250628000_2024.02.19.581022v2.16","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2017.29"},{"key":"2024030800250628000_2024.02.19.581022v2.17","doi-asserted-by":"publisher","DOI":"10.1080\/10409238.2017.1337709"},{"key":"2024030800250628000_2024.02.19.581022v2.18","doi-asserted-by":"publisher","DOI":"10.1039\/d3tb00288h"},{"key":"2024030800250628000_2024.02.19.581022v2.19","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1473"},{"key":"2024030800250628000_2024.02.19.581022v2.20","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.46070"},{"key":"2024030800250628000_2024.02.19.581022v2.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2022.01.008"},{"key":"2024030800250628000_2024.02.19.581022v2.22","doi-asserted-by":"publisher","DOI":"10.1101\/2023.09.18.558302"},{"key":"2024030800250628000_2024.02.19.581022v2.23","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.74358"},{"key":"2024030800250628000_2024.02.19.581022v2.24","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.59288"},{"key":"2024030800250628000_2024.02.19.581022v2.25","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.100950"},{"key":"2024030800250628000_2024.02.19.581022v2.26","doi-asserted-by":"crossref","first-page":"4016","DOI":"10.1016\/S0021-9258(18)51822-6","article-title":"Mechanism of Rhodanese Catalysis of Thiosulfate-Lipoate Oxidation-Reduction","volume":"238","year":"1963","journal-title":"J Biol Chem"},{"key":"2024030800250628000_2024.02.19.581022v2.27","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.790170"},{"key":"2024030800250628000_2024.02.19.581022v2.28","doi-asserted-by":"crossref","first-page":"2418","DOI":"10.1016\/S0021-9258(19)78240-4","article-title":"The purification and characterization of O-acetylserine sulfhydrylase-A from Salmonella typhimurium","volume":"244","year":"1969","journal-title":"J Biol Chem"},{"key":"2024030800250628000_2024.02.19.581022v2.29","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-0590(83)80008-6"},{"key":"2024030800250628000_2024.02.19.581022v2.30","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(01)00666-9"},{"key":"2024030800250628000_2024.02.19.581022v2.31","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2014.11.016"},{"key":"2024030800250628000_2024.02.19.581022v2.32","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2020.165716"},{"key":"2024030800250628000_2024.02.19.581022v2.33","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2008.08.006"},{"key":"2024030800250628000_2024.02.19.581022v2.34","doi-asserted-by":"publisher","DOI":"10.3390\/biom10091245"},{"key":"2024030800250628000_2024.02.19.581022v2.35","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21121721"},{"key":"2024030800250628000_2024.02.19.581022v2.36","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00205"},{"key":"2024030800250628000_2024.02.19.581022v2.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.11.013"},{"key":"2024030800250628000_2024.02.19.581022v2.38","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2530263.x"},{"key":"2024030800250628000_2024.02.19.581022v2.39","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2009.12.013"},{"key":"2024030800250628000_2024.02.19.581022v2.40","doi-asserted-by":"publisher","DOI":"10.2174\/1874285800802010018"},{"key":"2024030800250628000_2024.02.19.581022v2.41","doi-asserted-by":"publisher","DOI":"10.1021\/bi500650h"},{"key":"2024030800250628000_2024.02.19.581022v2.42","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2023.168018"},{"key":"2024030800250628000_2024.02.19.581022v2.43","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.9b00735"},{"key":"2024030800250628000_2024.02.19.581022v2.44","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"2024030800250628000_2024.02.19.581022v2.45","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3290"},{"key":"2024030800250628000_2024.02.19.581022v2.46","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909047337"},{"key":"2024030800250628000_2024.02.19.581022v2.47","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798319011471"},{"key":"2024030800250628000_2024.02.19.581022v2.48","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01488-1"},{"key":"2024030800250628000_2024.02.19.581022v2.49","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3235"},{"key":"2024030800250628000_2024.02.19.581022v2.50","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)43009-7"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.02.19.581022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T05:58:27Z","timestamp":1768456707000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2024.02.19.581022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,19]]},"references-count":50,"URL":"https:\/\/doi.org\/10.1101\/2024.02.19.581022","relation":{},"subject":[],"published":{"date-parts":[[2024,2,19]]},"subtype":"preprint"}}