{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:56:51Z","timestamp":1768510611469,"version":"3.49.0"},"posted":{"date-parts":[[2024,2,29]]},"group-title":"Cell Biology","reference-count":70,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,2,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Doublecortin (DCX) is a microtubule-associated protein critical for brain development. Although most highly expressed in the developing central nervous system, the molecular function of DCX in neuron morphogenesis remains unknown and controversial. We demonstrate that DCX function is intimately linked to its microtubule-binding activity. By using human induced pluripotent stem cell (hiPSC)- derived cortical i\n                  <jats:sup>3<\/jats:sup>\n                  Neurons genome engineered to express mEmerald-tagged DCX from the endogenous locus, we find that DCX-MT interactions become highly polarized very early during neuron morphogenesis. DCX becomes enriched only on straight microtubules in advancing growth cones with approximately 120 DCX molecules bound per micrometer of growth cone microtubule. At a similar saturation, microtubule-bound DCX molecules begin to impede lysosome transport, and thus can potentially control growth cone organelle entry. In addition, by comparing control, DCX-mEmerald and knockout DCX -\/Y i\n                  <jats:sup>3<\/jats:sup>\n                  Neurons, we find that DCX stabilizes microtubules in the growth cone peripheral domain by reducing the microtubule catastrophe frequency and the depolymerization rate. DCX -\/Y i\n                  <jats:sup>3<\/jats:sup>\n                  Neuron morphogenesis was inhibited in soft microenvironments that mimic the viscoelasticity of brain tissue and DCX -\/Y neurites failed to grow toward brain-derived neurotrophic factor (BDNF) gradients. Together with high resolution traction force microscopy data, we propose a model in which DCX-decorated, rigid growth cone microtubules provide intracellular mechanical resistance to actomyosin generated contractile forces in soft physiological environments in which weak and transient adhesion-mediated forces in the growth cone periphery may be insufficient for productive growth cone advance. These data provide a new mechanistic understanding of how DCX mutations cause lissencephaly-spectrum brain malformations by impacting growth cone dynamics during neuron morphogenesis in physiological environments.\n                <\/jats:p>","DOI":"10.1101\/2024.02.28.582626","type":"posted-content","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T03:35:12Z","timestamp":1709264112000},"source":"Crossref","is-referenced-by-count":5,"title":["Doublecortin reinforces microtubules to promote growth cone advance in soft environments"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0976-9396","authenticated-orcid":false,"given":"Alessandro","family":"Dema","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0056-0449","authenticated-orcid":false,"given":"Rabab 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