{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T21:02:58Z","timestamp":1768510978600,"version":"3.49.0"},"posted":{"date-parts":[[2024,3,27]]},"group-title":"Cell Biology","reference-count":58,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,3,27]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                <jats:p>\n                  Regeneration of dentin and odontoblasts from dental pulp stem cells (DPSCs) is essential for permanent tooth maintenance. However, the\n                  <jats:italic>in vivo<\/jats:italic>\n                  identity and role of endogenous DPSCs in reparative dentinogenesis are elusive. Here, using pulp single-cell analysis before and after molar eruption, we revealed that endogenous DPSCs are enriched in\n                  <jats:italic>Cxcl12-<\/jats:italic>\n                  GFP\n                  <jats:sup>+<\/jats:sup>\n                  coronal papilla-like cells with\n                  <jats:italic>Mx1-<\/jats:italic>\n                  Cre labeling. These\n                  <jats:italic>Mx1<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  <jats:italic>Cxcl12-<\/jats:italic>\n                  GFP\n                  <jats:sup>+<\/jats:sup>\n                  cells are long-term repopulating cells that contribute to the majority of pulp cells and new odontoblasts after eruption. Upon molar injury,\n                  <jats:italic>Mx1<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  DPSCs localize into the injury site and differentiate into new odontoblasts, forming\n                  <jats:italic>scleraxis<\/jats:italic>\n                  -GFP\n                  <jats:sup>+<\/jats:sup>\n                  and\n                  <jats:italic>osteocalcin<\/jats:italic>\n                  -GFP\n                  <jats:sup>+<\/jats:sup>\n                  dentinal tubules and reparative dentin. Single-cell and FACS analysis showed that\n                  <jats:italic>Mx1<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  <jats:italic>Cxcl12-<\/jats:italic>\n                  GFP\n                  <jats:sup>+<\/jats:sup>\n                  DPSCs are the most primitive cells with stem cell marker expression and odontoblast differentiation. Taken together, our findings demonstrate that\n                  <jats:italic>Mx1<\/jats:italic>\n                  labels postnatal DSPCs, which are the main source of pulp cells and new odontoblasts with reparative dentinogenesis\n                  <jats:italic>in vivo<\/jats:italic>\n                  .\n                <\/jats:p>","DOI":"10.1101\/2024.03.21.586156","type":"posted-content","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T04:55:26Z","timestamp":1711515326000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Mx1<\/i>\n                  -labeled pulp progenitor cells are main contributors to postnatal odontoblasts and pulp cells in murine molars"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9030-597X","authenticated-orcid":false,"given":"Dongwook","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2884-5702","authenticated-orcid":false,"given":"YoungJae","family":"Jeong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8036-8459","authenticated-orcid":false,"given":"Laura","family":"Ortinau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7161-2106","authenticated-orcid":false,"given":"Jea","family":"Solidum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2570-3250","authenticated-orcid":false,"given":"Dongsu","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024032811001756000_2024.03.21.586156v1.1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411960070020101"},{"key":"2024032811001756000_2024.03.21.586156v1.2","doi-asserted-by":"publisher","DOI":"10.1002\/term.319"},{"key":"2024032811001756000_2024.03.21.586156v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2017.02.022"},{"key":"2024032811001756000_2024.03.21.586156v1.4","doi-asserted-by":"publisher","DOI":"10.1007\/s12257-008-0146-9"},{"key":"2024032811001756000_2024.03.21.586156v1.5","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01685"},{"key":"2024032811001756000_2024.03.21.586156v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2021.116010"},{"key":"2024032811001756000_2024.03.21.586156v1.7","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.8.1671"},{"key":"2024032811001756000_2024.03.21.586156v1.8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2006.01346.x"},{"key":"2024032811001756000_2024.03.21.586156v1.9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-263X.2008.00964.x"},{"key":"2024032811001756000_2024.03.21.586156v1.10","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-03145-6"},{"key":"2024032811001756000_2024.03.21.586156v1.11","doi-asserted-by":"publisher","DOI":"10.1177\/0022034516678208"},{"key":"2024032811001756000_2024.03.21.586156v1.12","doi-asserted-by":"publisher","DOI":"10.1038\/nature13536"},{"key":"2024032811001756000_2024.03.21.586156v1.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.06.006"},{"key":"2024032811001756000_2024.03.21.586156v1.14","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11277"},{"key":"2024032811001756000_2024.03.21.586156v1.15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1810200115"},{"key":"2024032811001756000_2024.03.21.586156v1.16","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32490-y"},{"key":"2024032811001756000_2024.03.21.586156v1.17","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-019-0060-3"},{"key":"2024032811001756000_2024.03.21.586156v1.18","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.082867"},{"key":"2024032811001756000_2024.03.21.586156v1.19","doi-asserted-by":"publisher","DOI":"10.1089\/biores.2019.0046"},{"key":"2024032811001756000_2024.03.21.586156v1.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2009.01.005"},{"key":"2024032811001756000_2024.03.21.586156v1.21","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510363373"},{"key":"2024032811001756000_2024.03.21.586156v1.22","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517729811"},{"key":"2024032811001756000_2024.03.21.586156v1.23","doi-asserted-by":"publisher","DOI":"10.21203\/rs.3.rs-1566145\/v1"},{"key":"2024032811001756000_2024.03.21.586156v1.24","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.595593"},{"key":"2024032811001756000_2024.03.21.586156v1.25","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2022.03.012"},{"key":"2024032811001756000_2024.03.21.586156v1.26","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511424409"},{"key":"2024032811001756000_2024.03.21.586156v1.27","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2017.3254"},{"key":"2024032811001756000_2024.03.21.586156v1.28","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10352"},{"key":"2024032811001756000_2024.03.21.586156v1.29","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.06.008"},{"key":"2024032811001756000_2024.03.21.586156v1.30","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.02.003"},{"key":"2024032811001756000_2024.03.21.586156v1.31","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2019.11.003"},{"key":"2024032811001756000_2024.03.21.586156v1.32","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(01)00683-4"},{"key":"2024032811001756000_2024.03.21.586156v1.33","doi-asserted-by":"publisher","DOI":"10.3791\/51289"},{"key":"20240328110017560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