{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T21:07:34Z","timestamp":1768511254378,"version":"3.49.0"},"posted":{"date-parts":[[2024,3,23]]},"group-title":"Microbiology","reference-count":45,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,3,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The obligate intracellular parasite\n                  <jats:italic>Toxoplasma gondii<\/jats:italic>\n                  can infect and replicate in any warm-blooded cell tested to date, but much of our knowledge about\n                  <jats:italic>T. gondii<\/jats:italic>\n                  cell biology comes from just one host cell type: human foreskin fibroblasts (HFFs). To expand our knowledge of host-parasite lipid interactions, we studied\n                  <jats:italic>T. gondii<\/jats:italic>\n                  in intestinal epithelial cells, the first site of host-parasite contact following oral infection and the exclusive site of parasite sexual development in feline hosts. We found that highly metabolic Caco-2 cells are permissive to\n                  <jats:italic>T. gondii<\/jats:italic>\n                  growth even when treated with high levels of linoleic acid (LA), a polyunsaturated fatty acid (PUFA) that kills parasites in HFFs. Caco-2 cells appear to sequester LA away from the parasite, preventing membrane disruptions and lipotoxicity that characterize LA-induced parasite death in HFFs. Our work is an important step toward understanding host-parasite interactions in feline intestinal epithelial cells, an understudied but important cell type in the\n                  <jats:italic>T. gondii<\/jats:italic>\n                  life cycle.\n                <\/jats:p>","DOI":"10.1101\/2024.03.22.586332","type":"posted-content","created":{"date-parts":[[2024,3,23]],"date-time":"2024-03-23T23:55:19Z","timestamp":1711238119000},"source":"Crossref","is-referenced-by-count":0,"title":["Host cell-specific metabolism of linoleic acid controls\n                  <i>Toxoplasma gondii<\/i>\n                  growth in cell culture"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6602-6399","authenticated-orcid":false,"given":"Nicole 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